Transplantation of SNAP-treated adipose tissue-derived stem cells improves cardiac function and induces neovascularization after myocardium infarct in rats

Berardi, Gel R.M.; Rebelatto, Carmen K.; Tavares, Heloísa F.; Ingberman, Max; Shigunov, Patrícia; Barchiki, Fabiane; Aguiar, Alessandra Melo de; Miyague, Nelson Itiro; Francisco, Júlio César; Correa, Alejandro; Senegaglia, Alexandra Cristina; Suss, Paula Hansen; Moutinho, J.; Sotomaior, Vanessa Santos; Nakao, Lia S.; Brofman, Paulo

doi:10.1016/j.yexmp.2010.11.005

Cited by 20 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pharmacological treatment of ADSCs, with rosuvastatin [51], ghrelin [52], T0901317 [53], exendin-4 [54], S-nitroso- N -acetyl- d,l -penicillamine (SNAP) [55], and NapFF-NO (FFGGG, and b-galactose caged nitric oxide) [56] etc., has increased the efficacy of ADSCs in promoting retention and the paracrine function of implanted cells. Rosuvastatin was used to promote the survival of ADSCs after transplantation into infarcted hearts.…”

Section: Introductionmentioning

confidence: 99%

A brief review: adipose-derived stem cells and their therapeutic potential in cardiovascular diseases

et al. 2017

View full text Add to dashboard Cite

Adipose-derived stem cells (ADSCs) are easily obtained and expanded, and have emerged as a novel source of adult stem cells for the treatment of cardiovascular diseases. These cells have been shown to have the capability of differentiating into cardiomyocytes, vascular smooth muscle cells, and endothelial cells. Furthermore, ADSCs secrete a series of paracrine factors to promote neovascularization, reduce apoptosis, and inhibit fibrosis, which contributes to cardiac regeneration. As a novel therapy in the regenerative field, ADSCs still face various limitations, such as low survival and engraftment. Thus, engineering and pharmacological studies have been conducted to solve these problems. Investigations have moved into phase I and II clinical trials examining the safety and efficacy of ADSCs in the setting of myocardial infarction. In this review, we discuss the differentiation and paracrine functions of ADSCs, the strategies promoting their therapeutic efficacy, and their clinical usage.

show abstract

Section: Introductionmentioning

confidence: 99%

A brief review: adipose-derived stem cells and their therapeutic potential in cardiovascular diseases

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Various research groups have studied its vasodilation effects [ 37 ] and regulation of the platelet aggregation in the past [ 38 ]. Meanwhile, NO molecules can also promote the expression of pro-angiogenic cytokines, favorable for angiogenesis [ 39 ]. Therefore, modulation in level of NO by cardiomyocytes in response to a vascular implant is of great interest to the engineers.…”

Section: Discussionmentioning

confidence: 99%

Topological control of nitric oxide secretion by tantalum oxide nanodot arrays

et al. 2015

View full text Add to dashboard Cite

BackgroundNitric oxide (NO) plays a very important role in the cardiovascular system as a major secondary messenger in signaling pathway. Its concentration regulates most of the important physiological indexes including the systemic blood pressure, blood flow, regional vascular tone and other cardiac functions. The effect of nanotopography on the NO secretion in cardiomyocytes has not been elucidated before. In this study, we report how the nanotopography can modulate the secretion profile of NO and attempt to elucidate the genetic pathways responsible for the same by using Tantalum Oxide nanodot arrays ranging from 10 to 200 nm. A series of nanodot arrays were fabricated with dot diameter ranging from 10 to 200 nm. Temporal NO release of cardiomyocytes was quantified when grown on different surfaces. Quantitative RT-PCR and Western blot were performed to verify the genetic pathways of NO release.ResultsAfter hours 24 of cell seeding, NO release was slowly enhanced by the increase of dot diameter from 10 nm up to 50 nm, mildly enhanced to a medium level at 100 nm, and increase rapidly to a high level at 200 nm. The temporal enhancement of NO release dropped dramatically on day 3. On day 5, a topology-dependent profile was established that maximized at 50 nm and dropped to control level at 200 nm. The NO releasing profile was closely associated with the expression patterns of genes associated with Endothelial nitric oxide synthase (eNOS) pathway [GPCR, PI3K, Akt, Bad, Bcl-2, NFκB(p65), eNOS], but less associated with Inducible nitric oxide synthase (iNOS) pathway (TNF-α, ILK, Akt, IκBα, NFκB, iNOS). Western blotting of Akt, eNOS, iNOS, and NFκB further validated that eNOS pathway was modulated by nanotopology.ConclusionsBased on the findings of the present study, 50, 100 nm can serve as the suitable nanotopography patterns for cardiac implant surface design. These two nanodot arrays promote NO secretion and can also promote the vascular smooth muscle relaxation. The results of this study can improve the heart stent design in the medical treatments.

show abstract

“…Yao and his group cultured adipose-derived MSCs in a hydrogel that can release NO molecule continuously and transplanted the hydrogel into murine myocardial infarction (MI) models, which achieved positive therapeutic effects (Yao et al, 2015). It has been widely confirmed that the protective effect of MSCs on MI is mainly achieved through the pro-angiogenic cytokines it secreted (Berardi et al, 2011). Similarly, Yao and co-workers discovered that NO hydrogel remarkably enhanced the paracrine and the VEGF secretion of MSCs.…”

Section: Small Bioactive Moleculesmentioning

confidence: 99%

Biophysical and Biochemical Cues of Biomaterials Guide Mesenchymal Stem Cell Behaviors

Liu

Zhang

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have been widely used in the fields of tissue engineering and regenerative medicine due to their self-renewal capabilities and multipotential differentiation assurance. However, capitalizing on specific factors to precisely guide MSC behaviors is the cornerstone of biomedical applications. Fortunately, several key biophysical and biochemical cues of biomaterials that can synergistically regulate cell behavior have paved the way for the development of cell-instructive biomaterials that serve as delivery vehicles for promoting MSC application prospects. Therefore, the identification of these cues in guiding MSC behavior, including cell migration, proliferation, and differentiation, may be of particular importance for better clinical performance. This review focuses on providing a comprehensive and systematic understanding of biophysical and biochemical cues, as well as the strategic engineering of these signals in current scaffold designs, and we believe that integrating biophysical and biochemical cues in next-generation biomaterials would potentially help functionally regulate MSCs for diverse applications in regenerative medicine and cell therapy in the future.

show abstract

Transplantation of SNAP-treated adipose tissue-derived stem cells improves cardiac function and induces neovascularization after myocardium infarct in rats

Cited by 20 publications

References 45 publications

A brief review: adipose-derived stem cells and their therapeutic potential in cardiovascular diseases

A brief review: adipose-derived stem cells and their therapeutic potential in cardiovascular diseases

Topological control of nitric oxide secretion by tantalum oxide nanodot arrays

Biophysical and Biochemical Cues of Biomaterials Guide Mesenchymal Stem Cell Behaviors

Contact Info

Product

Resources

About