Transformation and Transgenic Expression studies of Glyphosate tolerant and Cane Borer Resistance Genes in Sugarcane (&lt;i&gt;Sccharum officinarum&lt;/i&gt; L.)

Qamar, Zahida; Riaz, Saman; Nasir, Idrees Ahmad; Ali, Qurban; Husnain, Tayyab

doi:10.5376/mpb.2015.06.0012

Cited by 5 publications

(7 citation statements)

References 106 publications

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“…The Cry1Ac and Cry2A (Rao et al, 2011 ) plasmid along with the GTGene (Bajwa et al, 2013 ; Qamar et al, 2015a , b ) plasmid were transformed into competent DH5α cells using heat shock. After transformation, 1 ml of SOC solution or LB broth was added, and the culture was placed in a shaker for 1–2 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Genes of this sort are widely used to develop insect resistance in various crops (Gasser and Fraley, 1992 ; Ali et al, 2014 ). Among these genes, transgenic cotton that expresses insecticidal proteins from B. thuringiensis has been one of the most rapidly adopted GM crops in the world (Barwale et al, 2004 ; Dong et al, 2005 ; James, 2013 ; Qamar et al, 2015a , b ). Transgenic cotton contains one or more Cry genes, such as Cry1Ac, Cry1Ac, and Cry2Ab, or Cry1Ac and Cry1F.…”

Section: Introductionmentioning

confidence: 99%

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Transformation and Evaluation of Cry1Ac+Cry2A and GTGene in Gossypium hirsutum L.

et al. 2015

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More than 50 countries around the globe cultivate cotton on a large scale. It is a major cash crop of Pakistan and is considered “white gold” because it is highly important to the economy of Pakistan. In addition to its importance, cotton cultivation faces several problems, such as insect pests, weeds, and viruses. In the past, insects have been controlled by insecticides, but this method caused a severe loss to the economy. However, conventional breeding methods have provided considerable breakthroughs in the improvement of cotton, but it also has several limitations. In comparison with conventional methods, biotechnology has the potential to create genetically modified plants that are environmentally safe and economically viable. In this study, a local cotton variety VH 289 was transformed with two Bt genes (Cry1Ac and Cry2A) and a herbicide resistant gene (cp4 EPSPS) using the Agrobacterium mediated transformation method. The constitutive CaMV 35S promoter was attached to the genes taken from Bacillus thuringiensis (Bt) and to an herbicide resistant gene during cloning, and this promoter was used for the expression of the genes in cotton plants. This construct was used to develop the Glyphosate Tolerance Gene (GTGene) for herbicide tolerance and insecticidal gene (Cry1Ac and Cry2A) for insect tolerance in the cotton variety VH 289. The transgenic cotton variety performed 85% better compared with the non-transgenic variety. The study results suggest that farmers should use the transgenic cotton variety for general cultivation to improve the production of cotton.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transformation and Evaluation of Cry1Ac+Cry2A and GTGene in Gossypium hirsutum L.

et al. 2015

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“…However the number of PCR cycles and extent of decrease in temperature across each cycle was varied for better amplifications. TD-PCR amplification greatly helped in avoiding spurious smaller fragments which often resulted in target gene amplification of complex genomes like sugarcane during the PCR cycles (DON et al, 1991;QAMAR et al, 2015;NAZAR et al, 2017;QAMAR et al, 2017;AWAIS et al, 2019). Sugarcane yield depends upon various agro-morphological traits, viz., stalk number, cane height, cane girth and sugar recovery.…”

Section: Discussionmentioning

confidence: 99%

Identification of novel QTLs controlling sugarcane smut resistance and yield traits

Ahmad¹,

Hm²,

Ali³

et al. 2019

Genetika

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“…Although Cry and Vip toxins have the same general mode of action, they have no structural homology and bind to different sites in the larval midgut, which suggests that strong crossresistance between them is highly unlikely (Carrière et al, 2016;Chakroun et al, 2016). Crops genetically engineered to produce insecticidal proteins from Bt have revolutionized control of some major lepidopteran and coleopteran pests in important crops such as corn and cotton (Tabashnik et al, 2020), but even more significantly the development of Bt crops has been extremely important in dramatically reducing the use of harmful chemical insecticides in agriculture (Qamar et al, 2015).…”

Section: Resistance To Pestsmentioning

confidence: 99%

Genetic Transformation of Sugarcane, Current Status and Future Prospects

et al. 2021

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Sugarcane (Saccharum spp.) is a tropical and sub-tropical, vegetative-propagated crop that contributes to approximately 80% of the sugar and 40% of the world’s biofuel production. Modern sugarcane cultivars are highly polyploid and aneuploid hybrids with extremely large genomes (>10 Gigabases), that have originated from artificial crosses between the two species, Saccharum officinarum and S. spontaneum. The genetic complexity and low fertility of sugarcane under natural growing conditions make traditional breeding improvement extremely laborious, costly and time-consuming. This, together with its vegetative propagation, which allows for stable transfer and multiplication of transgenes, make sugarcane a good candidate for crop improvement through genetic engineering. Genetic transformation has the potential to improve economically important properties in sugarcane as well as diversify sugarcane beyond traditional applications, such as sucrose production. Traits such as herbicide, disease and insect resistance, improved tolerance to cold, salt and drought and accumulation of sugar and biomass have been some of the areas of interest as far as the application of transgenic sugarcane is concerned. Although there have been much interest in developing transgenic sugarcane there are only three officially approved varieties for commercialization, all of them expressing insect-resistance and recently released in Brazil. Since the early 1990’s, different genetic transformation systems have been successfully developed in sugarcane, including electroporation, Agrobacterium tumefaciens and biobalistics. However, genetic transformation of sugarcane is a very laborious process, which relies heavily on intensive and sophisticated tissue culture and plant generation procedures that must be optimized for each new genotype to be transformed. Therefore, it remains a great technical challenge to develop an efficient transformation protocol for any sugarcane variety that has not been previously transformed. Additionally, once a transgenic event is obtained, molecular studies required for a commercial release by regulatory authorities, which include transgene insertion site, number of transgenes and gene expression levels, are all hindered by the genomic complexity and the lack of a complete sequenced reference genome for this crop. The objective of this review is to summarize current techniques and state of the art in sugarcane transformation and provide information on existing and future sugarcane improvement by genetic engineering.

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Transformation and Transgenic Expression studies of Glyphosate tolerant and Cane Borer Resistance Genes in Sugarcane (<i>Sccharum officinarum</i> L.)

Cited by 5 publications

References 106 publications

Transformation and Evaluation of Cry1Ac+Cry2A and GTGene in Gossypium hirsutum L.

Transformation and Evaluation of Cry1Ac+Cry2A and GTGene in Gossypium hirsutum L.

Identification of novel QTLs controlling sugarcane smut resistance and yield traits

Genetic Transformation of Sugarcane, Current Status and Future Prospects

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