Translocation breakpoints inFHIT and FRA3B in both homologs of chromosome 3 in an esophageal adenocarcinoma

Reciprocal translocations in breast tumor cell lines: Cloning of a t(3;20) that targets the FHIT gene

“…A t(3;12)(p14.2;q12) was found in a pleomorphic adenoma of the parotid gland; it fuses FHIT to the HMGA2 gene on chromosome 12 (Geurts et al, 1997). Recently, two translocations, t(3;4)(p14.2;p16) and t(3;16)(p14.2;p13), were characterized in esophageal adenocarcinomas, both accompanied by different large deletions around FHIT exon 5 (Fang et al, 2001); further resembling what we found for the t(3.20), the t(3;16)(p14.2;p13) showed the production of a chimeric transcript generated by the fusion of FHIT to a nonfunctional transcribed and spliced sequence on chromosome 16. The t(3;20) breakpoint lies close to the breakpoint of t(3;4) and t(3;16) translocations of esophageal carcinoma (Fang et al, 2001) and telomeric to the breakpoint of t(3;8) associated with hereditary RCC (Gemmill et al, 1998).…”

“…4B). For the t(3;20)(p14;p11) of BrCa‐MZ‐02, DNA of 850A6, 947B1, 750F1, and 805F12 YAC clones specific for the FHIT / FRA3B region in 3p14 were used as probes (Wang et al, 2000; Fang et al, 2001). The 850A6 YAC clone extends from a region proximal (centromeric) to the FHIT locus to the proximal (5′) end of FHIT intron 5.…”

Reciprocal translocations in breast tumor cell lines: Cloning of a t(3;20) that targets the FHIT gene

“…A t(3;12)(p14.2;q12) was found in a pleomorphic adenoma of the parotid gland; it fuses FHIT to the HMGA2 gene on chromosome 12 (Geurts et al, 1997). Recently, two translocations, t(3;4)(p14.2;p16) and t(3;16)(p14.2;p13), were characterized in esophageal adenocarcinomas, both accompanied by different large deletions around FHIT exon 5 (Fang et al, 2001); further resembling what we found for the t(3.20), the t(3;16)(p14.2;p13) showed the production of a chimeric transcript generated by the fusion of FHIT to a nonfunctional transcribed and spliced sequence on chromosome 16. The t(3;20) breakpoint lies close to the breakpoint of t(3;4) and t(3;16) translocations of esophageal carcinoma (Fang et al, 2001) and telomeric to the breakpoint of t(3;8) associated with hereditary RCC (Gemmill et al, 1998).…”

“…4B). For the t(3;20)(p14;p11) of BrCa‐MZ‐02, DNA of 850A6, 947B1, 750F1, and 805F12 YAC clones specific for the FHIT / FRA3B region in 3p14 were used as probes (Wang et al, 2000; Fang et al, 2001). The 850A6 YAC clone extends from a region proximal (centromeric) to the FHIT locus to the proximal (5′) end of FHIT intron 5.…”

Effects of FHIT gene on proliferation and apoptosis of osteosarcoma cells

“…Therefore, FHIT gene is involved in the regulation of cell apoptosis and cell cycle process. The inhibitory effect of FHIT gene on tumor growth may be achieved by promoting apoptosis of tumor cells and inhibiting the growth of tumor cells (19,20). In this study, it was found that FHIT gene was abnormally expressed in Saos2 osteosarcoma cell line, and the FHIT gene expression was suppressed in Saos2 osteosarcoma cell line compared with that in normal osteoblast cell line, including transcription and translation.…”

Analysis of the t(3;8) of hereditary renal cell carcinoma: a palindrome-mediated translocation

“…PATRR3 is located in or in proximity to FRA3B, in which frequent deletions and translocations are seen in various tumor cells (29, 30, 51). Therefore we investigated the possibility of a relationship between PATRR3 instability and FRA3B deletions in normal human samples and in APH-treated human-mouse somatic cell hybrids.…”

Reciprocal translocations in breast tumor cell lines: Cloning of a t(3;20) that targets the FHIT gene

“…A t(3;12)(p14.2;q12) was found in a pleomorphic adenoma of the parotid gland; it fuses FHIT to the HMGA2 gene on chromosome 12 (Geurts et al, 1997). Recently, two translocations, t(3;4)(p14.2;p16) and t(3;16)(p14.2;p13), were characterized in esophageal adenocarcinomas, both accompanied by different large deletions around FHIT exon 5 (Fang et al, 2001); further resembling what we found for the t(3.20), the t(3;16)(p14.2;p13) showed the production of a chimeric transcript generated by the fusion of FHIT to a nonfunctional transcribed and spliced sequence on chromosome 16. The t(3;20) breakpoint lies close to the breakpoint of t(3;4) and t(3;16) translocations of esophageal carcinoma (Fang et al, 2001) and telomeric to the breakpoint of t(3;8) associated with hereditary RCC (Gemmill et al, 1998).…”

“…4B). For the t(3;20)(p14;p11) of BrCa‐MZ‐02, DNA of 850A6, 947B1, 750F1, and 805F12 YAC clones specific for the FHIT / FRA3B region in 3p14 were used as probes (Wang et al, 2000; Fang et al, 2001). The 850A6 YAC clone extends from a region proximal (centromeric) to the FHIT locus to the proximal (5′) end of FHIT intron 5.…”

Effects of FHIT gene on proliferation and apoptosis of osteosarcoma cells

“…Therefore, FHIT gene is involved in the regulation of cell apoptosis and cell cycle process. The inhibitory effect of FHIT gene on tumor growth may be achieved by promoting apoptosis of tumor cells and inhibiting the growth of tumor cells (19,20). In this study, it was found that FHIT gene was abnormally expressed in Saos2 osteosarcoma cell line, and the FHIT gene expression was suppressed in Saos2 osteosarcoma cell line compared with that in normal osteoblast cell line, including transcription and translation.…”

Analysis of the t(3;8) of hereditary renal cell carcinoma: a palindrome-mediated translocation

“…PATRR3 is located in or in proximity to FRA3B, in which frequent deletions and translocations are seen in various tumor cells (29, 30, 51). Therefore we investigated the possibility of a relationship between PATRR3 instability and FRA3B deletions in normal human samples and in APH-treated human-mouse somatic cell hybrids.…”

Reciprocal translocations in breast tumor cell lines: Cloning of a t(3;20) that targets the FHIT gene

“…A t(3;12)(p14.2;q12) was found in a pleomorphic adenoma of the parotid gland; it fuses FHIT to the HMGA2 gene on chromosome 12 (Geurts et al, 1997). Recently, two translocations, t(3;4)(p14.2;p16) and t(3;16)(p14.2;p13), were characterized in esophageal adenocarcinomas, both accompanied by different large deletions around FHIT exon 5 (Fang et al, 2001); further resembling what we found for the t(3.20), the t(3;16)(p14.2;p13) showed the production of a chimeric transcript generated by the fusion of FHIT to a nonfunctional transcribed and spliced sequence on chromosome 16. The t(3;20) breakpoint lies close to the breakpoint of t(3;4) and t(3;16) translocations of esophageal carcinoma (Fang et al, 2001) and telomeric to the breakpoint of t(3;8) associated with hereditary RCC (Gemmill et al, 1998).…”

“…4B). For the t(3;20)(p14;p11) of BrCa‐MZ‐02, DNA of 850A6, 947B1, 750F1, and 805F12 YAC clones specific for the FHIT / FRA3B region in 3p14 were used as probes (Wang et al, 2000; Fang et al, 2001). The 850A6 YAC clone extends from a region proximal (centromeric) to the FHIT locus to the proximal (5′) end of FHIT intron 5.…”

Effects of FHIT gene on proliferation and apoptosis of osteosarcoma cells

“…Therefore, FHIT gene is involved in the regulation of cell apoptosis and cell cycle process. The inhibitory effect of FHIT gene on tumor growth may be achieved by promoting apoptosis of tumor cells and inhibiting the growth of tumor cells (19,20). In this study, it was found that FHIT gene was abnormally expressed in Saos2 osteosarcoma cell line, and the FHIT gene expression was suppressed in Saos2 osteosarcoma cell line compared with that in normal osteoblast cell line, including transcription and translation.…”

Analysis of the t(3;8) of hereditary renal cell carcinoma: a palindrome-mediated translocation

“…PATRR3 is located in or in proximity to FRA3B, in which frequent deletions and translocations are seen in various tumor cells (29, 30, 51). Therefore we investigated the possibility of a relationship between PATRR3 instability and FRA3B deletions in normal human samples and in APH-treated human-mouse somatic cell hybrids.…”