Transformation and Cotransformation of<i>Gaeumannomyces graminis</i>to Phleomycin Resistance

Pilgeram, Alice L.; Henson, Joan M.

doi:10.1094/phyto-80-1124

Cited by 15 publications

(12 citation statements)

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“…All published reports of transformation of G. graminis have used PEG‐CaCl 2 ‐mediated protoplast transformation. Benomyl‐ and phleomycin‐resistant transformants of Ggg and Ggt have been obtained using this method, but the transformation efficiency is very low (1–5 transformants/µg DNA per 10 7 protoplasts) (Bowyer et al ., 1995; Henson et al ., 1988; Pilgeram and Henson, 1990), and varies greatly from experiment to experiment (P. Bowyer, personal communication). The stability of the integrated DNA varies.…”

Section: Genetics and Transformation Of G Graminismentioning

confidence: 99%

“…The stability of the integrated DNA varies. Transgenes in the majority of transformants are mitotically stable, but their stability through meiosis varies with copy number and with different genes (Pilgeram and Henson, 1990, 1992; Pilgeram et al ., 1993). For example, benomyl‐ and phleomycin‐resistant transformants of Ggt and Ggg were mitotically stable irrespective of transgene copy number.…”

Section: Genetics and Transformation Of G Graminismentioning

confidence: 99%

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Gaeumannomyces graminis, the take‐all fungus and its relatives

Freeman

Ward

2004

Molecular Plant Pathology

100

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SUMMARY Take‐all, caused by the fungus Gaeumannomyces graminis var. tritici, is the most important root disease of wheat worldwide. Many years of intensive research, reflected by the large volume of literature on take‐all, has led to a considerable degree of understanding of many aspects of the disease. However, effective and economic control of the disease remains difficult. The application of molecular techniques to study G. graminis and related fungi has resulted in some significant advances, particularly in the development of improved methods for identification and in elucidating the role of the enzyme avenacinase as a pathogenicity determinant in the closely related oat take‐all fungus (G. graminis var. avenae). Some progress in identifying other factors that may be involved in determining host range and pathogenicity has been made, despite the difficulties of performing genetic analyses and the lack of a reliable transformation system.

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Section: Genetics and Transformation Of G Graminismentioning

confidence: 99%

Section: Genetics and Transformation Of G Graminismentioning

confidence: 99%

Gaeumannomyces graminis, the take‐all fungus and its relatives

Freeman

Ward

2004

Molecular Plant Pathology

100

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“…tritici (strain DM528) and G. graminis var. graminis (strain DM562) were transformed to benomyl resistance or phleomycin resistance as described ( [13,14]; Table 1). G. graminis was routinely cultured on complex L medium [15] at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…Fungal DNA extractions, electrophoresis and hybridization of dried gels were performed as described [13,14]. MspI and HpaII digested DNA was transferred to Zeta-Probe nylon membranes and hybridizations were conducted according to the manufacturer's recommendations (BioRad Laboratories, Richmond, CA).…”

Section: Isolation and Analysis Of Dnamentioning

confidence: 99%

“…If RIP occurs, however, it is not extensive, as many repeated sequences survive meiosis and/or can be reactivated. Initial investigation of fungicide resistance genes in G. graminis transformants indicated that although two transformants lost their drug resistance phenotypes after mitotic growth on host plants, transforming DNA was otherwise stable mitotically and meiotically [13,14]. However, only a few ascospore progeny of multi-copy PhleoR G.…”

Section: Introductionmentioning

confidence: 99%

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The fate of integrated DNA in Gaeumannomyces graminis transformants

Pilgeram¹

1993

FEMS Microbiology Letters

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The fate of integrated transforming DNA in fungicide (benomyl or phleomycin)-resistant transformants of Gaeumannomyces graminis was investigated. Transforming DNA was mitotically and meiotically stable in several transformants. However, other transformants of varieties graminis and tritici were less resistant to fungicide after meiosis. Hybridization analyses indicated that transforming DNA in ascospore progeny had rearrangements and/or deletions, and that similar events could occur during a second meiotic cycle, further reducing their levels of resistance. Rearrangements and/or deletions were most common in transforrnants with multiple copies of a benomyl-resistance (Ben r) plasmid. Transforming sequences were not extensively methylated, even when present in multiple copies.

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Genetic Manipulation of Fungi by DNA-Mediated Transformation

Lemke

Peng

1995

Genetics and Biotechnology

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Transformation and Cotransformation ofGaeumannomyces graministo Phleomycin Resistance

Cited by 15 publications

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Gaeumannomyces graminis, the take‐all fungus and its relatives

Gaeumannomyces graminis, the take‐all fungus and its relatives

The fate of integrated DNA in Gaeumannomyces graminis transformants

Genetic Manipulation of Fungi by DNA-Mediated Transformation

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