1992
DOI: 10.1089/ard.1992.2.111
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: A detailed comparison was made of the concentration dependence of translation inhibition by phosphorothioate and phosphodiester oligodeoxynucleotides of the same anti-beta-globin sequence in cell-free systems using beta-globin mRNA and unrelated mRNAs as controls. The results confirm that at low concentrations the phosphorothioate oligomer is more potent as an antisense compound, while at higher concentration (greater than 4 microM) it exhibits more nonspecific inhibition than the phosphodiester oligomer for R… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
10
0

Year Published

1993
1993
2005
2005

Publication Types

Select...
7
2

Relationship

1
8

Authors

Journals

citations
Cited by 34 publications
(10 citation statements)
references
References 21 publications
0
10
0
Order By: Relevance
“…This ratio is lower than what has been reported to be necessary to achieve inhibition by phosphorothioate oligonucleotides (e.g. 10:1 for oligonucleotides Ͼ20 bases in length and 50:1 for oligonucleotides 10 -15 bases) (26). The effects of phosphodiester and phosphorothioate oligonucleotides at all concentrations tested were largely nonspecific (i.e.…”
Section: Discussionmentioning
confidence: 60%
“…This ratio is lower than what has been reported to be necessary to achieve inhibition by phosphorothioate oligonucleotides (e.g. 10:1 for oligonucleotides Ͼ20 bases in length and 50:1 for oligonucleotides 10 -15 bases) (26). The effects of phosphodiester and phosphorothioate oligonucleotides at all concentrations tested were largely nonspecific (i.e.…”
Section: Discussionmentioning
confidence: 60%
“…In most cases the same target, but different oligos such as mismatch and random sequences, have been used as controls. We chose to carry out the translation studies this way because we felt that in terms of the actual therapeutic situation this is a more realistic control (37). A significant translation inhibition of the BMV mRNA particularly with the PS2-derivative suggests an increased non-specific hybridization compared to the P02or POS-derivatives.…”
Section: Discussionmentioning
confidence: 99%
“…The present investigation has been directed to determine such properties using a 17-mer all-PS2 sequence complementary to the coding region of the rabbit ,B-globin mRNA. Earlier results showing translation arrest of this mRNA in cell-free systems by both normal (PO2) and phosphorotioate (POS) oligonucleotides (7,9,37,38) have led us to use the same sequence in the present investigation.…”
Section: Introductionmentioning
confidence: 99%
“…In a slower reaction, the nucleophilic 5′-hydroxyl group then displaces tetrazole to form the phosphite triester. 57,58 The phosphite intermediate then undergoes sulfurization to give phosphorothioate triesters.…”
Section: Discussionmentioning
confidence: 99%