Transcriptome analysis of yellow passion fruit in response to cucumber mosaic virus infection

Abstract

The cultivation and production of passion fruit (Passiflora edulis) are severely affected by viral disease. Yet there have been few studies of the molecular response of passion fruit to virus attack. In the present study, RNA-based transcriptional profiling (RNA-seq) was used to identify the gene expression profiles in yellow passion fruit (Passiflora edulis f. flavicarpa) leaves following inoculation with cucumber mosaic virus (CMV). Six RNA-seq libraries were constructed comprising a total of 42.23 Gb clean data. 1,545 differentially expressed genes (DEGs) were obtained (701 upregulated and 884 downregulated). Gene annotation analyses revealed that genes associated with plant hormone signal transduction, transcription factors, protein ubiquitination, detoxification, phenylpropanoid biosynthesis, photosynthesis and chlorophyll metabolism were significantly affected by CMV infection. The represented genes activated by CMV infection corresponded to transcription factors WRKY family, NAC family, protein ubiquitination and peroxidase. Several DEGs encoding protein TIFY, pathogenesis-related proteins, and RNA-dependent RNA polymerases also were upregualted by CMV infection. Overall, the information obtained in this study enriched the resources available for research into the molecular-genetic mechanisms of the passion fruit/CMV interaction, and might provide a theoretical basis for the prevention and management of passion fruit viral disease in the field.

Transcriptome analysis reveals the molecular mechanisms of response to an emergent yellow-flower disease in green Chinese prickly ash (Zanthoxylum schinifolium)

“…Based on the KEGG pathway and GO enrichment of transcriptome data, Guo et al showed a complex regulatory network for PM resistance in pumpkin that may also involve hormone signal transduction pathways 20 . Chen et al reported that genes associated with plant hormone signal transduction, detoxification, phenylpropanoid biosynthesis, photosynthesis and chlorophyll metabolism were significantly affected by CMV infection in yellow passion fruit 21 . Transcriptome results showed that the moderate resistance of Pinus pinaster to Fusarium circinatum may be explained by the expression profiles pertaining to early recognition of the pathogen, the induction of pathogenesis-related proteins and the activation of complex phytohormone signaling pathways 22 .…”

The Role of Plant Transcription Factors in the Fight against Plant Viruses

“…Previously, this TF was reported to interact with the nucleocapsid protein of sonchus yellow net virus (SYNV) in N. benthamiana [63]. Through RNA-seq analysis, upregulation of 7 differentially expressed genes (DEGs), including the NAC family transcription factors, was identified in passion fruit (Passiflora edulis) during the infection of CMV [64]. NAC1 TF was discovered through genetic regulation analysis using retrotransposons and suggested to play a critical role in hormonal regulation during groundnut bud necrosis virus (GBNV) and tomato leaf curl New Delhi virus (ToLCNDV) infection [65].…”

Novel 4-Chromanone-Derived Compounds as Plant Immunity Inducers against CMV Disease in Passiflora spp. (Passion Fruit)

“…(passion fruit) is highly nutritious and possesses an exquisite sweet taste, making it widely cherished by the majority of consumers [ 1 ]. However, with the extensive cultivation of passion fruit in China, diseases have proliferated across all provinces due to the introduction, sale, and propagation of infected seedlings [ 2 ]. Among these diseases, cucumber mosaic virus (CMV) disease stands out as one of the primary viral infections affecting passion fruit plants [ 2 , 3 ].…”