2012
DOI: 10.1007/s10616-012-9431-8 View full text |Buy / Rent full text
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Abstract: The identification of egg extracts with the ability to maintain and enhance the survival and differentiation of cells would be widely useful in cellular biology research. In this study, we compared the different abilities of spleen cells to survive and differentiate in vivo after permeabilization by five different types of egg extracts. Five types of egg extracts were prepared. The spleen cells from male GFP-transgenic mice were permeabilized by the extracts for 30 min, cultured for 12 days, and then transfuse… Show more

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“…Chicken egg white contains an internal signal sequence of mature ovalbumin comprising residues 229-276 that can be recovered as a tryptic fragment (McReynolds et al 1978;Lingappa et al 1979). Our previous studies have found that chicken egg white can function in the maintenance of cell activity (Ruan et al 2012), and our present study found that components in chicken egg white extract smaller than 3 kDa in size can promote cell proliferation and survival. This discovery may identify a new and convenient additive for cell culture media that promotes cell growth and proliferation.…”
Section: Introductionsupporting
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“…Chicken egg white contains an internal signal sequence of mature ovalbumin comprising residues 229-276 that can be recovered as a tryptic fragment (McReynolds et al 1978;Lingappa et al 1979). Our previous studies have found that chicken egg white can function in the maintenance of cell activity (Ruan et al 2012), and our present study found that components in chicken egg white extract smaller than 3 kDa in size can promote cell proliferation and survival. This discovery may identify a new and convenient additive for cell culture media that promotes cell growth and proliferation.…”
Section: Introductionsupporting
“…Studies have shown that mammalian egg cell and Xenopus egg cell extracts can reprogram somatic cells [6,7]. Our previous research showed that chicken egg extract can also reprogram somatic cells [5,9]. The chicken egg is the largest egg cell, and its ability to reprogram somatic cells will thus advance cell biological research.…”
Section: Plos Onementioning
“…Therefore, chicken egg white extract has the capacity to induce stemness in PBMCs. In our previous research, we found that chicken egg white extract can induce the reverse differentiation of somatic cells into multipotent stem cells [5,9].…”
Section: Introductionmentioning
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“… 13 , 14 More importantly, extracts and proteins from EW support cell growth and proliferation. 15 , 16 Therefore, EW can serve as a suitable material to culture cells in the 3-D format. Furthermore, the nontoxic property, biocompatibility, and biodegradability of EW are added advantages to use it as a 3-D cell culture platform.…”
Section: Introductionmentioning