Transgenic plant production mediated by Agrobacterium in Indica rice

Hamid, Rizwan; Toriyama, Kinya; Hinata, Kokichi; Yokoi, Shuji

doi:10.1007/s002990050108

Cited by 78 publications

(104 citation statements)

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“…Successful Agrobacterium-mediated transformation has been reported in rice (Aldemita and Hodges, 1996;Hiei et al, 1997;Hiei et al, 1994;Rashid et al, 1996;Toki et al, 1997), maize (Ishida et al, 1996), barley (Tingay et al, 1997), wheat Cheng et al, 1997) and, recently, sorghum (Zhao et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency

et al. 2004

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The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection.

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Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency

et al. 2004

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“…In particular, genotype and the composition of culture medium are important factors for successful embryogenic callus induction and regeneration of plant. Significant genotypic variations in Japonica and Indica rice have been reported (Abe and Futsuhara 1986;Rashid et al 1996;Khanna and Raina 1998).…”

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confidence: 99%

“…High efficiency tissue culture system for Japonica rice has already been established by the numerous researchers including the pioneer work of Hiei et al 1994. In Indica rice, several applicable tissue culture methods for subculture and regeneration have been reported (Peng and Hodges 1989;Aldemita and Hodges 1996;Rashid et al 1996;Khanna and Raina 1998). However, only few tissue culture methods have been reported for Javanica rice.…”

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confidence: 99%

Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars

Rachmawati

Anzai

2006

Plant Biotechnology

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The purpose of this study was to develop a reproducible efficient procedure for the transformation of Javanica rice cultivars from Indonesia. Five rice cultivars cultivated in Indonesia now were examined for their capacity on the callus growth, plant regeneration and transformation. Their potential was affected by genotype and medium. Regarding the quality of callus, type I calli produced higher plant regeneration frequency than type II calli. So type I calli were inoculated with A. tumefaciens harboring binary plasmid pAFT14, which had a hygromycin resistance (hpt) gene and a gus gene. In this study, we examined two media for two steps in the tissue culture process for transformation, i.e. callus inducing and plant regeneration. The results show that C-modified medium was the suitable media for callus induction in the most Javanica rice cultivars. Agrobacterium-mediated transformation system has been extended to five Javanica rice cultivars. Among them, Rojolele consistently gave the best performance.Key words: Callus, Javanica rice, transformation.Plant Biotechnology 23, 521-524 (2006) This article can be found at http://www.jspcmb.jp/ Tissue Culture Note tolerant to drought (gogo rice). The callus induction rate was calculated after 4 weeks of culture as the percentage of the seeds producing callus.As results, significant difference was found in callus induction rate and quality of callus induced among the five genotypes of Javanica rice (Table 2). Although a considerable variation was observed, all genotypes of rice can induce callus on both media. Two types of calli, embryogenic and non-embryogenic ones, were obtained from the embryo scutellum. The embryogenic calli (type-I) were relatively dry, milky white in color, compact and nodular in appearance ( Figure 1A, B). In contrast, non-embryogenic calli (type-II) were soft and watery, mostly white but some brown in color ( Figure 1C, D). Four weeks after induction, the calli were subcultured onto fresh medium, which had the same composition as that used for callus induction. Following the subculture, the type-I calli grew about 5 to 10-fold in volume 30 days after transfer. However, the type-II calli turned brown and died.In general, callus induction rate of the five Javanica rice cultivars on C-modified medium was higher than that on MS2 medium (Table 2). This finding is consistent with the results reported by Ogawa (2000) that Cmedium is applicable to a wider range of cultivars including Indica, Japonica and Javanica subspecies. The induction rate of type-I calli from all the five Javanica rice cultivars on C-modified medium was higher than that on MS2 medium, especially for Rojolele. On Cmodified medium, the induction rate of Rojolele was 10 times higher than one on MS2. On the other hand, the induction rate of type-II calli of the five cultivars on MS2 medium was almost the same as that on C-modified medium (Table 2). To determine the regeneration ability, the initiated calli were transferred to a regeneration medium which was composed of ma...

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“…Until now, routine and efficient Agrobacterium-mediated transformation systems have been developed for japonica, javanica and indica cultivars of Asian rice (Oryza sativa L.) and for the new rice for Africa (NERICA, O. sativa 9 O. glaberrima) (Dong et al 1996;Hiei and Komari 2006;Hiei et al 1994;Ishizaki and Kumashiro 2008;Rashid et al 1996;Toki 1997;Toki et al 2006). In Nipponbare, a model variety of japonica rice, the generation of transgenic plantlets at high frequency was successfully achieved within a month of inoculation of mature seeds .…”

Section: Introductionmentioning

confidence: 99%

Visual selection allows immediate identification of transgenic rice calli efficiently accumulating transgene products

Saika

Toki

2009

Plant Cell Rep

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In genetic transformation systems, antibiotic resistance genes are routinely used as powerful markers for selecting transformed cells from surrounding non-transformed cells. However, simultaneous use of the gene encoding green fluorescent protein (GFP) and an antibiotic resistance gene facilitates the selection process, since it allows visible selection of transformed cells. Here, we report the development of a visual selection system for transformed cells using a GFP marker without selection against antibiotics after Agrobacterium-mediated transformation in rice. Both GFP protein levels and GFP fluorescence in calli isolated by visual selection were higher than in calli selected on hygromycin (Hyg), suggesting that transgenic calli hyper-accumulating GFP were efficiently obtained by selection using GFP fluorescence itself rather than Hyg resistance. Furthermore, gfp transcripts in calli isolated by visual selection were more abundant than under Hyg selection; in contrast, transcript levels of hpt in calli selected visually were comparable to those obtained under Hyg selection. These results suggest that there was no correlation between hpt and gfp expression levels, despite the fact that they are aligned in tandem on an integrated locus after selection by either GFP fluorescence or Hyg resistance. This fact indicates that positional effects can influence the expression of each transgene differently, even when they are located in tandem at the same locus. In summary, based on our results, we discuss a model system for rice cell culture transformation for the production of recombinant proteins using visual selection.

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Transgenic plant production mediated by Agrobacterium in Indica rice

Cited by 78 publications

References 0 publications

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency

Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars

Visual selection allows immediate identification of transgenic rice calli efficiently accumulating transgene products

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