Transformed tobacco plants with increased tolerance to drought

Bridging the Rice Yield Gaps under Drought: QTLs, Genes, and their Use in Breeding Programs

“…Transgenic approach is being practiced throughout the world to improve resistance to biotic stresses and tolerance of abiotic stresses in a number of crops. Rapid progress in recombinant-DNA technology and development of accurate and efficient gene-transfer protocols have resulted in efficient engineering of genes encoding compatible organic solutes [202], and biosynthesis of glycine betaine in tobacco and maize [203,204]; trehalose-6-phosphate synthase or phosphatase (TPSP) in rice [205], and tobacco [206,207]; choline dehydrogenase in maize [204]; and pyrroline-5-carboxylate synthetase (P5CS) in wheat [208], tobacco [209], soybeans [210], and petunias [211].…”

Effect of drought and combined drought and heat stress on polyamine metabolism in proline-over-producing tobacco plants

“…M51) and transgenic 35S:P5CSF129A tobacco plants (for detailed information see Ref. [15] were grown in soil in a growth chamber (SANYO MLR 350H, Osaka, Japan) for 6 weeks with a 16-h photoperiod at 130 mmol m À2 , day/ night temperatures of 25/23 C, and a relative humidity (RH) of ca. 80%.…”

“…In P5CS from Vigna aconitifolia, this was achieved by substituting the phenylalanine residue at position 129 with alanine. Using this construct, tobacco plants with significantly elevated proline levels were prepared [15].…”

Comparison of hormonal responses to heat, drought and combined stress in tobacco plants with elevated proline content

“…M51) wild-type (WT) and transgenic 35S:P5CSF129A plants (M51-1) (for characterization see Gubis et al, 2007) were grown in soil (pot size 300 mL) in a growth chamber (SANYO MLR 350H, Osaka, Japan) for 6 weeks on a 16-h photoperiod at 130 mol m −2 s −1 , at 25/23 • C and relative humidity (RH) at approximately 80%. Drought stress was imposed by the cessation of watering for 10 days at reduced RH (35%).…”

“…In order to compare stress tolerance of plants significantly differing in Pro content, the hormonal response was followed in WT and transformants with constitutively enhanced expression of a gene for Pro biosynthesis ( 2 -pyrroline-5-carboxylate synthetase, P5CS, EC 2.7.2.11/1.2.1.41). As the native P5CS is subjected to feed-back inhibition by the product (Pro), we used transformants prepared by Gubis et al (2007) with a modified construct (substitution of phenylalanine for alanine at position 129, Zhang et al, 1995), in which the feed-back inhibition was removed.…”