Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality

Tamás, Cecília; Kisgyörgy, Boglárka N.; Rakszegi, Marianna; Wilkinson, Mark; Yang, Moon Sik; Láng, László; Tamás, László; Bedö, Z.

doi:10.1007/s00299-009-0716-0

Cited by 63 publications

(23 citation statements)

References 46 publications

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“…There have been several attempts through mutant selection and engineering genes encoding key amino acid biosynthesis pathway enzymes to increase free essential amino acids in crop plants (2), but with limited success (3). A promising strategy is the genetic engineering of genes encoding proteins with high nutritional value into food crops (1,(4)(5)(6). However, despite promises that genetically modified (GM) crops could make a significant contribution to achieving global food security, the new-generation GM varieties are primarily used for industrial crops, such as cotton and animal fodder (7), and few have been commercialized.…”

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confidence: 99%

Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber

Chakraborty

Agrawal

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Protein deficiency is the most crucial factor that affects physical growth and development and that increases morbidity and mortality especially in developing countries. Efforts have been made to improve protein quality and quantity in crop plants but with limited success. Here, we report the development of transgenic potatoes with enhanced nutritive value by tuber-specific expression of a seed protein, AmA1 (Amaranth Albumin 1), in seven genotypic backgrounds suitable for cultivation in different agro-climatic regions. Analyses of the transgenic tubers revealed up to 60% increase in total protein content. In addition, the concentrations of several essential amino acids were increased significantly in transgenic tubers, which are otherwise limited in potato. Moreover, the transgenics also exhibited enhanced photosynthetic activity with a concomitant increase in total biomass. These results are striking because this genetic manipulation also resulted in a moderate increase in tuber yield. The comparative protein profiling suggests that the proteome rebalancing might cause increased protein content in transgenic tubers. Furthermore, the data on field performance and safety evaluation indicate that the transgenic potatoes are suitable for commercial cultivation. In vitro and in vivo studies on experimental animals demonstrate that the transgenic tubers are also safe for human consumption. Altogether, these results emphasize that the expression of AmA1 is a potential strategy for the nutritional improvement of food crops.allergenecity | essential amino acids | nutritional health

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confidence: 99%

Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber

Chakraborty

Agrawal

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…"Cadenza") and transformation method was applied. Transformation efficiency was slightly increased (~1.5%) using linear DNA rather than a circular plasmid, pAHC25 (0.8%) (Tamás et al 2009). This result is in accordance with previously published efficiency data for biolistic transformation of wheat.…”

Section: Discussionmentioning

confidence: 99%

Generating Marker-Free Transgenic Wheat Using Minimal Gene Cassette and Cold-Inducible Cre/Lox System

et al. 2014

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Abstract:Abstract The precise elimination of selectable marker genes is highly desirable, when their function is no longer needed, because their presence raised worldwide public concerns against the release of genetically modified plants. This is the first report of simultaneous application of the minimal gene cassette and cold-inducible Cre/lox recombination system in wheat. The bar selection and cre-recombinase genes were eliminated from T0 and T1 transgenic lines with 44% and 51% efficiency. This approach provides a new, reasonably effective technique to produce selection genefree transgenic wheat lines either immediately after tissue culture, or from the subsequent transgenic generation. The advantage of this method is that it does not require any additional cold treatment during the plant regeneration/growing because the transgene elimination is ensured by the vernalisation. Application of this method prevents gene flow by pollen and seed, because the selection and recombinase genes are eliminated before pollen development, therefore reducing the risk of GM plants. Powered by Editorial Manager® and ProduXion Manager® from Aries Systems CorporationGenerating marker-free transgenic wheat using minimal gene cassette and cold inducible Cre/lox system AbstractThe precise elimination of selectable marker genes is highly desirable, when their function is no longer needed, because their presence raised worldwide public concerns against the release of genetically modified plants. This is the first report of simultaneous application of the minimal gene cassette and cold-inducible Cre/lox recombination system in wheat. The bar selection and cre-recombinase genes were eliminated from T 0 and T 1 transgenic lines with 44% and 51% efficiency. This approach provides a new, reasonably effective technique to produce selection gene-free transgenic wheat lines either immediately after tissue culture, or from the subsequent transgenic generation. The advantage of this method is that it does not require any additional cold treatment during the plant regeneration/growing because the transgene elimination is ensured by the vernalisation. Application of this method prevents gene flow by pollen and seed, because the selection and recombinase genes are eliminated before pollen development, therefore reducing the risk of GM plants.

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“…Transgenic maize seeds expressing the AH protein contained up to 32 % more protein than wild-type seeds and contained higher levels of lysine, tryptophan and isoleucine (Rascón-Cruz et al 2004). Similarly transgenic potato tubers expressing AH contained 45 % more protein than normal (Chakraborty et al 2000) and transgenic wheat seeds contained nearly 2.5 % AH as a proportion of total seed protein, increasing the levels of lysine to 6.4 % and tyrosine to 3.8 % (Tamás et al 2009). …”

Section: Essential Amino Acidsmentioning

confidence: 99%

The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints

et al. 2012

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Malnutrition is a prevalent and entrenched global socioeconomic challenge that reflects the combined impact of poverty, poor access to food, inefficient food distribution infrastructure, and an over-reliance on subsistence mono-agriculture. The dependence on staple cereals lacking many essential nutrients means that malnutrition is endemic in developing countries. Most individuals lack diverse diets and are therefore exposed to nutrient deficiencies. Plant biotechnology could play a major role in combating malnutrition through the engineering of nutritionally enhanced crops. In this article, we discuss different approaches that can enhance the nutritional content of staple crops by genetic engineering (GE) as well as the functionality and safety assessments required before nutritionally enhanced GE crops can be deployed in the field. We also consider major constraints that hinder the adoption of GE technology at different levels and suggest policies that could be adopted to accelerate the deployment of nutritionally enhanced GE crops within a multicomponent strategy to combat malnutrition.

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Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality

Cited by 63 publications

References 46 publications

Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber

Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber

Generating Marker-Free Transgenic Wheat Using Minimal Gene Cassette and Cold-Inducible Cre/Lox System

The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints

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