Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical gamma-oryzanol

Abstract

BackgroundAdult skeletal muscle myogenesis depends on the activation of satellite cells that have the potential to differentiate into new fibers. Gamma-oryzanol (GO), a commercially available nutriactive phytochemical, has gained global interest on account of its muscle-building and regenerating effects. Here, we investigated GO for its potential influence on myogenesis, using equine satellite cell culture model, since the horse is a unique animal, bred and exercised for competitive sport. To our knowledge, this is the first report where the global gene expression in cultured equine satellite cells has been described.MethodsEquine satellite cells were isolated from semitendinosus muscle and cultured until the second day of differentiation. Differentiating cells were incubated with GO for the next 24 h. Subsequently, total RNA from GO-treated and control cells was isolated, amplified, labeled, and hybridized to two-color Horse Gene Expression Microarray slides. Quantitative PCR was used for the validation of microarray data.ResultsOur results revealed 58 genes with changed expression in GO-treated vs. control cells. Analysis of expression changes suggests that various processes are reinforced by GO in differentiating equine satellite cells, including inhibition of myoblast differentiation, increased proliferation and differentiation, stress response, and increased myogenic lineage commitment.ConclusionsThe present study may confirm putative muscle-enhancing abilities of GO; however, the collective role of GO in skeletal myogenesis remains equivocal. The diversity of these changes is likely due to heterogenous growth rate of cells in primary culture. Genes identified in our study, modulated by the presence of GO, may become potential targets of future research investigating impact of this supplement in skeletal muscle on proteomic and biochemical level.Electronic supplementary materialThe online version of this article (doi:10.1186/s12263-016-0523-5) contains supplementary material, which is available to authorized users.

Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide

“…GO is known to affect gene expression and several metabolic pathways related to inflammation [11,12] and oxidative stress [13]. Szczesniak et al [14] demonstrated that GO may affect different processes in differentiating equine satellite cells (ESCs), including inhibition of myoblast differentiation, and increased proliferation and differentiation.…”

Transcriptomic Profile of Primary Culture of Skeletal Muscle Cells Isolated from Semitendinosus Muscle of Beef and Dairy Bulls

“…The mRNA sequences of genes were obtained from the NCBI Nucleotide database. Primer sequences were designed using the Primer3 ( ) and Primer-Blast software ( ) and then checked using Oligo Calculator ( ) as described previously [ 84 ]. Primer sequences, annealing temperatures, and product length measurements are listed in Table S4 .…”

Transcriptomic profile of semitendinosus muscle of bulls of different breed and performance

“…The mRNA sequences of the genes selected for validation were obtained from the NCBI Nucleotide database. Primer sequences were designed using the Primer3 and Primer-Blast software (NCBI database) and then checked using Oligo Calculator as described previously (Szcześniak et al 2016 ). The sequences of the designed primers are listed in Supplementary Table 1 .…”