Transcriptomic analysis of the orchestrated molecular mechanisms underlying fruiting body initiation in Chinese cordyceps

Transcriptome and metabonomics combined analysis revealed the energy supply mechanism involved in fruiting body initiation in Chinese cordyceps

“…As reported recently, primary and secondary metabolites such as sugars, bioactive substance were vital components that determined the initiation of fruiting body 1 , 4 . To further explore the differentially abundant metabolites in different groups, hierarchical clustering was applied to select DEMs and the change patterns of which were displayed in the heatmap (Fig.…”

“…Subsequently, our team found that VeA and Hsp70 genes were specifically expressed during the fruiting body initiation of Cordyceps cordyceps 8 . As outlined recently, Chinese cordyceps contained six MYB transcription factors, among which expression of MYB-3 was the highest at the maturation stage of its fruiting body, suggesting that MYB-3 might be involved in its sexual development 1 . Although so much efforts have been made to explore regulatory genes related to fruiting body initiation, the key gene for triggering this process was still unknown.…”

“…Materials S1 (pre-primordium) batmoth larva inoculated with Ophiocordyceps sinensis ( O. sinensis ) were provided by Hangzhou Mingxu Biotechnology Co., LTD. Materials were cultured as described by Zhao et al with some changes 1 . When the Chinese cordyceps reached at the beginning of germination stage (about 30 days), they were randomly and equally divided into two groups: one group collected as materials (primordium germination), another group continued to culture until primordium formated (55 days), these samples as materials (after-primordium), 100 mg was taken from each of the 3 samples at different stages, and 3 biological replicates were taken from each group, all samples were immediately frozen in liquid nitrogen and stored at − 80 °C.…”

“…The Chinese cordyceps, one of the precious traditional herbal medicines in China, is a complex of batmoth larvae with Ophiocordyceps sinensis ( O. sinensis ), used for health care and cure diseases and even other medical effects 1 . It is extremely expensive for its medicinal benefits and decreasing supply.…”

Stable reference gene selection for Ophiocordyceps sinensis gene expression studies under different developmental stages and light-induced conditions

“…Through transcriptome and proteome analyses, many differentially expressed genes were identified in different stages of fruiting body development [ 12 ]. These genes are involved in MAPK signal transduction, glucose metabolism [ 13 ], amino acid metabolism [ 14 ], lipid metabolism, and other pathways [ 15 , 16 ]. Simultaneously, it was also found that O .…”