Transforming Growth Factor- 1 Upregulates the Tight Junction and P-glycoprotein of Brain Microvascular Endothelial Cells

Dohgu, Shinya; Yamauchi, Atsushi; Takata, Fuyuko; Naito, Mikihiko; Tsuruo, Takashi; Higuchi, Shun; Sawada, Yasufumi; Kataoka, Yasufumi

doi:10.1023/b:cemn.0000022776.47302.ce

Cited by 92 publications

(70 citation statements)

References 18 publications

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“…It was reported that expression of occuludin, one of the transmembrane tight junction proteins, was not changed by exposure of ECs to shear stress of 1.5 Pa (28) . However, it was showed that TGF-ß1 synthesized by SMCs upregulated tight junctions (29) and its synthesis in CM may be increased by cellular interactions with ECs under shear conditions. Both shear stress and cellular interactions between ECs and SMCs may induce the tight junction formations and lead to the reduction of the EC permeability in CM.…”

Section: Discussionmentioning

confidence: 99%

Effect of Shear Stress on Permeability of Vascular Endothelial Monolayer Cocultured with Smooth Muscle Cells

Sakamoto

Ohashi

Sato

2004

JSME Int. J., Ser. C

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Effect of fluid shear stress on permeability of endothelial monolayer was investigated using an endothelial cell (EC)-smooth muscle cell (SMC) cocultured model (CM). Permeability of ECs to bovine serum albumin was measured after exposure to shear stress of 1.5 Pa for 48 hours. Morphology and VE-cadherin expression of ECs in CM was almost same as of ECs cultured alone (monocultured model, MM). Under static condition, EC permeability was 5.1 ± 3.0 × 10 −6 cm/sec (mean ± SD) in MM and 6.5 ± 3.4 × 10 −6 cm/sec in CM. After exposure to shear stress, EC permeability in CM (2.2 ± 1.9 × 10 −6 cm/sec, p < 0.05) significantly decreased compared with the static model. However, EC permeability in MM (3.9±3.2×10 −6 cm/sec) did not significantly change compared with static cultured condition. These results suggested that cellular interactions between ECs and SMCs have important influences on EC permeability.

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Section: Discussionmentioning

confidence: 99%

Effect of Shear Stress on Permeability of Vascular Endothelial Monolayer Cocultured with Smooth Muscle Cells

Sakamoto

Ohashi

Sato

2004

JSME Int. J., Ser. C

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“…In the CNS, TGF-b is neuroprotective, and in vitro studies have shown its capacity to induce Mdr1 activity and to reduce BBB permeability (Dohgu et al 2004). TGF-b is secreted by astrocytes and CNS-ECs, and TGF-b known to downregulate the extent of leukocyte transmigration across the endothelium.…”

Section: Regulation Of the Bbb By Astrocytesmentioning

confidence: 99%

The Blood–Brain Barrier

Daneman

Prat

2015

Cold Spring Harb Perspect Biol

2,418

1,829

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Blood vessels are critical to deliver oxygen and nutrients to all of the tissues and organs throughout the body. The blood vessels that vascularize the central nervous system (CNS) possess unique properties, termed the blood-brain barrier, which allow these vessels to tightly regulate the movement of ions, molecules, and cells between the blood and the brain. This precise control of CNS homeostasis allows for proper neuronal function and also protects the neural tissue from toxins and pathogens, and alterations of these barrier properties are an important component of pathology and progression of different neurological diseases. The physiological barrier is coordinated by a series of physical, transport, and metabolic properties possessed by the endothelial cells (ECs) that form the walls of the blood vessels, and these properties are regulated by interactions with different vascular, immune, and neural cells. Understanding how these different cell populations interact to regulate the barrier properties is essential for understanding how the brain functions during health and disease.

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“…67 Some pericytes-derived mediators also exert a modulating influence on BBB function by regulating the expression of endothelial junction proteins. There include transforming growth factor-beta1 (TGF-beta1), 68 glial cell-derived neurotrophic factor (GDNF), 69 and angiopoietin 1 (ANG-1).…”

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confidence: 99%