Transformation of the Osc3h52 Gene Promoter in Tissue Culture of Sugarcane
Abstract:Sugarcane growth is negatively affected by abiotic stresses. Genetic engineering of sugarcane with abiotic stressresponsive genes has been the method of choice to confer abiotic stress tolerance. In the present work, we optimized callus induction and transformation of the sugarcane variety CP-77-400 with the promoter region of the OsC3H52 gene to analyze its regulatory function under drought and salt stress. Calli were induced using different callus induction media (CIM). The promoter region of the OsC3H52 gen… Show more
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