2018
DOI: 10.3390/toxins10120544
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Abstract: Venomous animals are found through a wide taxonomic range including cartilaginous fish such as the freshwater stingray Potamotrygon motoro occurring in South America, which can injure people and cause venom-related symptoms. Ensuring the efficacy of drug development to treat stingray injuries can be assisted by the knowledge of the venom composition. Here we performed a detailed transcriptomic characterization of the venom gland of the South American freshwater stingray Potamotrygon motoro. The transcripts ret… Show more

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Cited by 13 publications
(13 citation statements)
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“…Accordingly, we identified serine proteases and metalloproteinases in all our transcriptome datasets, supporting the bioactivity data and providing the basis for the hemostasis cluster (Figure A2). The abundance of PLA2 transcripts also agrees with the reanalysis (for comparability) of the Potamotrygon transcriptomes reported previously (Datasets S6-S8) [29,30] and corresponding enzymes have been identified in nearly all animal venoms analyzed thus far. However, only minimal PLA2 activity was found in stingray venom in one previous study [26], and another study claimed that PLA2 has never been detected in fish venom [21].…”
Section: Discussionsupporting
confidence: 88%
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“…Accordingly, we identified serine proteases and metalloproteinases in all our transcriptome datasets, supporting the bioactivity data and providing the basis for the hemostasis cluster (Figure A2). The abundance of PLA2 transcripts also agrees with the reanalysis (for comparability) of the Potamotrygon transcriptomes reported previously (Datasets S6-S8) [29,30] and corresponding enzymes have been identified in nearly all animal venoms analyzed thus far. However, only minimal PLA2 activity was found in stingray venom in one previous study [26], and another study claimed that PLA2 has never been detected in fish venom [21].…”
Section: Discussionsupporting
confidence: 88%
“…Our transcriptome analysis revealed several intriguing results. First, the augerpeptide was present in all of our transcriptome datasets and was often the most abundant transcript, supporting its detection in the first P. motoro transcriptome study [30]. However, the activity of this peptide remains unknown.…”
Section: Discussionsupporting
confidence: 74%
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“…The current gold standard for whole venom gland RNA-seq has been Illumina HiSeq sequencing, using Trinity software to generate a de novo assembly and Trinotate software to annotate the assembly [ 1 , 2 , 3 , 99 , 100 , 101 ]. This will certainly change as long-read RNA-seq techniques are refined and upgraded.…”
Section: Techniques In Venomicsmentioning
confidence: 99%
“…Recently, transcriptomic methods combined with proteomic data have been used to analyse the venoms from animals such as snakes, spiders, and cone snails [22]. However, at this point fewer than ten venomous fish species have undergone transcriptomic analysis, and only three of these integrated with proteomic data to evaluate venom composition [23][24][25][26][27][28]. Those with corresponding proteomic data include a ray species [26], one catfish species [24], and a fang blenny species.…”
Section: Accepted Manuscriptmentioning
confidence: 99%