Treponema spp., the dominant pathogen in the lesion of bovine digital dermatitis and its characterization in dairy cattle

Mamuad, Lovelia L.; Seo, Byoung Joo; Faruk, Md. Shohel Al; Espiritu, Hector M.; Jin, Su Jeong; Kim, Won-Il; Lee, Sang-Suk; Cho, Yong-Il

doi:10.1016/j.vetmic.2020.108696

Cited by 17 publications

(22 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In total, 66 samples pre-assessed from our previous research [ 16 ] were used in this study. From these, one normal skin sample (Group A) and three BDD lesions (Group B) were subjected for analysis.…”

Section: Resultsmentioning

confidence: 99%

“…This study analyzed the diversity and phylogenetic relationship of the microbiome of BDD lesions compared to normal skin from the interdigital space of the cattle. From our previous study [ 16 ], we elucidated the relative abundance of Spirochetes, specifically Treponema spp. in BDD lesions and concluded that Treponema spp., was the dominant pathogen involved in BDD in Korea.…”

Section: Discussionmentioning

confidence: 99%

“…The abundance data previously presented by Mamuad et al, (2020) [ 16 ], to some extent, agree with the reports of Moreira et al, (2018) [ 14 ], Zinicola et al, (2015) [ 11 ], Beninger et al, (2016) [ 26 ], Krull et al, (2014) [ 13 ], and Nielsen et al, (2016) [ 12 ], that BDD is polybacterial, with Treponema spp., as the most abundant genus. However, the dominance and diversity of species under this genus still varies between reports from different geographical locations.…”

Section: Discussionmentioning

confidence: 99%

“…A total of 66 pre-assessed active BDD samples were subjected to detection of Treponema spp. by genus-specific PCR as described in the results of our previous study [ 16 ]. Since 100% of the active BDD lesions are positive in PCR, three samples were randomly selected for metagenomics sequencing.…”

Section: Methodsmentioning

confidence: 99%

“…In a recent report, Treponema spp. was established as the dominant pathogen in BDD lesions in Korea [ 16 ], but an emphasis on the plethora of bacteria that was observed is still lacking. Although the majority of studies considered that the main pathogen of BDD is Treponema spp., its polybacterial nature must also be considered in order to understand its pathogenesis [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Microbiome Shift, Diversity, and Overabundance of Opportunistic Pathogens in Bovine Digital Dermatitis Revealed by 16S rRNA Amplicon Sequencing

Espiritu

Mamuad

Kim

et al. 2020

Animals

Self Cite

View full text Add to dashboard Cite

This study analyzed the diversity and phylogenetic relationship of the microbiome of bovine digital dermatitis (BDD) lesions and normal skin from cattle foot by using 16S rRNA amplicon sequencing. Three BDD samples and a normal skin sample were pre-assessed for analysis. The Illumina Miseq platform was used for sequencing and sequences were assembled and were categorized to operational taxonomic units (OTUs) based on similarity, then the core microbiome was visualized. The phylogeny was inferred using MEGA7 (Molecular evolutionary genetics analysis version 7.0). A total of 129 and 185 OTUs were uniquely observed in normal and in BDD samples, respectively. Of the 47 shared OTUs, 15 species presented increased abundance in BDD. In BDD and normal samples, Spirochetes and Proteobacteria showed the most abundant phyla, respectively, suggesting the close association of observed species in each sample group. The phylogeny revealed the evolutionary relationship of OTUs and the Euclidean distance suggested a high sequence divergence between OTUs. We concluded that a shift in the microbiome leads to richer diversity in BDD lesions, and the overabundance of opportunistic pathogens and its synergistic relationship with commensal bacteria could serve as factors in disease development. The influence of these factors should be thoroughly investigated in future studies to provide deeper insights on the pathogenesis of BDD.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Microbiome Shift, Diversity, and Overabundance of Opportunistic Pathogens in Bovine Digital Dermatitis Revealed by 16S rRNA Amplicon Sequencing

Espiritu

Mamuad

Kim

et al. 2020

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

A metagenomics approach to characterize the footrot microbiome in Merino sheep

Usié

Leão

Gaspar

et al. 2023

Veterinary Microbiology

View full text Add to dashboard Cite

Applying PICRUSt and 16S rRNA functional characterisation to predicting co-digestion strategies of various animal manures for biogas production

Ijoma

Nkuna

Mutungwazi

et al. 2021

Sci Rep

View full text Add to dashboard Cite

An estimated 25 million tons of animal manure is produced globally every year, causing considerable impact to the environment. These impacts can be managed through the use of anaerobic digestion (AD) This process achieves waste degradation through enzymatic activity, the efficiency of the AD process is directly related to microorganisms that produce these enzymes. Biomethane potential (BMP) assays remain the standard theoretical framework to pre-determine biogas yield and have been used to determine the feasibility of substrates or their combination for biogas production. However, an integrated approach that combines substrate choice and co-digestion would provide an improvement to the current predictive models. PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) addresses the limitations of assays in this regard. In this paper, the biochemical functions of horse, cow, and pig manures are predicted. A total of 135 predicted KEGG Orthologies (KOs) showed amino acids, carbohydrate, energy, lipid, and xenobiotic metabolisms in all the samples. Linear discriminant analysis (LDA) combined with the effect size measurements (LEfSe), showed that fructose, mannose, amino acid and nucleotide sugar, phosphotransferase (PST) as well as starch and sucrose metabolisms were significantly higher in horse manure samples. 36 of the KOs were related to the acidogenesis and/or acetogenesis AD stages. Extended bar plots showed that 11 significant predictions were observed for horse-cow, while 5 were predicted for horse-pig and for cow-pig manures. Based on these predictions, the AD process can be enhanced through co-digestion strategies that takes into account the predicted metabolic contributions of the manure samples. The results supported the BMP calculations for the samples in this study. Biogas yields can be improved if this combined approach is employed in routine analysis before co-digesting different substrates.

show abstract

Treponema spp., the dominant pathogen in the lesion of bovine digital dermatitis and its characterization in dairy cattle

Cited by 17 publications

References 23 publications

Microbiome Shift, Diversity, and Overabundance of Opportunistic Pathogens in Bovine Digital Dermatitis Revealed by 16S rRNA Amplicon Sequencing

Microbiome Shift, Diversity, and Overabundance of Opportunistic Pathogens in Bovine Digital Dermatitis Revealed by 16S rRNA Amplicon Sequencing

A metagenomics approach to characterize the footrot microbiome in Merino sheep

Applying PICRUSt and 16S rRNA functional characterisation to predicting co-digestion strategies of various animal manures for biogas production

Contact Info

Product

Resources

About