Transcriptomic response of the intertidal limpet Patella vulgata to temperature extremes

Moreira, Catarina; Stillman, Jonathon H.; Lima, Fernando P.; Xavier, Raquel; Seabra, Rui; Gomes, Filipe Veloso; Veríssimo, Ana; Silva, Sofia

doi:10.1016/j.jtherbio.2021.103096

Cited by 6 publications

(2 citation statements)

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“…Hnrnpd was downregulated after heat stress in this study, as was found in limpet foot tissue [ 17 ]. The hnRNP D0 encoded by Hnrnpd serves as a key factor involved in mRNA decay, and there exists a significant difference among the stabilizing effects of the four isoforms [ 88 ].…”

Section: Discussionsupporting

confidence: 72%

“…The THI reflects the heat stress of a population from an environmental perspective, but at an individual level, few accurate markers have been used in the prediction of heat stress, which may be largely related to the fact that the molecular mechanism regulating heat stress is not clear. With the Genes 2022, 13, 358 2 of 16 development of next-generation sequencing technology, numerous studies (including our previous studies) have identified lots of heat stress-related genes using RNA-sequencing (RNA-Seq) [12][13][14][15][16][17], but transcriptome information is still incomplete. A comprehensive analysis of alternative splicing of transcripts is lacking.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Genomic Alternative Splicing Patterns in Rat under Heat Stress Based on RNA-Seq Data

Huang

Dou

et al. 2022

Genes

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Heat stress is one of the most severe challenges faced in livestock production in summer. Alternative splicing as an important post-transcriptional regulation is rarely studied in heat-stressed animals. Here, we performed and analyzed RNA-sequencing assays on the liver of Sprague-Dawley rats in control (22 °C, n = 5) and heat stress (4 °C for 120 min, H120; n = 5) groups, resulting in the identification of 636 differentially expressed genes. Identification analysis of the alternative splicing events revealed that heat stress-induced alternative splicing events increased by 20.18%. Compared with other types of alternative splicing events, the alternative start increased the most (43.40%) after heat stress. Twenty-eight genes were differentially alternatively spliced (DAS) between the control and H120 groups, among which Acly, Hnrnpd and mir3064 were also differentially expressed. For DAS genes, Srebf1, Shc1, Srsf5 and Ensa were associated with insulin, while Cast, Srebf1, Tmem33, Tor1aip2, Slc39a7 and Sqstm1 were enriched in the composition of the endoplasmic reticulum. In summary, our study conducts a comprehensive profile of alternative splicing in heat-stressed rats, indicating that alternative splicing is one of the molecular mechanisms of heat stress response in mammals and providing reference data for research on heat tolerance in mammalian livestock.

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Section: Discussionsupporting

confidence: 72%