Transmission Genetics of Microbotryum violaceum (Ustilago violacea): A Case History

Garber, E. D.; Ruddat, Manfred

doi:10.1016/s0065-2164(02)51003-0

Cited by 31 publications

(22 citation statements)

References 46 publications

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“…The phenomenon was first observed because of the inability to isolate sporidia of one of the two mating types from certain strains of the pathogen (38,63,87,100). This mating-type bias was investigated for a number of possible causes, including spore killers and meiotic drive (83).…”

Section: Mating-type Bias and Haplo-lethal Allelesmentioning

confidence: 99%

Mating System of the Anther Smut Fungus Microbotryum violaceum : Selfing under Heterothallism

et al. 2008

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Section: Mating-type Bias and Haplo-lethal Allelesmentioning

confidence: 99%

Mating System of the Anther Smut Fungus Microbotryum violaceum : Selfing under Heterothallism

et al. 2008

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“…It is comreplicated with a separate culture of the same fungal strain. monly studied as a model for disease ecology and fungal genet-DNA was isolated as before from chromosome bands correics Garber and Ruddat 2002, and sponding to plugs 2 and 3 in Figure 1A and were similarly references therein). Collections of the fungus from different used for PCR amplification and sequencing of 30 clones per host plants probably represent cryptic species, but the taxonchromosome.…”

Section: Violaceummentioning

confidence: 99%

Repeat-Induced Point Mutation and the Population Structure of Transposable Elements in Microbotryum violaceum

2005

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Repeat-induced point mutation (RIP) is a genome defense in fungi that hypermutates repetitive DNA and is suggested to limit the accumulation of transposable elements. The genome of Microbotryum violaceum has a high density of transposable elements compared to other fungi, but there is also evidence of RIP activity. This is the first report of RIP in a basidiomycete and was obtained by sequencing multiple copies of the integrase gene of a copia-type transposable element and the helicase gene of a Helitron-type element. In M. violaceum, the targets for RIP mutations are the cytosine residues of TCG trinucleotide combinations. Although RIP is a linkage-dependent process that tends to increase the variation among repetitive sequences, a chromosome-specific substructuring was observed in the transposable element population. The observed chromosome-specific patterns are not consistent with RIP, but rather suggest an effect of gene conversion, which is also a linkage-dependent process but results in a homogenization of repeated sequences. Particular sequences were found more widely distributed within the genome than expected by chance and may reflect the recently active variants. Therefore, sequence variation of transposable elements in M. violaceum appears to be driven by selection for transposition ability in combination with the contextspecific forces of the RIP and gene conversion.

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“…Cryptococcus neoformans; Goodwin & Poulter, 2001), and how completely an organism must restrict outcrossing to decrease repetitive element diversity is unknown. Here, I describe repetitive DNA in the genome of Microbotryum violaceum, a highly selfing basidiomycete fungus (Antonovics, Hood & Partain, 2002;Garber & Ruddat, 2002;. The study is based on a genome sampling approach that could also be used to investigate the nuclear content in other organisms where complete genome sequencing is not feasible.…”

Section: Introductionmentioning

confidence: 99%

Repetitive DNA in the automictic fungus Microbotryum violaceum

Hood

2005

Genetica

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The small genomes of fungi are expected to have little repetitive content other than rDNA genes. Moreover, among asexual or highly selfing lineages, the diversity of repetitive elements is also expected to be very low. However, in the automictic fungus Microbotryum violaceum, a very large proportion of random DNA fragments from the autosomes and the fungal sex chromosomes are repetitive in nature, either as retrotransposon or helicase sequences. Among the retrotransposon sequences, examples were found from each major kind of elements, including copia, gypsy, and non-LTR sequences. The most numerous were copialike elements, which are believed to be rare in fungi, particularly among basidiomycetes. The many helicase sequences appear to belong to the recently discovered Helitron type of transposable elements. Also, sequences that could not be identified as a known type of gene were also very repetitive within the database of random fragments from M. violaceum. The differentiated pair of fungal sex chromosomes and suppression of recombination may be the major forces determining the highly repetitive content in the small genome of M. violaceum.

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Transmission Genetics of Microbotryum violaceum (Ustilago violacea): A Case History

Cited by 31 publications

References 46 publications

Mating System of the Anther Smut Fungus Microbotryum violaceum : Selfing under Heterothallism

Mating System of the Anther Smut Fungus Microbotryum violaceum : Selfing under Heterothallism

Repeat-Induced Point Mutation and the Population Structure of Transposable Elements in Microbotryum violaceum

Repetitive DNA in the automictic fungus Microbotryum violaceum

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