Triple gene block: modular design of a multifunctional machine for plant virus movement

Morozov, Sergey Y.; Solovyev, Andrey G.

doi:10.1099/vir.0.18922-0

Cited by 332 publications

(375 citation statements)

References 181 publications

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“…2). The TGB3 'signature motif', C(X 5 )G(X 6-9 )C (Morozov et al, 1991;Morozov & Solovyev, 2003), was present in all sequences. PVX TGB3 has predicted 23 aa N-terminal transmembrane and C-terminal 45 aa cytoplasmic segments (Krishnamurthy et al, 2003).…”

Section: Subcellular Localization Of Pvx and Altmv Tgb3mentioning

confidence: 94%

“…Despite this body of work, 'a full understanding of the specific requirement of TGB3 and the functional interchangeability of TGB3 among different potexviruses for intercellular movement remains elusive' (Lin et al, 2006). The 8 kDa PVX TGB3 is an ER-binding protein with a single, N-terminal transmembrane domain (Morozov & Solovyev, 2003;Verchot-Lubicz et al, 2007), and TGB2 and TGB3 are reported to co-localize (Ju et al, 2008;Solovyev et al, 2000). PVX TGB3 interacts with the ER network and is associated with granular vesicles induced by TGB2 (Samuels et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…These have been differentiated into 'class I' hordei-like viruses, which have rigid, rod-shaped particles, and 'class II' potex-like viruses, having flexuous particles [exemplified by Potato virus X (PVX), type member of the genus Potexvirus] (reviewed by Morozov & Solovyev, 2003). Potexviruses have positivesense, single-stranded, monopartite RNA genomes of approximately 5.9-7.0 kb with five gene products (Adams et al, 2004;Fig.…”

Section: Introductionmentioning

confidence: 99%

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Mutation of a chloroplast-targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long-distance virus movement

Lim¹,

Vaira²,

Bae

et al. 2010

Journal of General Virology

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Cell-to-cell movement of potexviruses requires coordinated action of the coat protein and triple gene block (TGB) proteins. The structural properties of Alternanthera mosaic virus (AltMV) TGB3 were examined by methods differentiating between signal peptides and transmembrane domains, and its subcellular localization was studied by Agrobacterium-mediated transient expression and confocal microscopy. Unlike potato virus X (PVX) TGB3, AltMV TGB3 was not associated with the endoplasmic reticulum, and accumulated preferentially in mesophyll cells. Deletion and sitespecific mutagenesis revealed an internal signal VL(17,18) of TGB3 essential for chloroplast localization, and either deletion of the TGB3 start codon or alteration of the chloroplastlocalization signal limited cell-to-cell movement to the epidermis, yielding a virus that was unable to move into the mesophyll layer. Overexpression of AltMV TGB3 from either AltMV or PVX infectious clones resulted in veinal necrosis and vesiculation at the chloroplast membrane, a cytopathology not observed in wild-type infections. The distinctive mesophyll and chloroplast localization of AltMV TGB3 highlights the critical role played by mesophyll targeting in virus longdistance movement within plants.

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Section: Subcellular Localization Of Pvx and Altmv Tgb3mentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mutation of a chloroplast-targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long-distance virus movement

Lim¹,

Vaira²,

Bae

et al. 2010

Journal of General Virology

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“…ORF 1 encodes the RNA-dependent RNA polymerase (RdRp; 166 kDa), which is required for replication and the production of three subgenomic RNAs. ORFs 2-4 are known as the triple gene block (TGB), encoding three polypeptides of 25, 12, and 8 kDa, respectively and are involved in viral movement [16,24]. The TGB proteins are highly conserved in members of the genera Potexvirus, Hordeivirus, Benyvirus and Carlavirus [6,15,23].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and field survey of Iranian potato virus X isolates

et al. 2014

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Six hundred and one symptomatic potato samples were collected from nine provinces in Iran. Screening by double-antibody sandwich enzyme linked immunosorbent assay using a potato virus X (PVX) together with a few potyviruses polyclonal antibodies, produced positive reactions in 4.3 % of samples against PVX. Based on symptoms on different test plant, the isolates were divided into two groups: the first groups causing blistering and malformation of leaves and the second showed mild mosaic and vein clearing in Nicotiana glutinosa. The almost complete nucleotide sequence of two isolates as a representative of severe and a mild isolates were determined. Genomes of two PVX Iranian isolates are identical to that of the most PVX isolates comprise 6435 nucleotides in length excluding 101 nucleotide in the 5 0 end of the genome and shares 94.8-96.7 % identities with European and Asian, and 77-96.1 % with American isolates. Furthermore, the 3 0 -terminal sequences, including the coat protein coding region of other 13 Iranian isolates were determined and compared with the GenBank sequences. Phylogenetic analysis of the cp gene of 13 Iranian isolates together all those available in public databases indicated that the 13 Iranian isolates all belong to low diversity clade I.

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“…Virions or viral RNA can be transported to PD during later stages of infection inside the cell. If TGBp2 and TGBp3 coordinate the transport of TGBp1-CP-vRNA complex along ER into PD (Morozov and Solovyev 2003;Lucas 2006), available data illustrating that vesicles inducing TGBp2 PVX are vital for cell-to-cell transfer (Ju et al 2007) bring to mind a question about the contents of these vesicles. Do they transfer infection agents from cell to cell?…”

Section: Tgb Cooperating Systemmentioning

confidence: 99%

Cell-to-cell movement of three genera (+) ss RNA plant viruses

Otulak

Garbaczewska

2010

Acta Physiol Plant

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The current investigations of three genera plant virus cell-to-cell movement were presented. Viruses reveal different local transport strategies, but all of them are the results of virus factors-host components interactions. The Tobacco mosaic virus (TMV) does not require capsid protein for translocation through plasmodesmata but 30 K movement protein participates in this process. It was found direct or indirect TMV movement proteins host partners in Tobamovirus movement like: pectin methylesterase, movement protein binding 2C, chaperones or cytoskeleton components and endoplasmatic reticulum membranes. The Potex-and Potyvirus cell-to-cell movement is closely related to replication network. The PVX capsid protein and triple gene block protein system are responsible for efficient local transport. Potyviruses move through the plasmodesmata by involving viral encoded proteins but not specific movement proteins. While the Potyvirus is the biggest known plant virus genus, host components participating in or regulating directly its plasmodesmata-movement are still not clear.

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Triple gene block: modular design of a multifunctional machine for plant virus movement

Cited by 332 publications

References 181 publications

Mutation of a chloroplast-targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long-distance virus movement

Mutation of a chloroplast-targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long-distance virus movement

Molecular characterization and field survey of Iranian potato virus X isolates

Cell-to-cell movement of three genera (+) ss RNA plant viruses

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