2011
DOI: 10.4142/jvs.2011.12.3.267
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Abstract: The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three ejaculates per bull were evaluated. Frozen semen samples were thawed and the acrosomal region of sperm cells was evaluated by transmission electron microscopy (TEM) before (n = 18) and after (n = 18) Percoll centrifugation. The evaluation of 20 sperm heads from each of the 36 samples analy… Show more

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Cited by 22 publications
(19 citation statements)
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“…No differences in between the fertilization rates were seen between the Percoll gradient and our EC separation system. The previous findings regarding the effects of Percoll, PVP and centrifugation [5,18] give even more value to our results, since with our new device we can replace the Percoll gradients entirely without reducing the embryo development rate. Our results are more important if we support them with the Simon's data [31], which shows that using the electric charge was possible to select the most low percentage of sperm DNA damage.…”
Section: Discussionsupporting
confidence: 58%
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“…No differences in between the fertilization rates were seen between the Percoll gradient and our EC separation system. The previous findings regarding the effects of Percoll, PVP and centrifugation [5,18] give even more value to our results, since with our new device we can replace the Percoll gradients entirely without reducing the embryo development rate. Our results are more important if we support them with the Simon's data [31], which shows that using the electric charge was possible to select the most low percentage of sperm DNA damage.…”
Section: Discussionsupporting
confidence: 58%
“…Several techniques have been developed to recover a homogeneous population of highly motile sperm cells. These techniques are used for removing seminal plasma, dead cells, abnormal sperm, cryoprotective agents, and other factors [5]. The techniques include Percoll density gradient centrifugation, swim-up migration, washing by centrifugation, glass wool filtration and several others [6][7][8][9][10][11][12].…”
Section: Introductionmentioning
confidence: 99%
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“…Such damage, along with vacuolization of the mitochondria, might be associated with osmotic shock [10] suffered during freezing-thawing. Ultrastructural changes in the acrosomes and mitochondria were described as similar to those seen in bovine and ovine sperm frozen by conventional techniques [59,60]. González-Fernández et al [10] also suggest that all these kinds of ultrastructural alteration in equine sperm are likely produced by osmotic changes.…”
Section: Discussionmentioning
confidence: 85%
“…Furthermore, it is necessary to separate motile sperm from the other components (seminal plasma, extender, and/or undesired cells) that are contained in the freezing straw for optimal fertilization rates during IVF (Parrish J 1991;Parrish J. J. et al 1995). Several techniques have been developed to recover a homogeneous population of highly motile sperm cells (Oliveira et al 2011). However, not all separation methods are ideal, some are not fast, easy, or sufficiently economical for routine use in an IVF lab (Henkel and Schill 2003).…”
Section: Introductionmentioning
confidence: 99%