Transgenic expression of TaTLP1, a thaumatin-like protein gene, reduces susceptibility to common root rot and leaf rust in wheat

Cui, Zhongchi; Liang, Fang Ting; Zhang, Jiarui; Wang, Fei; Liu, Daqun; Wang, Haiyan

doi:10.1016/j.cj.2021.03.021

Cited by 16 publications

(20 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of TLP genes in fungal resistance has been reported in earlier studies against numerous pathogens including Bipolaris sorokiniana ( Cui et al, 2021 ), Fusarium sp. ( Mackintosh et al, 2007 ), Microdochium nivale ( Kuwabara et al, 2002 ), Puccinia.…”

Section: Discussionmentioning

confidence: 79%

“…( Mackintosh et al, 2007 ), Microdochium nivale ( Kuwabara et al, 2002 ), Puccinia. Triticina ( Cui et al, 2021 ), Rhizoctonia solani ( Naseri et al, 2012 ), and Verticillium dahlia ( Li Z. et al, 2020 ) in wheat and other plant species. Bioassay of Plasmopara viticola in transgenic V. vinifera showed that the overexpression of VaTLP gene helped in the reduction of hyphe growth ( He et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…Each TLP consists of a conserved REDDD motif and a thaumatin signature motif G-X-[GF]-X-C-X-T- [GA]-D-C-X- (1,2)-G-X-(2,3)-C. The REDDD motif is involved in receptor binding for its antifungal action ( Liu et al, 2010a ). Numerous studies reported that the overexpression of TLPs provides significant resistance against various fungi in both dicot and monocot plants ( Datta et al, 1999 ; Rajam et al, 2007 ; Wang et al, 2010 ; Cui et al, 2021 ). The transgenic Arabidopsis thaliana with an overexpressing PR5 gene ( ObTLP1 ) of Ocimum basilicum showed enhanced tolerance against Sclerotinia sclerotiorum and Botrytis cinerea ( Misra et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…The transgenic Arabidopsis thaliana with an overexpressing PR5 gene ( ObTLP1 ) of Ocimum basilicum showed enhanced tolerance against Sclerotinia sclerotiorum and Botrytis cinerea ( Misra et al, 2016 ). The improved resistance of transgenic wheat against common root rot and leaf rust has been observed in TaTLP1 -overexpressing transgenic lines ( Cui et al, 2021 ). The mechanism of action of TLPs in fungal resistance is ambiguous; however, it has been presumed that they work by degradation and permeabilization of the fungal cell walls ( Abad et al, 1996 ; Liu et al, 2010a ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response

Sharma

Rajput

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Thaumatin-like proteins (TLPs) are related to pathogenesis-related-5 (PR-5) family and involved in stress response. Herein, a total of 93 TLP genes were identified in the genome of Triticum aestivum. Further, we identified 26, 27, 39, and 37 TLP genes in the Brachypodium distachyon, Oryza sativa, Sorghum bicolor, and Zea mays genomes for comparative characterization, respectively. They could be grouped into small and long TLPs with conserved thaumatin signature motif. Tightly clustered genes exhibited conserved gene and protein structure. The physicochemical analyses suggested significant differences between small and long TLPs. Evolutionary analyses suggested the role of duplication events and purifying selection in the expansion of the TLP gene family. Expression analyses revealed the possible roles of TLPs in plant development and abiotic and fungal stress response. Recombinant expression of TaTLP2-B in Saccharomyces cerevisiae provided significant tolerance against cold, heat, osmotic, and salt stresses. The results depicted the importance of TLPs in cereal crops that would be highly useful in future crop improvement programs.

show abstract

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response

Sharma

Rajput

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…OsIP-4 is a thaumatin-like protein (TLP), which plays an important role in combating plant pathogen infection. The TaTLP1-OE lines’ resistance to leaf rust and common root rot was improved [ 30 ]. In the future, we will focus on the study of the interaction between MoPtep1 and its candidate targets in host rice cells and then elucidate the mechanism that marks peroxisomes as the target organelle by effector proteins to hijack the plant immunity response.…”

Section: Discussionmentioning

confidence: 99%

Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae

Ning

Xie

et al. 2022

IJMS

View full text Add to dashboard Cite

Rice blast caused by Magnaporthe oryzae is one of the most serious fungous diseases in rice. In the past decades, studies have reported that numerous M. oryzae effectors were secreted into plant cells to facilitate inoculation. Effectors target host proteins to assist the virulence of pathogens via the localization of specific organelles, such as the nucleus, endoplasmic reticulum, chloroplast, etc. However, studies on the pathogenesis of peroxisome-targeting effectors are still limited. In our previous study, we analyzed the subcellular localization of candidate effectors from M. oryzae using the agrobacterium-mediated transient expression system in tobacco and found that MoPtep1 (peroxisomes-targeted effector protein 1) localized in plant peroxisomes. Here, we proved that MoPtep1 was induced in the early stage of the M. oryzae infection and positively regulated the pathogenicity, while it did not affect the vegetative growth of mycelia. Subcellular localization results showed that MoPtep1 was localized in the plant peroxisomes with a signal peptide and a cupredoxin domain. Sequence analysis indicated that the homologous protein of MoPtep1 in plant-pathogenic fungi was evolutionarily conserved. Furthermore, MoPtep1 could suppress INF1-induced cell death in tobacco, and the targeting host proteins were identified using the Y2H system. Our results suggested that MoPtep1 is an important pathogenic effector in rice blast.

show abstract

Genome-wide identification of thaumatin-like protein family in pear and functional analysis their roles in pollen growth

Zhang,

Liu,

Tang

et al. 2024

Plant Growth Regul

View full text Add to dashboard Cite

Transgenic expression of TaTLP1, a thaumatin-like protein gene, reduces susceptibility to common root rot and leaf rust in wheat

Cited by 16 publications

References 21 publications

Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response

Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response

Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae

Genome-wide identification of thaumatin-like protein family in pear and functional analysis their roles in pollen growth

Contact Info

Product

Resources

About