“…The commencement of semi-continuous addition of liquid brewery waste 22 days later (Figure 1), in the form of 34 ml added daily, was followed by a small decrease in both total and filtered Zn concentrations in the effluent water for a period of approximately 7 days, but effluent Zn concentrations subsequently showed a gradual increase and treatment efficiency reduced to 47% (total Zn) and 70% (filtered Zn), which was significantly lower than that prior to carbon additions (Figure 1). Again, it is not certain why this occurred, though it is possible that the volume of carbon added was still too great, particularly given the relatively low influent sulfate concentration in the Rampgill mine water, compared to the higher influent sulfate concentration used in successful laboratory trials adding similar volumes of carbon (Mayes et al, 2011). Martins et al (2011) observed the same phenomenon when doubling the amount of carbon, in the form of wine wastes, added to laboratory columns, which resulted in an instant deterioration in performance.…”