Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells

Monteiro, Betânia Souza; Neto, Napoleão Martins Argôlo; Nardi, Nance Beyer; Chagastelles, Pedro César; Carvalho, Pablo Herthel de; Bonfa, Laila P; Filgueiras, Roberto; Reis, Amanda S; Carlo, Ricardo Junqueira Del

doi:10.1590/s0001-37652012000300026

Cited by 10 publications

(10 citation statements)

References 25 publications

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“…These immunosuppressive properties have already been described in vitro by Nauta and Fibbe (3), Kode et al (9), and Zhao et al (11), and tested in vivo by Hernigou et al (12) as autologous transplants, Monteiro et al (6) as isogeneic transplants, and Chuang In this study, it was also demonstrated that xenotransplantation of rat bone marrow-derived mesenchymal stem cells efficiently promoted bone repair of gaps created in the radius of rabbits, which were not immunosuppressed over the study period. Radiographic findings showed that the contribution of the cells in the bone repair process are similar to those reported by Shoji et al (15) when treating femoral fractures in rats with human adipose-tissue derived mesenchymal stem cells.…”

Section: Discussionmentioning

confidence: 83%

Clinical and radiographic characterization of xenotransplantation of rat bonemarrow-derived mesenchymal stem cells for repair of radial defects of rabbit

Monteiro¹,

Favarato²,

Carvalho³

et al. 2015

Turk J Vet Anim Sci

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IntroductionMesenchymal stem cells (MSCs) are defined as a population of multipotent cells able to differentiate and produce any cell type needed in a repair process, such as osteoblasts, chondroblasts, neurons, epithelial cells, and cardiac cells (1,2).This cell type has become the focus of numerous studies worldwide for providing clinically promising perspectives for cell therapy and also for its immunomodulatory potential (3,4), although the mechanisms of immunosuppression on inflammatory response and the mechanisms of transplant rejection are not fully elucidated (5).Recent studies have described the use of allogeneic and autologous MSCs for the repair of various tissues (4,6). However, there is little research involving xenotransplantation in animals and most of them only evaluated the cellular interaction in vitro between MSCs and T lymphocytes.Because of the great therapeutic potential of MSCs, in addition to the persistent doubts about their immunosuppressive capacity in vivo, further studies are needed to investigate the real potential of xenogenic transplantation using these cells for tissue repair in animals. Therefore, this study evaluated clinical and radiographic aspects of xenogenic transplantation of rat bone marrow-derived MSCs for the repair of radial bone defects created in rabbits. Materials and methodsThis study was approved by the Institutional Animal Care Committee (protocol 97/2010). Cellular cultureA total of five male, 4-week old Wistar rats were euthanized using anesthetic overdose. The animals were immersed in alcohol 70° to ensure antisepsis for cell collection and were taken to the laminar flow cabinet. The femurs were disarticulated and removed aseptically. The distal epiphyses were cut and the medullary canal was flushed with Dulbecco's modified Eagle's medium (DMEM, Gibco, Grand Island, NY, USA) with low glucose, containing 10% fetal bovine serum (FBS) (Gibco), 50.0 mg L -1 gentamicin,

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Section: Discussionmentioning

confidence: 83%

Clinical and radiographic characterization of xenotransplantation of rat bonemarrow-derived mesenchymal stem cells for repair of radial defects of rabbit

Monteiro¹,

Favarato²,

Carvalho³

et al. 2015

Turk J Vet Anim Sci

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“…These results suggest that soluble molecules released by the stem cells, upon contact with the injured tissue, triggers a series of beneficial cellular events in favor of tissue repair capable of minimizing vascular injury by regulating the capillary hydrostatic pressure and shortening edema formation (Nauta & Fibbe 2007, Jiang et al 2011, Monteiro et al 2012.…”

Section: Discussionmentioning

confidence: 96%

Allogenic mesenchymal stem cell intravenous infusion in reparation of mild intestinal ischemia/reperfusion injury in New Zealand rabbits

et al. 2018

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The present study aimed to evaluate the efficacy of mesenchymal stem cell (MSC) infusion, derived from adipose tissue, on reduction of local and remote tissue damage caused by the event of experimental intestinal I/R in New Zealand breed rabbits. For obtaining, characterization, and cultivation of MSC derived from adipose tissue (MSC-Adp), 3 juvenile animals (four months old) were used. The cells were considered to be viable for therapy after the fourth passage (in vitro phase). For the in vivo stage, 24 young adult animals (six months old) were used, weighing approximately 3.5 kg, in which were randomly divided into two groups, called: IR treated with MSC (I2H/R5H MSC 3D; I2H/R5H MSC 7D); IR treated with PBS (I2H/R5H PBS 3D; I2H/R5H PBS 7D). The animals were anesthetized and submitted to pre-retro-umbilical midline celiotomy. The extramural peri-intestinal marginal artery was located and clamped (predetermined and standardized region) with the aid of a vascular clip, promoting a 2 hour blood flow interruption. After this period, blood flow was reestablished, inhalatory anesthesia was suspended, and the animals awaken. After 5 hours of reperfusion, the treatments were performed by intravenous infusion according to the experimental groups. The animals were evaluated 72 hours and seven days after the treatment as for the macroscopic appearance (color and peristaltism) of the jejunal segment, and by histological evaluation of the ischemic segment for the presence or absence of destruction of the intestinal mucosa, edema, bleeding, dilation of lymph vessels, and presence of polymorphonuclear inflammatory cells, both in the mucosa and submucosa. The observed results revealed that the groups treated with MSC-Adp obtained smaller mucosal and submucosal lesions when compared to the groups treated with PBS. Also, MSC-Adp treated groups obtained controlled inflammatory response and higher mitotic rate, outcomes related to the therapeutic potential of MSC. Infusion of stem cells attenuated the lesions caused by intestinal I/R in both MSC groups when compared to the group treated with PBS.

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“…In this study, the clinical findings were the presence of eye discharge and areas of hypotrichosis in animals after 14 days of dexamethasone application. MSC protocol used in this experiment was based on similar studies that evaluated the therapeutic potential of this cell group in skin wounds (WU et al, 2007;MONTEIRO et al, 2012). A recent study described the allogeneic and autologous utilization of MSC in the repair of many tissues (MONTEIRO et al, 2010;MONTEIRO et al, 2012); however, the ability of these cells to restore the dermal tissue is still unclear.…”

Section: Discussionmentioning

confidence: 99%

“…MSC protocol used in this experiment was based on similar studies that evaluated the therapeutic potential of this cell group in skin wounds (WU et al, 2007;MONTEIRO et al, 2012). A recent study described the allogeneic and autologous utilization of MSC in the repair of many tissues (MONTEIRO et al, 2010;MONTEIRO et al, 2012); however, the ability of these cells to restore the dermal tissue is still unclear. Even not having performed the in situ stem cell transplantation directly on the skin tissue and having chosen the intravenous application (lateral tail vein), it was reported in the MSCDG group a greater amount of collagen fibers, which, at day 15, were thicker than in the other groups (MONTEIRO et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Isolation, characterization and expansion of MSC were carried out according to the methodology described by MONTEIRO et al (2012). An aliquot of cells from the fourth passage was characterized by flow cytometric analysis of the expression of CD 34, CD 45, CD 90 and CD 54 on the surface of cells using a FACScan flow cytometer and CellQuest ® software, yielding 30,000 events per sample tested.…”

Section: Cell Culturementioning

confidence: 99%

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Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats

et al. 2016

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To evaluate the therapeutic contribution of MSC intravenous infusion to surgical wound healing in dexamethasone-immunosuppressed rats, thirty-five rats were randomly divided into 2 groups: in the Control Group (CG), five rats received normal saline as 0.2ml subcutaneous (SC) injections every 24 hours, for 30 consecutive days and, in the Dexamethasone Group (DG), 30 rats were given 0.2mL subcutaneous dexamethasone (0.1mg kg-1) every 24 hours, for 30 consecutive days. After 30 days, all rats underwent surgery to create an experimental skin wound. The 30 animals of the DG group were divided into two equal groups, which received different treatments: the dexamethasone group (DG) received a single application of 0.5ml normal saline, via the intravenous route (IV), 48 hours after wound creation; and the Mesenchymal Stem Cells Dexamethasone group (MSCDG) received MSC transplantation at a concentration of 9x106 cells in a single IV application, 48 hours after wound creation. The surgical wounds of CG rats closed on average 14.75 days after creation and DG rats had wounds closed within 22 days; whereas, the surgical wounds of MSCDG rats were closed in 14 days. MSC infusion in dexamethasone-immunosuppressed patients contributed positively to epithelial healing in less time.

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Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells

Cited by 10 publications

References 25 publications

Clinical and radiographic characterization of xenotransplantation of rat bonemarrow-derived mesenchymal stem cells for repair of radial defects of rabbit

Clinical and radiographic characterization of xenotransplantation of rat bonemarrow-derived mesenchymal stem cells for repair of radial defects of rabbit

Allogenic mesenchymal stem cell intravenous infusion in reparation of mild intestinal ischemia/reperfusion injury in New Zealand rabbits

Mesenchymal stem cell infusion on skin wound healing of dexamethasone immunosuppressed wistar rats

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