Transcriptome analysis of Ulmus pumila ‘Jinye’ responses to different shading involved in chlorophyll metabolism

Zhang, Shuang; Zuo, Lihui; Zhang, Jun; Chen, Panfei; Wang, Jinmao; Yang, Minsheng

doi:10.1007/s11295-017-1139-7

Cited by 19 publications

(13 citation statements)

References 13 publications

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“…pumila ‘Jinye’ is more vulnerable to diseases and insect pests, and its leaves appear albino, withered, or even dead in high-temperature or high-light intensity environments (August). Zhang [4] found another interesting phenomenon in which the leaf color will gradually change from yellow to green in shaded conditions. Moreover, growth was not inhibited significantly under shaded conditions.…”

Section: Discussionmentioning

confidence: 99%

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The Reason for Growth Inhibition of Ulmus pumila ‘Jinye’: Lower Resistance and Abnormal Development of Chloroplasts Slow Down the Accumulation of Energy

Zuo

Zhang

Liu

et al. 2019

IJMS

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Ulmus pumila ‘Jinye’, the colorful leaf mutant of Ulmus pumila L., is widely used in landscaping. In common with most leaf color mutants, U. pumila ‘Jinye’ exhibits growth inhibition. In this study, U. pumila L. and U. pumila ‘Jinye’ were used to elucidate the reasons for growth inhibition at the physiological, cellular microstructural, and transcriptional levels. The results showed that the pigment (chlorophyll a, chlorophyll b, and carotenoids) content of U. pumila L. was higher than that of U. pumila ‘Jinye’, whereas U. pumila ‘Jinye’ had a higher proportion of carotenoids, which may be the cause of the yellow leaves. Examination of the cell microstructure and RNA sequencing analysis showed that the leaf color and growth inhibition were mainly due to the following reasons: first, there were differences in the structure of the thylakoid grana layer. U. pumila L. has a normal chloroplast structure and clear thylakoid grana slice layer structure, with ordered and compact thylakoids. However, U. pumila ‘Jinye’ exhibited the grana lamella stacking failures and fewer thylakoid grana slice layers. As the pigment carrier and the key location for photosynthesis, the close stacking of thylakoid grana could combine more chlorophyll and promote efficient electron transfer promoting the photosynthesis reaction. In addition, U. pumila ‘Jinye’ had a lower capacity for light energy absorption, transformation, and transportation, carbon dioxide (CO2) fixation, lipopolysaccharide biosynthesis, auxin synthesis, and protein transport. The genes related to respiration and starch consumption were higher than those of U. pumila L., which indicated less energy accumulation caused the growth inhibition of U. pumila ‘Jinye’. Finally, compared with U. pumila ‘Jinye’, the transcription of genes related to stress resistance all showed an upward trend in U. pumila L. That is to say, U. pumila L. had a greater ability to resist adversity, which could maintain the stability of the intracellular environment and maintain normal progress of physiological metabolism. However, U. pumila ‘Jinye’ was more susceptible to changes in the external environment, which affected normal physiological metabolism. This study provides evidence for the main cause of growth inhibition in U. pumila ‘Jinye’, information for future cultivation, and information on the mutation mechanism for the breeding of colored leaf trees.

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Section: Discussionmentioning

confidence: 99%

“…In addition, the foliage of U. pumila ‘Jinye’ contains many proteins and has good palatability, rendering it suitable for animal feed and feed processing. Moreover, its fruit and bark can be used in medicines [4,5,6].…”

Section: Introductionmentioning

confidence: 99%

The Reason for Growth Inhibition of Ulmus pumila ‘Jinye’: Lower Resistance and Abnormal Development of Chloroplasts Slow Down the Accumulation of Energy

Zuo

Zhang

Liu

et al. 2019

IJMS

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“…The porphyrin and chlorophyll metabolism undergoes a process from being catalyzed by l -glutamate and synthesis of chl precursors to the formation of chl a/b [ 78 ]. CAO is well accepted as chlorophyll a oxygenase, catalyzing chl a to chl b [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Gene Expression Patterns Potentially Associated with Premature Senescence in Nicotiana tabacum L.

Zhao

et al. 2018

Molecules

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Senescence affects the remobilization of nutrients and adaption of the plant to the environment. Combined stresses can result in premature senescence in plants which exist in the field. In this study, transcriptomic analysis was performed on mature leaves and leaves in three stages of premature senescence to understand the molecular mechanism. With progressive premature senescence, a declining chlorophyll (chl) content and an increasing malonaldehyde (MDA) content were observed, while plasmolysis and cell nucleus pyknosis occurred, mitochondria melted, thylakoid lamellae were dilated, starch grains in chloroplast decreased, and osmiophilic granules increased gradually. Moreover, in total 69 common differentially expressed genes (DEGs) in three stages of premature senescing leaves were found, which were significantly enriched in summarized Gene Ontology (GO) terms of membrane-bounded organelle, regulation of cellular component synthesis and metabolic and biosynthetic processes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis suggested that the plant hormone signal transduction pathway was significantly enriched. The common DEGs and four senescence-related pathways, including plant hormone signal transduction, porphyrin and chlorophyll metabolism, carotenoid biosynthesis, and regulation of autophagy were selected to be discussed further. This work aimed to provide potential genes signaling and modulating premature senescence as well as the possible dynamic network of gene expression patterns for further study.

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“…Two factors related to chlorophyll synthesis are present in porphyrin and chlorophyll metabolism pathways: the composite of chlorophyll precursors and the constitution of chlorophyll a/b. The synthesis of chlorophyll precursors from L-glutamic acid to protoporphyrin IX is catalyzed by a series of enzymes [37]. In the present study, six genes ( Supplementary Table S5) related to chlorophyll synthesis were among the DEGs (FC ≥ 2; FDR ≤ 0.01) in this experiment.…”

Section: Chlorophyll Synthesismentioning

confidence: 49%

Integrated Analysis of Transcriptomic and Metabolomic Data Reveals the Mechanism by Which LED Light Irradiation Extends the Postharvest Quality of Pak-choi (Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee)

Yan¹,

Zuo²,

Zhou

et al. 2020

Biomolecules

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Low-intensity (10 μmol m−2 s−1) white LED (light-emitting diode) light effectively delayed senescence and maintained the quality of postharvest pakchoi during storage at 20 °C. To investigate the mechanism of LED treatment in maintaining the quality of pakchoi, metabolite profiles reported previously were complemented by transcriptomic profiling to provide greater information. A total of 7761 differentially expressed genes (DEGs) were identified in response to the LED irradiation of pak-choi during postharvest storage. Several pathways were markedly induced by LED irradiation, with photosynthesis being the most notable. More specifically, porphyrin and chlorophyll metabolism and glucosinolate biosynthesis were significantly induced by LED irradiation, which is consistent with metabolomics reported previously. Additionally, chlorophyllide a, chlorophyll, as well as total glucosinolate content was positively induced by LED irradiation. Overall, LED irradiation delayed the senescence of postharvest pak-choi mainly by activating photosynthesis, inducting glucosinolate biosynthesis, and inhibiting the down-regulation of porphyrin and chlorophyll metabolism pathways. The present study provides new insights into the effect and the underlying mechanism of LED irradiation on delaying the senescence of pak-choi. LED irradiation represents a useful approach for extending the shelf life of pak-choi.

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Transcriptome analysis of Ulmus pumila ‘Jinye’ responses to different shading involved in chlorophyll metabolism

Cited by 19 publications

References 13 publications

The Reason for Growth Inhibition of Ulmus pumila ‘Jinye’: Lower Resistance and Abnormal Development of Chloroplasts Slow Down the Accumulation of Energy

The Reason for Growth Inhibition of Ulmus pumila ‘Jinye’: Lower Resistance and Abnormal Development of Chloroplasts Slow Down the Accumulation of Energy

Transcriptome Analysis of Gene Expression Patterns Potentially Associated with Premature Senescence in Nicotiana tabacum L.

Integrated Analysis of Transcriptomic and Metabolomic Data Reveals the Mechanism by Which LED Light Irradiation Extends the Postharvest Quality of Pak-choi (Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee)

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