2021
DOI: 10.1016/j.isci.2021.103531
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Summary Few studies have explored the association between SNPs and alterations in mRNA translation potential. We developed an approach to identify SNPs that can mark allele-specific protein expression levels and could represent sources of inter-individual variation in disease risk. Using MCF7 cells under different treatments, we performed polysomal profiling followed by RNA sequencing of total or polysome-associated mRNA fractions and designed a computational approach to identify SNPs showing a sign… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2

Citation Types

0
2
0

Year Published

2022
2022
2023
2023

Publication Types

Select...
2

Relationship

1
1

Authors

Journals

citations
Cited by 2 publications
(2 citation statements)
references
References 68 publications
0
2
0
Order By: Relevance
“…MicroRNAs might be produced during specific conditions, such as stress or injury that might recognize an RNA transcript and inhibit its translation. Otherwise, single-nucleotide polymorphisms (SNPs) might be responsible for a decrease or arrest of mRNA transcription [26,27]. In order to verify this hypothesis, we sequenced TLR9 gene in a small group of IMNM patients (two with anti-SRP and two with anti-HMGCR auto-antibodies) using the Sanger method.…”
Section: Discussionmentioning
confidence: 99%
“…MicroRNAs might be produced during specific conditions, such as stress or injury that might recognize an RNA transcript and inhibit its translation. Otherwise, single-nucleotide polymorphisms (SNPs) might be responsible for a decrease or arrest of mRNA transcription [26,27]. In order to verify this hypothesis, we sequenced TLR9 gene in a small group of IMNM patients (two with anti-SRP and two with anti-HMGCR auto-antibodies) using the Sanger method.…”
Section: Discussionmentioning
confidence: 99%
“…The recent SNP2TFBS tool ( 7 ), for example, characterizes only around 3 million SNPs and uses only Jaspar database ( 22 ). Of note, provided that variants in UTRs can alter mRNA translation potential ( 41 ) also RBP consensus motifs were included to characterize UTR variants. In addition, our functional characterization in terms of regulatory elements uses our recent CONREL tool ( 18 ), exploiting hence a novel tissue level functional annotation of variants.…”
Section: Discussionmentioning
confidence: 99%