Translation of ceragenin affinity for bacteria to an imaging reagent for infection

Bandara, Nilantha; Li, Yubo; Diebolder, Philipp; Mpoy, Cedric; Gu, Xiaobo; Khanal, Pitambar; Deng, Shenglou; Rogers, Buck E.; Savage, Paul B.

doi:10.1039/c9ra02226k

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“…As demonstrated, CSA-13 accumulates in the bladder in the first 4 h, whereas after 8 h it is also detected in the liver. After 24 h, the fluorescence intensity appeared to be derived from labeled ceragenin decay, which may indicate the complete elimination of this compound within the 24 h. This would be in agreement with previous studies suggesting that ceragenin accumulates in the kidney, among other areas, which further supports our validity in the use of ceragenin in UTI 44 . These results were confirmed by analysis of animal excreta (Figs.…”

Section: Discussionsupporting

confidence: 92%

Ceragenin CSA-13 displays high antibacterial efficiency in a mouse model of urinary tract infection

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et al. 2022

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Ceragenins (CSAs) are synthetic, lipid-based molecules that display activities of natural antimicrobial peptides. Previous studies demonstrated their high in vitro activity against pathogens causing urinary tract infections (UTIs), but their efficiency in vivo was not explored to date. In this study, we aimed to investigate the bactericidal efficiency of ceragenins against E. coli (Xen14 and clinical UPEC strains) isolates both in vitro and in vivo, as well to explore CSA-13 biodistribution and ability to modulate nanomechanical alterations of infected tissues using animal model of UTI. CSA-44, CSA-131 and particularly CSA-13 displayed potent bactericidal effect against tested E. coli strains, and this effect was mediated by induction of oxidative stress. Biodistribution studies indicated that CSA-13 accumulates in kidneys and liver and is eliminated with urine and bile acid. We also observed that ceragenin CSA-13 reverses infection-induced alterations in mechanical properties of mouse bladders tissue, which confirms the preventive role of CSA-13 against bacteria-induced tissue damage and potentially promote the restoration of microenvironment with biophysical features unfavorable for bacterial growth and spreading. These data justify the further work on employment of CSA-13 in the treatment of urinary tract infections.

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Section: Discussionsupporting

confidence: 92%

Ceragenin CSA-13 displays high antibacterial efficiency in a mouse model of urinary tract infection

Wnorowska

Piktel

Deptuła

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

Translation of ceragenin affinity for bacteria to an imaging reagent for infection

Abstract: Infection imaged via autoradiography with ceragenin conjugated to a copper radiolabel.

Cited by 1 publication

References 18 publications

Ceragenin CSA-13 displays high antibacterial efficiency in a mouse model of urinary tract infection

Ceragenin CSA-13 displays high antibacterial efficiency in a mouse model of urinary tract infection

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