Treatment of non-healing wounds with autologous bone marrow cells, platelets, fibrin glue and collagen matrix

Ravari, Hassan; Hamidi-Almadari, Daryoush; Salimifar, Mohsen; Bonakdaran, Shokoufeh; Parizadeh, Mohammad Reza; Koliakos, George

doi:10.3109/14653249.2011.553594

Cited by 81 publications

(43 citation statements)

References 34 publications

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“…According to the study by Ravari et al (41), it was hypothesized that for those patients who did not respond to the PFRP therapy, considering the pathological factors associated with the phenotypically altered and/or senescent mesenchymal cells in the wound, stem cell therapy should be considered as a second approach for the treatment of recalcitrant wounds. This hypothesis requires further study.…”

Section: Discussionmentioning

confidence: 99%

Treatment of life-threatening wounds with a combination of allogenic platelet-rich plasma, fibrin glue and collagen matrix, and a literature review

Asmar

Alamdari

Rahimi

et al. 2014

Experimental and Therapeutic Medicine

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Currently there is no ideal procedure for the treatment of recalcitrant ulcers that are unresponsive to the majority of common treatments. However, several novel approaches have been proposed, including bone marrow stem cells, platelets, fibrin glue and collagen matrix. For the first approach treatment of a chronic wound, a non-invasive method is highly desirable. The present study was undertaken with the aim of evaluating the effect of a combination of platelets, fibrin glue and collagen matrix (PFC) in one treatment. A total of ten patients with aggressive, refractory, life-threatening wounds were recruited for the study and their treatment effects were evaluated. Initially, the ulcers were extensively debrided, measured and photographed at weekly intervals. The PFC combination was applied topically to the wound every two days. Following treatment, the wound was completely closed in nine patients and was markedly reduced in the other patient. The mean 100% healing time for the nine patients was 11.3±5.22 weeks. There was no evidence of local or systemic complications or any abnormal tissue formation, keloid or hypertrophic scarring. Therefore, the results of the present study indicate that in the first approach, the combination of PFC components may be used safely in order to synergize the effect of chronic wound healing.

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Section: Discussionmentioning

confidence: 99%

Treatment of life-threatening wounds with a combination of allogenic platelet-rich plasma, fibrin glue and collagen matrix, and a literature review

Asmar

Alamdari

Rahimi

et al. 2014

Experimental and Therapeutic Medicine

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“…Application of autologous BM-MSCs in a fibrin spray system has been shown to induce closure of wounds in diabetic mice and appears to be a safe procedure for the treatment of cutaneous wounds in human [94]. Transplantation of BM-MSCs with platelets, fibrin glue and bone marrow-impregnated collagen matrix, appears to aid healing of chronic refractory wounds in diabetic patients [95].…”

Section: Figurementioning

confidence: 98%

Autologous stem cells for personalised medicine

Prasongchean¹,

Ferretti²

2012

New Biotechnology

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Increasing understanding of stem cell biology, the ability to reprogramme differentiated cells to a pluripotent state and evidence of multipotency in certain adult somatic stem cells has opened the door to exciting therapeutic advances as well as a great deal of regulatory and ethical issues. Benefits will come from the possibility of modelling human diseases and develop individualised therapies, and from their use in transplantation and bioengineering. The use of autologous stem cells is highly desirable, as it avoids the problem of tissue rejection, and also reduces ethical and regulatory issues. Identification of the most appropriate cell sources for different potential applications, development of appropriate clinical grade methodologies and large scale well controlled clinical trials will be essential to assess safety and value of cell based therapies, which have been generating much hope, but are by and large not yet close to becoming standard clinical practice. We briefly discuss stem cells in the context of tissue repair and regenerative medicine, with a focus on individualised clinical approaches, and give examples of sources of autologous cells with potential for clinical intervention.

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“…Collagen is well known protein that had wide application in medical field as drug delivery system and tissue engineering (Lee et al, 2001). Collagen also served as matrix to transport cytokine, drug and even cultured skin cells to wound (Hart et al, 2002;Lee et al, 2001;Ravari et al, 2011;Varga et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Efforts were spent to develop collagen as scaffold to aid in wound healing, facilitate cytokine transportation to reduce inflammation and promote proliferation stage, in hope to speed up healing process (Enoch & Leaper 2008;Hart et al, 2002;Ravari et al, 2011;Varga et al, 2014;Williamson & Harding 2004;Yan et al, 2010). Commonly, collagen used in medical field was extracted from bovine source (Pati et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Clarias batrachus collagen extract increases fibroblast cell adhesion, migration and proliferation

Leong

Sahalan²,

Tan

et al. 2015

J App Pharm Sci

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Collagen is an extracellular matrix protein with great importance in biomedical application. The search for collagen from various sources is intensified especially from marine source. This study was carried out to extract collagen from a Malaysian local fresh water fish, Clarias batrachus and characterized its biomedical potential In vitro. Collagen was extracted from C. batrachus skin using acetic acid method and identified using SDS-PAGE. MTT assay was performed to determine the effect of coated collagen on cell adhesion and proliferation of L929 skin fibroblast cells. Additionally, scratch assay was performed to examine the effect of collagen coating on fibroblast cell migration. Result showed that collagen extracted from C. batrachus was made up of collagen type I, which consists of two α chains (α-1 and α-2) and β chain. At 100 µg/cm 2 density, collagen coating significantly increased fibroblast cell adhesion, proliferation and migration compared to negative control (p< 0.05). As a conclusion, collagen extracted from C. batrachus increased cell adhesion, proliferation and migration of fibroblast cells and has potential to be used as an alternative source of collagen.

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Treatment of non-healing wounds with autologous bone marrow cells, platelets, fibrin glue and collagen matrix

Cited by 81 publications

References 34 publications

Treatment of life-threatening wounds with a combination of allogenic platelet-rich plasma, fibrin glue and collagen matrix, and a literature review

Treatment of life-threatening wounds with a combination of allogenic platelet-rich plasma, fibrin glue and collagen matrix, and a literature review

Autologous stem cells for personalised medicine

Clarias batrachus collagen extract increases fibroblast cell adhesion, migration and proliferation

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