Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model

Abstract

In a mouse model of Graves’ disease (GD), diosgenin has been shown to have a therapeutic effect on GD by alleviating goitre. However, research on the effect of diosgenin on autoimmune thyroiditis (AIT) is lacking. In this study, transcriptomics was used to comprehensively analyse the protective effect of diosgenin against AIT in rats and the possible mechanism. The results showed that in the diosgenin-intervention group, compared to the model group, the expression of serum triiodothyronine, thyroxine, free triiodothyronine, and free thyroxine was decreased and that of thyroid-stimulating hormone was increased; these changes were accompanied by the downregulation of thyroglobulin, TSH receptor antibody and thyroid peroxidase expression in serum. Furthermore, transcriptome detection, RT-qPCR and immunohistochemistry verification revealed that in thyroid tissue, the relative mRNA and protein expression of cyclic adenosine 3′,5′-monophosphate (cAMP), protein kinase A (PKA) and cAMP response element-binding protein (Creb) were increased and the mRNA expression of S100 calcium-binding protein A9 (S100A9) was decreased in the diosgenin groups. In summary, diosgenin alleviates the development of AIT, possibly via the activation of the cAMP/PKA/Creb pathway and downregulation of S100A9 gene expression.

Anticancer Activity of Methyl Protodioscin against Prostate Cancer by Modulation of Cholesterol-Associated MAPK Signaling Pathway <i>via</i> FOXO1 Induction

“…54) Dioscoreaceae, is well known for its unique medical and nutritional properties. The active components of Dioscoreaceae reportedly exhibited therapeutic effects on autoimmune thyroiditis in a rat model, 55) lung ischemia/reperfusion injury, 56) and breast cancer. 57) Based on the in vitro and in vivo profiles reported here, MPD may be an essential compound for the in vivo therapeutic effects of Dioscoreaceae extract in patients with prostate cancer.…”

Vitamin D levels and VDR rs2228570 genetic variant in Autoimmune Thyroiditis

“…Genetics is a key factor contributing to the development and progression of AIT (3). Research suggests that various immune-related genes play a role in the genetic predisposition to Autoimmune Thyroiditis (AIT) (4). The vital involvement of the vitamin D receptor (VDR) and its system is integral to the inflammatory response mechanism that leads to autoimmunity.…”

Diosgenin derivatives developed from Pd(ii) catalysed dehydrogenative coupling exert an effect on breast cancer cells by abrogating their growth and facilitating apoptosis via regulating the AKT1 pathway

“…3,6,7 Pharmacologically, it is reported to have activities against cancers, 8,9,10 diabetes, 11–13 atherosclerosis, 14 arthritis, 15 hepatis fibrosis, 16,17 and thyroiditis. 18,19 Its neuroprotective and cardioprotective abilities were found to be effective in treating Alzheimer's, Parkinson's and heart diseases. 20–23 Caprospinol, diosgenin-3-caproate, was reported to reduce Aβ 1–42 amyloid deposits and enhance memory dysfunction in rats affected with Alzheimer's disease.…”