Transgenic grain legumes obtained byin planta electroporation-mediated gene transfer

Abstract

Electroporation-mediated gene transfer into intact plant tissues was demonstrated in pea, cowpea, lentil, and soybean plants. Transient expression of a chimeric gus reporter gene was used to monitor the uptake and expression of the introduced DNA in electroporated nodal axillary buds in vivo. The branches that grew out of the nodal meristems were chimeric and expressed the introduced gene up to 20 d after electroporation. Transgenic R1 pea, lentil, and cowpea plants were recovered from seeds originating on these chimeric branches as shown by Southern blot hybridization and GUS expression. Transgenic R2 soybean and lentil plants were also obtained. Segregation ratios in these populations showed a strong bias against transgene presence or expression.

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

“…For instance, this method reduced the generation time by three months in pea (Hassan et al, 2009). Alternatively, in-planta transformation that may circumvent the need of tissue culture regeneration steps has been reported for M. truncatula (Trieu et al, 2000), peanuts (Rohini and Rao, 2000), pea (Chowrira et al, 1998), pigeonpea (Ramu et al, 2012), cowpea (Adesoye et al, 2008), as well as lentil and soybean (Chowrira et al, 1996).…”

Induction of dental pulp stem cell differentiation into odontoblasts by electroporation-mediated gene delivery of growth/differentiation factor 11 (Gdf11)

“…A potential method to overcome this dilemma is electroporation using pulsed electric fields to deliver DNA. [14][15][16][17] Many tissues respond to electroporation, and handling is relatively easy and rapid. 18 Electroporation also has been used to deliver genes to living animals.…”

Nanoparticles-Based Delivery Systems in Plant Genetic Transformation

“…On applying electric current (pulses), the transient increase in porosity of the cell membrane allows DNA to easily transfer and enter into the cytoplasm. After sometime, the pores retain their porosity; as a result, DNA is unable to escape from the cell (Neumann et al 1982;Chowrira et al 1996;To et al 1996;Lurquin 1997). Although electroporation seems to be an easy and effective method, its applications are limited to only a few species.…”