Treatment of chancroid. A comparison of tetracycline and sulfisoxazole

Kerber, R. E.

doi:10.1001/archderm.100.5.604

Cited by 14 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several reports have described clinical resistance to sulfonamides; however, in vitro susceptibility studies were not performed (63,78,101). Sulfonamide resistance in H. ducreyi is prevalent throughout the world (Table 8).…”

Section: Genetics and Mechanisms Of Antimicrobial Resistancementioning

confidence: 99%

“…They have been reported to be effective therapy for chancroid (11,172). Recent studies (Table 8) (78,84) have reported clinical resistance and in vitro resistance of H. ducreyi to the tetracyclines. Tetracycline-resistant strains have been found in every geographic area surveyed, with the percentage of resistant strains varying from 16 to 100% (8).…”

Section: Tetracyclinementioning

confidence: 99%

See 1 more Smart Citation

Chancroid and Haemophilus ducreyi

1989

View full text Add to dashboard Cite

Haemophilus ducreyi is the causative agent of chancroid, one of the genital ulcerative diseases. H. ducreyi is the major cause of genital ulcer disease in Africa and Southeast Asia and is of increasing concern in the United States. Definitive diagnosis of chancroid requires the isolation and identification of H. ducreyi, but isolation of this organism is difficult and the available medium is not optimal for all strains. Fluorescent antibody and serologic tests are of limited value. In general, our knowledge of this organism is rather limited, and indeed, recent studies have questioned the placement of H. ducreyi in the genus Haemophilus. H. ducreyi has relatively few biochemical activities, and epidemiologic studies are limited because there are limited phenotypic markers available for strain typing. Specific virulence factors of H. ducreyi have yet to be identified. Antimicrobial resistance in H. ducreyi is of special concern, as this organism has acquired both gram-negative and gram-positive resistance determinants. In addition, some of these determinants can be mobilized and transferred to other Haemophilus species or to Neisseria gonorrhoeae.

show abstract

Section: Genetics and Mechanisms Of Antimicrobial Resistancementioning

confidence: 99%

Section: Tetracyclinementioning

confidence: 99%

Chancroid and Haemophilus ducreyi

1989

View full text Add to dashboard Cite

show abstract

“…Research conducted among soldiers serving in the U.S. Forces assigned to Vietnam revealed that chancroid was the second most frequently reported venereal disease, the first one being gonorrhea (Kerber et al, 1969). Another venereal infection prevalent in hot climate areas is lymphogranuloma venereum.…”

Section: Vietnam Warmentioning

confidence: 99%

Sexually Transmitted Infections Among Army Personnel in the Military Environment

Korzeniewski¹

2012

Sexually Transmitted Infections

View full text Add to dashboard Cite

“…Recent studies, however, have reported clinical resistance and in vitro resistance of the causative organism, Haemophilus ducreyi, to the tetracyclines (10,15,16). In our laboratory, in vitro studies with a large collection of strains have shown over 90% of the isolates from selected geographic areas to be resistant to tetracyclines.…”

mentioning

confidence: 90%

Plasmid-mediated tetracycline resistance in Haemophilus ducreyi

Albritton¹,

Maclean²,

Slaney³

et al. 1984

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Clinical isolates of Haemophilus ducreyi were shown to be resistant to tetracycline. Resistance was associated in some strains with a 30-megadalton plasmid capable of transferring resistance in conjugative matings with other strains of H. ducreyi and other species of Haemophilus. Restriction endonuclease digestion patterns suggest a relationship between H. ducreyi plasmids and other tetracycline resistance plasmids in Haemophilus. The presence of plasmid-mediated resistance to the tetracyclines limits the use of these agents for the treatment of chancroid.Tetracyclines have been reported as effective therapy for chancroid (4,24). Recent studies, however, have reported clinical resistance and in vitro resistance of the causative organism, Haemophilus ducreyi, to the tetracyclines (10,15,16). In our laboratory, in vitro studies with a large collection of strains have shown over 90% of the isolates from selected geographic areas to be resistant to tetracyclines.Since tetracycline resistance has been shown previously to be plasmid mediated in other species of Haemophilus (14), and since plasmid-mediated resistance to ampicillin (5-7) and sulfonamides (3) has been previously demonstrated in H. ducreyi, we looked for plasmid-mediated resistance to tetracyclines in this species.We report here the demonstration and preliminary characterization of a conjugative plasmid encoding tetracycline resistance in clinical isolates of H. ducreyi. MATERIALS AND METHODSBacterial strains and plasmids. Bacterial strains and plasmids named in this study are listed in Table 1. The remainder of the strains were clinical isolates of H. ducreyi.Media. Strains of H. ducreyi were routinely grown on GC agar base (GIBCO Diagnostics, Madison, Wis.) supplemented with 1% hemoglobin and 1% CVA enrichment (GIBCO).MICs were determined by agar dilution as described previously (11).Plasmid DNA. Strains were screened for the presence of plasmids as described by Meyers et al. (19) and previously reported (3, 6). Plasmid DNA for restriction digestion was prepared by a modification (8) nisms from 4 ml of these suspensions were filtered onto 0.45-,um polycarbonate filters. After overnight incubation on the surface of a chocolate agar plate, the organisms were resuspended and spread over the surface of chocolate agar containing tetracycline (4 ,ug/ml) and streptomycin (1,000 ,ug/ml). The clones isolated were screened for plasmid DNA, and their antibiotic resistance was confirmed by disk diffusion testing. RESULTSThe distribution of MICs for 35 strains of H. ducreyi is shown in Fig. 1. Resistant strains were defined as those having an MIC 2 4 ,ug/ml. Tetracycline-resistant strains were found in every geographical area surveyed, with the percentage of resistant strains varying from 16 to 100 (Table 2).Forty-one tetracycline-resistant strains were screened for the presence of plasmids. All but one strain contained plasmids similar in size to one of three previously described nonconjugative plasmids of molecular mass 4.9, 5.7, and 7.0 megadaltons (Mdal) (3, ...

show abstract

Treatment of chancroid. A comparison of tetracycline and sulfisoxazole

Cited by 14 publications

References 0 publications

Chancroid and Haemophilus ducreyi

Chancroid and Haemophilus ducreyi

Sexually Transmitted Infections Among Army Personnel in the Military Environment

Plasmid-mediated tetracycline resistance in Haemophilus ducreyi

Contact Info

Product

Resources

About