Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of japonica rice to chilling stress

Abstract

BackgroundThe transcriptional regulatory network involved in low temperature response leading to acclimation has been established in Arabidopsis. In japonica rice, which can only withstand transient exposure to milder cold stress (10°C), an oxidative-mediated network has been proposed to play a key role in configuring early responses and short-term defenses. The components, hierarchical organization and physiological consequences of this network were further dissected by a systems-level approach.ResultsRegulatory clusters responding directly to oxidative signals were prominent during the initial 6 to 12 hours at 10°C. Early events mirrored a typical oxidative response based on striking similarities of the transcriptome to disease, elicitor and wounding induced processes. Targets of oxidative-mediated mechanisms are likely regulated by several classes of bZIP factors acting on as1/ocs/TGA-like element enriched clusters, ERF factors acting on GCC-box/JAre-like element enriched clusters and R2R3-MYB factors acting on MYB2-like element enriched clusters.Temporal induction of several H2O2-induced bZIP, ERF and MYB genes coincided with the transient H2O2 spikes within the initial 6 to 12 hours. Oxidative-independent responses involve DREB/CBF, RAP2 and RAV1 factors acting on DRE/CRT/rav1-like enriched clusters and bZIP factors acting on ABRE-like enriched clusters. Oxidative-mediated clusters were activated earlier than ABA-mediated clusters.ConclusionGenome-wide, physiological and whole-plant level analyses established a holistic view of chilling stress response mechanism of japonica rice. Early response regulatory network triggered by oxidative signals is critical for prolonged survival under sub-optimal temperature. Integration of stress and developmental responses leads to modulated growth and vigor maintenance contributing to a delay of plastic injuries.

SALT-RESPONSIVE ERF1 Regulates Reactive Oxygen Species-Dependent Signaling during the Initial Response to Salt Stress in Rice

“…As H 2 O 2 levels increase rapidly upon salt stress (Hong et al, 2009), we propose that the induction of these TF genes is at first regulated by H 2 O 2 -dependent pathways rather than by signaling pathways mediated by ABA. Similarly, during chilling stress, it has been demonstrated that genes induced by oxidative stress are activated earlier than those responsive to ABA (Yun et al, 2010). It is notable that ABA and ROS signaling pathways affect each other through feed-forward and feedback loops.…”

“…Similarly, the early response regulatory network triggered by oxidative signals is of great importance for the adaptation of rice to cold stress (Cheng et al, 2007). Moreover, genes responding to oxidative stress are activated earlier than those mediated by ABA (Yun et al, 2010).…”

“…In addition, targets of MAPK proteins are mainly TFs (Popescu et al, 2009), underlining the direct connection with the MAPK signal cascade. The expression of many TF genes, including members of the HSF, WRKY, C 2 H 2 zinc-finger, bZIP, MYB, and AP2/ERF families, has been shown to be H 2 O 2 induced (Miao et al, 2004;Nishizawa et al, 2006;Davletova et al, 2005;Sun et al, 2010;Yun et al, 2010). Several zinc-finger proteins (ZFPs) contribute to salt tolerance by modulating oxidative stress responses.…”

Oxygen Metabolism in Chloroplast

“…The reversible redox modulation of Cys residues of the proteins is perhaps the most obvious mechanism for the H 2 O 2 -mediated activation of MAPK pathways. Transcriptomic analyses of Arabidopsis plants have revealed hundreds of H 2 O 2 -responsive genes (Ding et al, 2010;Yun et al, 2010). It was shown that H 2 O 2 produced inside the chloroplasts can leave the chloroplasts thus escaping the effective antioxidant systems located inside the chloroplast .…”

Respuesta transcripcional de Populus a bifenilos policlorados: Aspectos aplicados a la fitorremediación

“…Sin embargo también se ha encontrado su participación en mecanismos de respuesta a estrés abiótico (Alves et al, 2015;. Ejemplos: estrés por sequía (Davey et al, 2009;Golldack et al, 2011;Ranjan et al, 2012;Yan et al, 2012; ELEMENTOS REGULADORES Bhardwaj et al, 2013;Cao et al, 2013); estrés por temperatura, la cual incluye altas temperaturas, bajas temperaturas y congelación (Rensink et al, 2005;Lim et al, 2006;Bedon et al, 2010;Yun et al, 2010;Jiang et al, 2013;Tian et al, 2013); estrés por metales, como el hierro (Colangelo y Guerinot, 2004;Palmer et al, 2013) y el aluminio (Zeng et al, 2012;Yang et al, 2012b); y estrés por luz (Stracke et al, 2010;Rius et al, 2012), entre otros.…”