Transcriptome analysis of common and diverged circulating miRNAs between arterial and venous during aging

Abstract

Circulating miRNAs have received extensive attention as non-invasive biomarkers for prediction and diagnosis of disease. However, most samples have been obtained from peripheral venous blood. To evaluate whether peripheral venous miRNAs represent circulating miRNAs from all blood vessels under a given condition, such as aging, we compared the miRNA profiles of venous and arterial plasma between young and aged rats by Illumina next-generation sequencing. The DEseq2 tool was used to obtain differentially-expressed miRNAs. We observed 105 aging-related deregulated miRNAs in vein and 62 in artery, which were highly associated with cell survival and inflammation, respectively. On the other hand, the young and aged groups exhibited a unique arterial-venous bias. There were 54 differentially-expressed miRNAs in the young group and 42 in the aged group; only 8 miRNAs were shared. Further transcriptional factors enrichment analysis found that the shared miRNAs could be partially upregulated by NF-κB and SIRT1. These transcriptional factors could be organ-specific and/or regulated in physiological and aging states as possible causal factors. This study suggested the potential application of circulating miRNAs, which reflect the systematic response to certain conditions, such as aging, and the importance of origin selection for candidate circulating miRNAs.

Common diseases alter the physiological age-related blood microRNA profile

“…Different aspects can be used to provide an even more comprehensive systemic picture of miRNAs and aging. First, the cell free part of the blood is also correlated to miRNA aging 44 , 45 . One important aspect are vesicles.…”

Identification of extremely GC-rich micro RNAs for RT-qPCR data normalization in human plasma

“…Their extraction can be biased across samples and studies resulting from different extraction chemistries ( Tan et al, 2015 ; Parker et al, 2021 ). The comparison of miRNA stability across different cohorts can be hampered by various physiological parameters impairing their abundance such as subject’s age ( Noren Hooten et al, 2013 ; Ameling et al, 2015 ; Fehlmann et al, 2020 ; Maffioletti et al, 2020 ; Wang et al, 2020 ), gender ( Duttagupta et al, 2011 ; Ameling et al, 2015 ), pregnancy ( Tay et al, 2017 ) or ethnicity ( Patel et al, 2019 ). Moreover, levels of circulating miRNAs can be impacted by alcohol consumption ( Ignacio et al, 2015 ), dietary habit ( Tarallo et al, 2014 ; Liu et al, 2020 ), poor sleep quality ( Baek et al, 2021 ), obesity parameters (body mass index ( Ameling et al, 2015 ), levels of visceral and subcutaneous adipose tissue, or body-fat percentage ( Munetsuna et al, 2018 )), settlement ( e.g .…”

Systems approaches to investigate the role of NF-κB signaling in aging

“…miRNA analysis has also been conducted to explore the influence of aging. Transcriptome analysis of miRNA from the peripheral blood of young and aged rats showed that miR-136-3p and miR-503-3p are differentially expressed with aging and are regulated by NF-κB and SIRT1 [ 95 ].…”