Transmission of Babesia sp to sheep with field-collected Haemaphysalis qinghaiensis

Guan, Guiquan; Yin, Hong; Luo, Jianxun; Lu, Wenshun; Zhang, Q C; Gao, Yulong; Lu, Bingyi

doi:10.1007/s00436-001-0564-5

Cited by 56 publications

(39 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cases were detected in the peak summer season and may be due to more prevalence of ticks during the season (Rashid et al, 2010;Al-Mahmudet al, 2015;Rather et al, 2015). The main vector involved in transmission was Haemaphysalis (Figure 1) as has been reported earlier (Friedhoff, 1997;Guan et al, 2002). The prevalence of the disease in goats has been reported to be lowest (0.75%) among the ruminants (Rather et al, 2015) which may be due to their resistance against diseases.…”

Section: Albumin (G/dl) Globulin (G/dl) A:g Ratio Pre T Post T Pre T mentioning

confidence: 50%

Therapeutic Amelioration of Severely Anaemic Local Kashmiri Goats Affected with Babesiosis

Haq¹,

Tufani²,

Gugjoo³

et al. 2017

Adv. Anim. Vet. Sci.

View full text Add to dashboard Cite

Section: Albumin (G/dl) Globulin (G/dl) A:g Ratio Pre T Post T Pre T mentioning

confidence: 50%

Therapeutic Amelioration of Severely Anaemic Local Kashmiri Goats Affected with Babesiosis

Haq¹,

Tufani²,

Gugjoo³

et al. 2017

Adv. Anim. Vet. Sci.

View full text Add to dashboard Cite

“…Up to the present, the adult Haemaphysalis qinghaiensis tick has been found to transmit four Piroplasma species, which are Theileria uilenbergi, T. luwenshuni, T. sinensis and Babesia motasi (Yin et al 2002a, b, c;Bai et al 2002;Guan et al 2002;Liu et al 2007). Our previous studies indicated that small ruminants are often infected simultaneously by 1 C T. uilenbergi and T. luwenshuni in most endemic regions.…”

Section: Introductionmentioning

confidence: 96%

“…T. sinensis, infective to cattle and yaks, has been reported as being transmitted by H. qinghaiensis (Yin et al 2002a;Bai et al 2002). B. motasi was Wrst isolated by feeding Weld-collected adult H. qinghaiensis on Piroplasmafree sheep under experimental conditions (Guan et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Investigations into the natural infection rate of Haemaphysalis qinghaiensis with Piroplasma using a nested PCR

et al. 2008

View full text Add to dashboard Cite

Here we describe the natural infection rate in China of Haemaphysalis qinghaiensis with four Piroplasma species, namely Theileria uilenbergi, T. luwenshuni, T. sinensis and Babesia motasi. Specifically, a nested PCR was designed based on 18S ribosomal RNA genes and its specificity and sensitivity were established. The result showed that 62 flat adult field H. qinghaiensis ticks (27 females and 35 males) out of 136 (55 females and 81 males) were infected by one or more parasites. All 62 (45.6%) were infected with T. uilenbergi; nine (five males and four females; 6.6%) were infected with T. luwenshuni; two (1.5%) females were infected with T. sinensis; and one (0.7%) male was infected with B. motasi. Twelve (19.4%) were infected with more than one pathogen. There was no significant difference in infection rate between males and females. The high figure 45.6% Theileria infection rate indicates the serious prevalence of theileriosis; while the presence of T. sinensis and B. motasi implies the potential existence of the corresponding diseases in the area studied.

show abstract

“…for small ruminants. It also transmits Theileria sinense, which can infect yaks (Guan et al 2002;Yin et al 2002a, b, c;Yin et al 2004). H. qinghaiensis and the protozoal diseases it transmits are a major economic burden to animal husbandry in China.…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a cDNA clone encoding calreticulin from Haemaphysalis qinghaiensis (Acari: Ixodidae)

et al. 2007

View full text Add to dashboard Cite

The application of anti-tick vaccine has been shown to be the most promising alternative strategy compared to the current use of acaricides that suffer from a number of serious limitations. The success of this method is dependent upon identification and cloning of potential tick vaccine antigens. Previously, we have cloned 21 positive clones (named from Hq02 to Hq22) by immunoscreening complimentary DNA (cDNA) libraries of Haemaphysalis qinghaiensis; however, some of those clones did not contain open reading frames (ORF). In this study, we amplified the entire sequence of Hq07 by using rapid amplification of the cDNA ends. Hq07 contains an ORF of 1,233 bp that encodes for 410 amino acid residues with a coding capacity of 47 kDa. Search of the cloned sequences against GenBank revealed that Hq07 is a calreticulin (CRT)-similar clone and designated HqCRT. Expression analysis by reverse transcription-polymerase chain reaction showed that this gene is ubiquitously expressed at different developmental stages and in different tissues of H. qinghaiensis. The gene was expressed as glutathione S-transferase-fused proteins in a prokaryotic system. Western blot analysis revealed that native HqCRT was secreted into their hosts by ticks during blood sucking. Vaccination of sheep with rHqCRT conferred protective immunity against ticks, resulting in 54.3% mortality in adult ticks, compared to the 38.7% death rate in the control group. These results demonstrated that rHqCRT might be a useful vaccine candidate antigen for biological control of H. qinghaiensis.

show abstract

Transmission of Babesia sp to sheep with field-collected Haemaphysalis qinghaiensis

Cited by 56 publications

References 7 publications

Therapeutic Amelioration of Severely Anaemic Local Kashmiri Goats Affected with Babesiosis

Therapeutic Amelioration of Severely Anaemic Local Kashmiri Goats Affected with Babesiosis

Investigations into the natural infection rate of Haemaphysalis qinghaiensis with Piroplasma using a nested PCR

Cloning and characterization of a cDNA clone encoding calreticulin from Haemaphysalis qinghaiensis (Acari: Ixodidae)

Contact Info

Product

Resources

About