Transfer RNA genes in the cap-oxil region of yeast mitochondrial DNA

Berlani, Roberta E.; Bonitz, S G; Coruzzi, Gloria M.; Nóbrega, Marina P.; Tzagoloff, Alexander

doi:10.1093/nar/8.21.5017

Cited by 35 publications

(15 citation statements)

References 40 publications

(43 reference statements)

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“…The following strains were used: the rho+ strain MH41-7B and its rho mutants Fll and CEP2 (2) were kindly given by Prof. H. Fukuhara; the rho strain DS502 (6)(7)(8) was kindly given by Prof. A. Tzagaloff. The regions of the mitochondrial genome retained by the mutants are reported in Fig.…”

Section: Materials and Methods Strains And Culture Conditionsmentioning

confidence: 99%

“…Since the sequence of mtDNA from strain DS502 is known (6)(7)(8), it was possible to align the S1 resistent DNA fragments with the DNA sequence and thus define precisely the 5' termini of the transcripts having a size smaller than that of the probes.…”

Section: Identification Of Transcripts Carrying the Sequences Of Trnamentioning

confidence: 99%

“…In mitochondrial DNA from S.cerevisiae the genes for tRNA (except tRNAtrp, tRNA2er and tRNA91u) are organized in several clusters in the region between the two ribosomal RNA genes (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). The region containing the clusters of tRNA genes has been extensively sequenced (3)(4)(5)(6)(7)(8)(9)(10)(11) but little information is available concerning its transcription.…”

Section: Introductionmentioning

confidence: 99%

“…The region containing the clusters of tRNA genes has been extensively sequenced (3)(4)(5)(6)(7)(8)(9)(10)(11) but little information is available concerning its transcription. The analysis of the flanking sequences has actually not revealed evident promotor-like structures (3).…”

Section: Introductionmentioning

confidence: 99%

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Transcripts of mitochondrial tRNA genes inSaccharomyces cerevisiae

1982

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The transcription of a group of tRNA genes from the large tRNA gene cluster of mitochondrial DNA from Saccharomyces cerevisiae hase been investi gated by hybridization with DNA probes carrying t coding sequences and small portions of the A+T rich intergenic regions.Results have shown that in some rho mutants (DS502, Fll) mature tRNA was absent, but a few transcripts could be detected. Some high molecular weight species actually hybridized with DNA probes carrying different tRNA coding sequences. Low molecular weight transcripts (100-150 nucleotides, carrying one tRNA sequence) were also present in these mutants. A high molecular weight transcript was also observed in the wild type, though in much more 1i mited amount. The low molecular weight transcripts were analysed by the Sl mapping technique and found to include both a tRNA sequence and the upstream 5' flanking region extending as far as the 3' end of the preceding tRNA gene.The results suggest the existence of a common transcript bearing sever al tRNA sequences and indicate a possible mechanism of processing, which might be defective in mutants.

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Section: Materials and Methods Strains And Culture Conditionsmentioning

confidence: 99%

Section: Identification Of Transcripts Carrying the Sequences Of Trnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcripts of mitochondrial tRNA genes inSaccharomyces cerevisiae

1982

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“…In particular, 16 tRNA genes are clustered in a 9-kbp region between the 21S rRNA and oxiI genes; no other genes are present in this region. This region has been almost completely sequenced (Berlani et al, 1980a(Berlani et al, , 1980bBonitz and Tzagoloff, 1980), but little is known about its transcription. The organization of these tRNA genes is similar to that observed in the mitochondrial genomes of other lower fungi (Heckman et al, 1979;Kochel et al, 1981) and very different from that present in mammalian mitochondrial DNA (mtDNA), where tRNA genes are interposed between protein genes (Montoya et al, 1981(Montoya et al, , 1982.…”

Section: Introductionmentioning

confidence: 99%

Expression of the clustered mitochondrial tRNA genes in Saccharomyces cerevisiae: transcription and processing of transcripts.

Palleschi¹,

Francisci²,

Zennaro³

et al. 1984

The EMBO Journal

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The transcripts of a cluster of eight tRNA genes localized in the Cap-oxiI region of the mitochondrial genome of Saccharomyces cerevisiae were investigated by hybridization of gene-specific probes on Northern blots of mitochondrial RNA and by SI mapping of the 5' termini of the transcripts. Two rho-mutants that lack mature tRNA species and accumulate precursors have been used to detect transcripts that are not detectable in wild-type (w.t.) mitochondria. The results have shown the existence of polygenic transcripts carrying at least 5-7 tRNA sequences, both in w.t. and in rhostrains. The existence of several alternative processing pathways, which involve cleavage at the 3' and 5' ends of the tRNA sequences and in the long intergenic regions (possibly at GC clusters), is suggested. Cleavage at the 5' ends of tRNA sequences is defective in the mutant strains. The transcripts of the genes for tRNATZN and tRNACys (the tRNA genes immediately downstream from the 21S rRNA gene) have been analyzed; the possibility that these species represent primary transcripts is considered, and potential sites for initiation of transcription of the clustered tRNA genes are discussed.

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Location of transcriptional control signals and transfer RNA sequences in Torulopsis glabrata mitochondrial DNA.

Clark-Walker¹,

McArthur²,

Sriprakash³

1985

The EMBO Journal

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Determination of sequences from the nine regions separating the large genes in the 19‐kbp mitochondrial DNA from Torulopsis glabrata has led to the identification of 23 tRNA genes and to the recognition of two types of short repeated sequence implicated in mitochondrial genome expression. The two short repeated sequences are a nonanucleotide motif, 5′‐TATAAGTAA‐3′ and a dodecanucleotide motif, 5′‐TATAATATTCTT‐3′. By RNA sequence determination it has been found that primary transcripts of the small and large rRNAs commence at the 3′ penultimate adenine of the nonanucleotide sequence. This motif has also been found in the DNA sequence upstream from f‐methionine, phenylalanine, leucine, tyrosine and glycine tRNAs, cytochrome oxidase subunit 2 and ATPase subunit 9. The dodecanucleotide sequence is found at least once in each of the nine regions between the large genes. Determination of the 3′ ends of the small and large rRNAs has shown their location to be 8 and 23 nucleotides downstream from the dodecanucleotide sequence. This motif is thought to be involved in signalling processing of polycistronic transcripts. Such transcripts are invoked to account for the production of mRNAs for cytochrome b, cytochrome oxidase subunits 1 and 3, and the joint mRNA for ATPase subunits 8 and 6 genes that lack an adjacent upstream nonanucleotide transcription initiation signal sequence. Processing of polycistronic transcripts at tRNA sequences is also implicated in the formation of mature mRNAs. From the position of tRNA genes relative to the nonanucleotide motif it appears that clusters of these genes are co‐transcribed with downstream sequences for cytochrome oxidase subunits 1 and 3.

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Transfer RNA genes in the cap-oxil region of yeast mitochondrial DNA

Cited by 35 publications

References 40 publications

Transcripts of mitochondrial tRNA genes inSaccharomyces cerevisiae

Transcripts of mitochondrial tRNA genes inSaccharomyces cerevisiae

Expression of the clustered mitochondrial tRNA genes in Saccharomyces cerevisiae: transcription and processing of transcripts.

Location of transcriptional control signals and transfer RNA sequences in Torulopsis glabrata mitochondrial DNA.

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