2016
DOI: 10.1073/pnas.1616627113
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Abstract: The promoter in HIV type 1 (HIV-1) proviral DNA contains three sequential guanosines at the U3-R boundary that have been proposed to function as sites for transcription initiation. Here we show that all three sites are used in cells infected with HIV-1 and that viral RNAs containing a single 5′ capped guanosine ( Cap 1G) are specifically selected for packaging in virions, consistent with a recent report [Masuda et al. (2015) Sci Rep 5:17680]. In addition, we now show that transcripts that begin with two or thr… Show more

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Cited by 69 publications
(110 citation statements)
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References 52 publications
(111 reference statements)
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“…The lack of base pair covariation has led some to question the validity of the tandem three-way junction structure [40], especially in view of strong evidence that residues of SD adopt a hairpin structure [8,40,41]. Although it is unfortunate that sequence conservation was too high for a conclusive phylogenetic assessment, it is certainly possible (likely, we believe) that the three-way junction and hairpin structures are both formed in infected cells, possibly a consequence of heterogeneous transcription start site usage (see below) [42]. …”
Section: Nmr Structure Of the Hiv-1 Rna Packaging Signalmentioning
confidence: 99%
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“…The lack of base pair covariation has led some to question the validity of the tandem three-way junction structure [40], especially in view of strong evidence that residues of SD adopt a hairpin structure [8,40,41]. Although it is unfortunate that sequence conservation was too high for a conclusive phylogenetic assessment, it is certainly possible (likely, we believe) that the three-way junction and hairpin structures are both formed in infected cells, possibly a consequence of heterogeneous transcription start site usage (see below) [42]. …”
Section: Nmr Structure Of the Hiv-1 Rna Packaging Signalmentioning
confidence: 99%
“…An additional layer of regulation may come from the sequence of the RNA itself, determined by the transcription start site (TSS) used during genome synthesis [42,62]. Shortly after initiation of RNA synthesis, the HIV-1 genome is co-transcriptionally capped by a 5′-5′ triphosphate linked 7-methylguanosine moiety (7MeG) [62,63,64,65,66].…”
Section: Transcriptional Start Site Heterogeneity Modulates Genomementioning
confidence: 99%
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