Transcriptome analysis reveals differentially expressed genes and pathways for oviduct development and defense in prelaying and laying hens

Yin, Lingqian; Yu, Ling; Zhang, Long; Ran, Jin-Shan; Li, Jingjing; Yang, Chaowu; Jiang, Xiaosong; Du, Huarui; Hu, Xiaofang; Liu, Yiping

doi:10.1111/aji.13159

Cited by 15 publications

(17 citation statements)

References 51 publications

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“…At present, a number of studies are available regarding the molecular mechanisms behind the transcription level changes in the magnum during the different reproductive stages (Jeong et al, 2013;Khan et al, 2019;Z. Yin, Lian, et al, 2019;L. Yin, Yu, et al, 2019); however, little information is available regarding the molecular mechanisms behind the cellular changes in the bird magnum during the whole laying cycle.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analyses to reveal the dynamic change mechanism of pigeon magnum during one egg‐laying cycle

Liu

et al. 2020

Molecular Reproduction Devel

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We analyzed the transcriptome of pigeon magnum in three stages (C1: preovulation, C2: post-ovulation, C3: 5-6 days after ovulation) to elucidate the molecular and cellular events associated with morphological changes during the laying cycle. We observed that C1 was highly developed, apoptosis rate was highest in C2, and C3 attained the smallest size. Through RNA-sequencing, we obtained SUPPORTING INFORMATION Additional supporting information may be found online in the Supporting Information section. How to cite this article: Lu L, Xu X, Du X, et al. Transcriptome analyses to reveal the dynamic change mechanism of pigeon magnum during one egg-laying cycle.

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Section: Introductionmentioning

confidence: 99%

Transcriptome analyses to reveal the dynamic change mechanism of pigeon magnum during one egg‐laying cycle

Liu

et al. 2020

Molecular Reproduction Devel

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“…Structural formation is controlled by precise interactions between mineral and organic precursors, which are secreted by specialized oviduct cell populations under gene regulation [13,28]. Calcium is the major component of eggshell and many calcium transport genes (such as calbindin, ATP2B1, 2 and TRPV6) have been identi ed to be involved in the supply of calcium for mineralization.…”

Section: Discussionmentioning

confidence: 99%

Uterine Transcriptome Analysis Reveals Mechanism Underlying the Ultrastructure Differences of Eggshell in Young and Aged Laying Hens

Feng

Zhang

et al. 2020

Preprint

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Background Lower eggshell quality in the late laying period leads to economic loss. It is a major threat to the quality and safety of egg products. Age-related variations in ultrastructure were thought to induce this deterioration. Eggshell mineralization is a highly complex process under precise regulation of genes and biological pathways in uterus of laying hens. Herein, we evaluated the physical, mechanical and ultrastructure properties of eggshell and conducted RNA sequencing to learn the transcriptomic differences in uterus between laying hens in the peak (young hens) and late phase (aged hens) of production. Results The declined breaking strength and fracture toughness of eggshell were observed in aged hen group compared to those in young hen group, accompanied with ultrastructure variations including the increased thickness of mammillary layer and the decreased incidence of early fusion. During the initial stage of eggshell formation, a total of 183 differentially expressed genes (DEGs; 125 upregulated and 58 downregulated) were identified in uterus of laying hens in the late phase in relative to those at peak production. The DEGs annotated to Gene Ontology terms related to antigen processing and presentation were downregulated in aged hens compared to young hens. The contents of proinflammatory cytokine IL-1β in uterus were higher in aged hens relative to those in young hens. Besides, the genes of some matrix proteins potentially involved in eggshell mineralization, such as ovalbumin, versican and glypican 3, were also differentially expressed between two groups. Conclusions Altered gene expression of matrix proteins along with the compromised immune function in uterus of laying hens in the late phase of production may conduce to age-related impairments of eggshell ultrastructure and mechanical properties. The current study enhances our understanding of the age-related deteriorations in eggshell ultrastructure and provides potential targets for improvement of eggshell quality in the late laying period.

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Section: Discussionmentioning

confidence: 99%

Uterine Transcriptome Analysis Reveals mRNA Expression Changes Associated with the Ultrastructure Differences of Eggshell in Young and Aged Laying Hens

Feng

Zhang

et al. 2020

Preprint

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Background: Lower eggshell quality in the late laying period leads to economic loss. It is a major threat to the quality and safety of egg products. Age-related variations in ultrastructure were thought to induce this deterioration. Eggshell formation is a highly complex process under precise regulation of genes and biological pathways in uterus of laying hens. Herein, we evaluated the physical, mechanical and ultrastructure properties of eggshell and conducted RNA sequencing to learn the transcriptomic differences in uterus between laying hens in the peak (young hens) and late phase (aged hens) of production.Results: The declined breaking strength and fracture toughness of eggshell were observed in aged hen group compared to those in young hen group, accompanied with ultrastructure variations including the increased thickness of mammillary layer and the decreased incidence of early fusion. During the initial stage of eggshell formation, a total of 183 differentially expressed genes (DEGs; 125 upregulated and 58 downregulated) were identified in uterus of laying hens in the late phase in relative to those at peak production. The DEGs annotated to Gene Ontology terms related to antigen processing and presentation were downregulated in aged hens compared to young hens. The contents of proinflammatory cytokine IL-1β in uterus were higher in aged hens relative to those in young hens. Besides, the genes of some matrix proteins potentially involved in eggshell mineralization, such as ovalbumin, versican and glypican 3, were also differentially expressed between two groups.Conclusions: Altered gene expression of matrix proteins along with the compromised immune function in uterus of laying hens in the late phase of production may conduce to age-related impairments of eggshell ultrastructure and mechanical properties. The current study enhances our understanding of the age-related deteriorations in eggshell ultrastructure and provides potential targets for improvement of eggshell quality in the late laying period.

show abstract

Transcriptome analysis reveals differentially expressed genes and pathways for oviduct development and defense in prelaying and laying hens

Cited by 15 publications

References 51 publications

Transcriptome analyses to reveal the dynamic change mechanism of pigeon magnum during one egg‐laying cycle

Transcriptome analyses to reveal the dynamic change mechanism of pigeon magnum during one egg‐laying cycle

Uterine Transcriptome Analysis Reveals Mechanism Underlying the Ultrastructure Differences of Eggshell in Young and Aged Laying Hens

Uterine Transcriptome Analysis Reveals mRNA Expression Changes Associated with the Ultrastructure Differences of Eggshell in Young and Aged Laying Hens

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