Transplant of Oral Mucosal Epithelium to Rabbit Ocular Surface Wounds In Vivo

The combination of IFNα2b/5-FU inhibits proliferation and cell cycle of tumor cells in treating Ocular Surface Squamous Neoplasia

“…Due to the shortage of stable proliferating OSSN cell lines, lack of pathological tissue samples and challenges in primary cell culture, we employed human oral squamous cancer cell lines Cal27 which were easy to access for this research 46,47 . Although oral squamous cells resemble keratoconjunctival epithelium in physiological structures and biomarkers, they cannot fully represent proliferation characteristics of OSSN tumor cells [48][49][50] . In conclusion, this fundamental study testi ed the underlying reasons behind our clinical investigation, and the proved synergy between IFNα2b and 5-FU substantiated that topical combination chemotherapy would be a promising new therapeutic option for OSSN 18 .…”

The combination of IFNα2b/5-FU inhibits proliferation and cell cycle of tumor cells in treating Ocular Surface Squamous Neoplasia

“…Due to the shortage of stable proliferating OSSN cell lines, lack of pathological tissue samples and challenges in primary cell culture, we employed human oral squamous cancer cell lines Cal27 which were easy to access for this research 46,47 . Although oral squamous cells resemble keratoconjunctival epithelium in physiological structures and biomarkers, they cannot fully represent proliferation characteristics of OSSN tumor cells [48][49][50] . In conclusion, this fundamental study testi ed the underlying reasons behind our clinical investigation, and the proved synergy between IFNα2b and 5-FU substantiated that topical combination chemotherapy would be a promising new therapeutic option for OSSN 18 .…”

Simple oral mucosal epithelial transplantation in a rabbit model

“…Because severe LSCD accompanied by symblepharon is often bilateral, attention has been focused on autologous cell sources other than the corneal limbus, and the oral mucosa has been investigated as an option for ocular surface reconstruction 23 . Direct oral mucosal transplantation without culture after dispase treatment was reported to be successful in rabbits with partial corneal limbal scraping 24 . In that study, grafts were sutured over the wounds at the peripheral corneal-limbal junction.…”

Limbal Niche Cells: a Novel Feeder Cell for Autologous Cultivated Oral Mucosal Epithelial Transplantation

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The combination of IFNα2b/5-FU inhibits proliferation and cell cycle of tumor cells in treating Ocular Surface Squamous Neoplasia

“…Due to the shortage of stable proliferating OSSN cell lines, lack of pathological tissue samples and challenges in primary cell culture, we employed human oral squamous cancer cell lines Cal27 which were easy to access for this research 46,47 . Although oral squamous cells resemble keratoconjunctival epithelium in physiological structures and biomarkers, they cannot fully represent proliferation characteristics of OSSN tumor cells [48][49][50] . In conclusion, this fundamental study testi ed the underlying reasons behind our clinical investigation, and the proved synergy between IFNα2b and 5-FU substantiated that topical combination chemotherapy would be a promising new therapeutic option for OSSN 18 .…”

Simple oral mucosal epithelial transplantation in a rabbit model

“…Because severe LSCD accompanied by symblepharon is often bilateral, attention has been focused on autologous cell sources other than the corneal limbus, and the oral mucosa has been investigated as an option for ocular surface reconstruction 23 . Direct oral mucosal transplantation without culture after dispase treatment was reported to be successful in rabbits with partial corneal limbal scraping 24 . In that study, grafts were sutured over the wounds at the peripheral corneal-limbal junction.…”

Limbal Niche Cells: a Novel Feeder Cell for Autologous Cultivated Oral Mucosal Epithelial Transplantation

“……”

The combination of IFNα2b/5-FU inhibits proliferation and cell cycle of tumor cells in treating Ocular Surface Squamous Neoplasia

“…Due to the shortage of stable proliferating OSSN cell lines, lack of pathological tissue samples and challenges in primary cell culture, we employed human oral squamous cancer cell lines Cal27 which were easy to access for this research 46,47 . Although oral squamous cells resemble keratoconjunctival epithelium in physiological structures and biomarkers, they cannot fully represent proliferation characteristics of OSSN tumor cells [48][49][50] . In conclusion, this fundamental study testi ed the underlying reasons behind our clinical investigation, and the proved synergy between IFNα2b and 5-FU substantiated that topical combination chemotherapy would be a promising new therapeutic option for OSSN 18 .…”

Simple oral mucosal epithelial transplantation in a rabbit model

“…Because severe LSCD accompanied by symblepharon is often bilateral, attention has been focused on autologous cell sources other than the corneal limbus, and the oral mucosa has been investigated as an option for ocular surface reconstruction 23 . Direct oral mucosal transplantation without culture after dispase treatment was reported to be successful in rabbits with partial corneal limbal scraping 24 . In that study, grafts were sutured over the wounds at the peripheral corneal-limbal junction.…”

Limbal Niche Cells: a Novel Feeder Cell for Autologous Cultivated Oral Mucosal Epithelial Transplantation

“……”