Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels

Kurosawa, Kazu; Wewetzer, Sandra J.; Sinskey, Anthony J.

doi:10.4172/1948-5948.1000153

Cited by 40 publications

(25 citation statements)

References 36 publications

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Section: Promising Species For Industrial Production Of Fuels and Chesupporting

confidence: 88%

“…Overall, the total TAG titre attained on OMSW fibre (15.2 ± 1.1 g/L) corresponds well with work by Kurosawa et al [64] wherein MITXM-61 grown on corn stover hydrolysate produced a 15.9 g/L TAG. However, the overall productivity of MITXM-61 was significantly greater on OMSW fibre (0.21 ± 0.02 g/L/h) compared to corn stover (0.13 g/L/h) [64] due to a shorter lag phase. There was a 48 h lag phase before growth commenced on corn stover [64], whereas in the two fermentation trial carried out with R. opacus on OMSW fibre, the lag phase only lasted ~ 12 h ( Fig.…”

Section: Promising Species For Industrial Production Of Fuels and Chesupporting

confidence: 88%

“…TAG is a promising precursor for the production of biodiesel and aviation fuel, but can also be derivatised to produce a range of valuable chemicals including polymers and surfactants [61][62][63]. R. opacus MITXM-61 was engineered by Kurosawa et al [64] for simultaneous utilisation of d-glucose and d-xylose in lignocellulosic hydrolysates. In this study R. opacus MITXM-61 was the top performing strain in terms of sugar utilisation.…”

Section: Characterising Growth Of Eight Microbial Species On Omsw Fibmentioning

confidence: 99%

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Robust microorganisms for biofuel and chemical production from municipal solid waste

et al. 2020

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Background: Worldwide 3.4 billion tonnes of municipal solid waste (MSW) will be produced annually by 2050, however, current approaches to MSW management predominantly involve unsustainable practices like landfilling and incineration. The organic fraction of MSW (OMSW) typically comprises ~ 50% lignocellulose-rich material but is underexplored as a biomanufacturing feedstock due to its highly inconsistent and heterogeneous composition. This study sought to overcome the limitations associated with studying MSW-derived feedstocks by using OMSW produced from a realistic and reproducible MSW mixture on a commercial autoclave system. The resulting OMSW fibre was enzymatically hydrolysed and used to screen diverse microorganisms of biotechnological interest to identify robust species capable of fermenting this complex feedstock. Results:The autoclave pre-treated OMSW fibre contained a polysaccharide fraction comprising 38% cellulose and 4% hemicellulose. Enzymatic hydrolysate of OMSW fibre was high in d-glucose (5.5% w/v) and d-xylose (1.8%w/v) but deficient in nitrogen and phosphate. Although relatively low levels of levulinic acid (30 mM) and vanillin (2 mM) were detected and furfural and 5-hydroxymethylfurfural were absent, the hydrolysate contained an abundance of potentially toxic metals (0.6% w/v). Hydrolysate supplemented with 1% yeast extract to alleviate nutrient limitation was used in a substrate-oriented shake-flask screen with eight biotechnologically useful microorganisms (Clostridium saccharoperbutylacetonicum, Escherichia coli, Geobacillus thermoglucosidasius, Pseudomonas putida, Rhodococcus opacus, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Zymomonas mobilis). Each species' growth and productivity were characterised and three species were identified that robustly and efficiently fermented OMSW fibre hydrolysate without significant substrate inhibition: Z. mobilis, S. cerevisiae and R. opacus, respectively produced product to 69%, 70% and 72% of the maximum theoretical fermentation yield and could theoretically produce 136 kg and 139 kg of ethanol and 91 kg of triacylglycerol (TAG) per tonne of OMSW. Conclusions: Developing an integrated biorefinery around MSW has the potential to significantly alleviate the environmental burden of current waste management practices. Substrate-oriented screening of a representative and reproducible OMSW-derived fibre identified microorganisms intrinsically suited to growth on OMSW hydrolysates. These species are promising candidates for developing an MSW biorefining platform and provide a foundation for future studies aiming to valorise this underexplored feedstock. © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third part...

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Section: Promising Species For Industrial Production Of Fuels and Chesupporting

confidence: 88%

Section: Promising Species For Industrial Production Of Fuels and Chesupporting

confidence: 88%

Section: Characterising Growth Of Eight Microbial Species On Omsw Fibmentioning

confidence: 99%

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Robust microorganisms for biofuel and chemical production from municipal solid waste

et al. 2020

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“…opacus MITXM-61 was constructed in our previous study, and deposited with the ATCC patent depository (PTA-12196) (Kurosawa et al, 2014). The strain was preserved in 20% (w/v) glycerol at À80°C.…”

Section: Strain and Mediamentioning

confidence: 99%

“…Rhodococcus opacus MITXM-61 is an oleaginous bacterium capable of producing large quantities of lipids on high concentrations of glucose and xylose present in lignocellulosic hydrolysates (Fei et al, 2015;Kurosawa et al, 2014). The lipids are stored intracellularly as lipid bodies and are mainly composed of TAGs, consisting primarily of C16 and C18 series of long chain fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Lignocellulose-derived inhibitors improve lipid extraction from wet Rhodococcus opacus cells

Kurosawa

Debono

Sinskey

2015

Bioresource Technology

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Extracting lipids from oleaginous microbial cells in a cost effective and environmentally compatible manner remains a critical challenge in developing manufacturing paradigms for advanced liquid biofuels. In this study, a new approach using microbial growth inhibitors from lignocellulose-derived feedstocks was used to extract lipids efficiently from wet cell mass of the oleaginous bacterium Rhodococcus opacus MITXM-61. Nine common lignocellulose-derived inhibitors for treatment of cells prior to solvent extraction were used and evaluated for their efficiency of lipid extraction from the cells. When the inhibitors were individually examined, formic acid and furfural showed the highest extraction efficiency of lipids from wet cell mass. Multiple extractions of lipids with methanol from wet cell mass pretreated with combined common inhibitors or hardwood hydrolysate comprising lignocellulose-derived inhibitors resulted in lipid recovery of greater than 85% of total lipids, a 1.7-fold increase of lipid extraction as compared to those in the absence of the inhibitors.

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Current Developments in Industrial Fermentation Processes

Niakousari

Razmjooei

Nejadmansouri

et al. 2021

Fermentation Processes

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Triacylglycerol Production from Corn Stover Using a Xylose-Fermenting Rhodococcus opacus Strain for Lignocellulosic Biofuels

Cited by 40 publications

References 36 publications

Robust microorganisms for biofuel and chemical production from municipal solid waste

Robust microorganisms for biofuel and chemical production from municipal solid waste

Lignocellulose-derived inhibitors improve lipid extraction from wet Rhodococcus opacus cells

Current Developments in Industrial Fermentation Processes

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