Transcriptome sequencing reveals fibrotic associated-genes involved in bovine mammary fibroblasts with Staphylococcus aureus

Miao, Zengqiang; Ding, Ye; Zhao, Nan; Chen, Xunan; Cheng, Hui-Xin; Wang, Jinling; Liu, Yonghong; Wang, Fenglong

doi:10.1016/j.biocel.2020.105696

Cited by 3 publications

(2 citation statements)

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“…The expression of NFKBIA significantly increased in staphylococci- and in the mammary gland parenchyma of E. coli -infected cow ( 18 , 66 ) or in the bovine mammary epithelial cells ( 9 ). But the expression level of NFKBIA did not significantly alter in blood monocyte-derived macrophages infected with S. agalactiae ( 67 ), blood leukocytes infected with Staphylococcus aureus ( 68 ) and E. coli ( 69 ), circulating leukocytes with clinical or subclinical mastitis in the UK ( 25 ), and the Chinese Holstein mammary fibroblasts infected with S. aureus ( 70 ). Meanwhile, the expression level of CXCL8, CCL5, IL6, CXCL3, IL1A, NOD2, TNF, CCL2, IL17A, IL10 , and CCL20 were significantly upregulated and showed a strong inflammatory response in these pathological tissues and cells, especially in monocyte-derived macrophages ( 67 ).…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Transcriptome mRNA and miRNA Profiles Reveals Self-Protective Mechanism of Bovine MECs Induced by LPS

Chen

Liu

et al. 2022

Front. Vet. Sci.

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Many studies have investigated the molecular crosstalk between mastitis-pathogens and cows by either miRNA or mRNA profiles. Here, we employed both miRNA and mRNA profiles to understand the mechanisms of the response of bovine mammary epithelial cells (bMECs) to lipopolysaccharide (LPS) by RNA-Seq. The total expression level of miRNAs increased while mRNAs reduced after LPS treatment. About 41 differentially expressed mRNAs and 45 differentially expressed miRNAs involved in inflammation were screened out. We found the NFκB-dependent chemokine, CXCL1, CXCL3, CXCL6, IL8, and CX3CL1 to be strongly induced. The anti-apoptosis was active because BCL2A1 and BIRC3 significantly increased with a higher expression. The effects of anti-microbe and inflammation were weakly activated because TNF, IL1, CCL20, CFB, S100A, MMP9, and NOS2A significantly increased but with a low expression, IL6 and β-defensin decreased. These activities were supervised by the NFKBIA to avoid excessive damage to bMECs. The bta-let-7a-5p, bta-miR-30a-5p, bta-miR-125b, and bta-miR-100 were essential to regulate infection process in bMECs after LPS induction. Moreover, the lactation potential of bMECs was undermined due to significantly downregulated SOSTDC1, WNT7B, MSX1, and bta-miR-2425-5p. In summary, bMECs may not be good at going head-to-head with the pathogens; they seem to be mainly charged with sending out signals for help and anti-apoptosis for maintaining lives after LPS induction.

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Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Transcriptome mRNA and miRNA Profiles Reveals Self-Protective Mechanism of Bovine MECs Induced by LPS

Chen

Liu

et al. 2022

Front. Vet. Sci.

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“…Additionally, similarities and differences exist in the immune-related pathways involved in host interactions between Gram-positive and -negative bacteria. For example, to explore a possible mechanism through which S. aureus damages bovine mammary glands, bovine mammary fibroblasts were infected with S. aureus , and the enriched DEGs involved in cytokine-cytokine receptor interactions, the MAPK signaling pathway, and the TNF signaling pathway, including the IL-17 and Nod-like receptor signaling pathways, were upregulated [ 22 ]. In a study of mouse lungs infected with P. multocida , the Toll-like receptor signaling pathway, cytokine-cytokine receptor interactions, the nucellar factor kappa b (NF-κB) signaling pathway, and the Nod-like receptor signaling pathway were significantly up-regulated [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Up-Regulation of Interleukin-10 in Splenic Immune Response Induced by Serotype A Pasteurellamultocida

Cheng

et al. 2022

Genes

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Pasteurella multocida (P. multocida) is an opportunistic pathogen that is common in livestock and poultry and leads to massive economic losses in the animal husbandry sector. In this study, we challenged mice with P. multocida strain HN02 by intraperitoneal injection and collected spleens to measure bacterial loads. We also performed histopathological analysis by hematoxylin and eosin (H&E) staining. Then we used RNA-sequencing (RNA-seq) to detect the mRNA expression levels in the mouse spleen and quantitative real-time PCR (qRT-PCR) to verify the sequencing data. Finally, we examined the effect of HN02 on anti-inflammatory cytokine interleukin-10 (IL-10) protein expression in the spleen through immunohistochemical analysis. The results showed that compared to those in the control group, the mouse spleens in the challenge group had lesions, and the average bacteria loads was (3.07 ± 1.09) × 106 CFU (colony-forming unit)/g. The RNA-seq results determined 3653 differentially expressed genes (DEGs), and the qRT-PCR analysis revealed immune-related genes consistent with the expression trend in the sequencing data. The number and area of IL-10 positive cells substantially increased to resist inflammation in the challenge group. In conclusion, we analyzed the spleens of mice infected with P. multocida from multiple perspectives, and our findings lay a foundation for subsequent studies on the mechanism of pathogen-host interactions.

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Identification of tumor mutation burden-associated molecular and clinical features in cancer by analyzing multi-omics data

Gao

Wang

2023

Front. Immunol.

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BackgroundTumor mutation burden (TMB) has been recognized as a predictive biomarker for immunotherapy response in cancer. Systematic identification of molecular features correlated with TMB is significant, although such investigation remains insufficient.MethodsWe analyzed associations of somatic mutations, pathways, protein expression, microRNAs (miRNAs), long non-coding RNAs (lncRNAs), competing endogenous RNA (ceRNA) antitumor immune signatures, and clinical features with TMB in various cancers using multi-omics datasets from The Cancer Genome Atlas (TCGA) program and datasets for cancer cohorts receiving the immune checkpoint blockade therapy.ResultsAmong the 32 TCGA cancer types, melanoma harbored the highest percentage of high-TMB (≥ 10/Mb) cancers (49.4%), followed by lung adenocarcinoma (36.9%) and lung squamous cell carcinoma (28.1%). Three hundred seventy-six genes had significant correlations of their mutations with increased TMB in various cancers, including 11 genes (ARID1A, ARID1B, BRIP1, NOTCH2, NOTCH4, EPHA5, ROS1, FAT1, SPEN, NSD1,and PTPRT) with the characteristic of their mutations associated with a favorable response to immunotherapy. Based on the mutation profiles in three genes (ROS1, SPEN, and PTPRT), we defined the TMB prognostic score that could predict cancer survival prognosis in the immunotherapy setting but not in the non-immunotherapy setting. It suggests that the TMB prognostic score’s ability to predict cancer prognosis is associated with the positive correlation between immunotherapy response and TMB. Nine cancer-associated pathways correlated positively with TMB in various cancers, including nucleotide excision repair, DNA replication, homologous recombination, base excision repair, mismatch repair, cell cycle, spliceosome, proteasome, and RNA degradation. In contrast, seven pathways correlated inversely with TMB in multiple cancers, including Wnt, Hedgehog, PI3K-AKT, MAPK, neurotrophin, axon guidance, and pathways in cancer. High-TMB cancers displayed higher levels of antitumor immune signatures and PD-L1 expression than low-TMB cancers in diverse cancers. The association between TMB and survival prognosis was positive in bladder, gastric, and endometrial cancers and negative in liver and head and neck cancers. TMB also showed significant associations with age, gender, height, weight, smoking, and race in certain cohorts.ConclusionsThe molecular and clinical features significantly associated with TMB could be valuable predictors for TMB and immunotherapy response and therefore have potential clinical values for cancer management.

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Transcriptome sequencing reveals fibrotic associated-genes involved in bovine mammary fibroblasts with Staphylococcus aureus

Cited by 3 publications

References 37 publications

Integrated Analysis of Transcriptome mRNA and miRNA Profiles Reveals Self-Protective Mechanism of Bovine MECs Induced by LPS

Integrated Analysis of Transcriptome mRNA and miRNA Profiles Reveals Self-Protective Mechanism of Bovine MECs Induced by LPS

Up-Regulation of Interleukin-10 in Splenic Immune Response Induced by Serotype A Pasteurellamultocida

Identification of tumor mutation burden-associated molecular and clinical features in cancer by analyzing multi-omics data

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