Transcriptional profiling of immune genes in bovine monocyte-derived macrophages exposed to bacterial antigens

Taraktsoglou, Maria; Szalabska, Urszula; Magee, David A.; Browne, John A.; Sweeney, T.; Gormley, Eamonn; MacHugh, David E.

doi:10.1016/j.vetimm.2010.12.002

Cited by 40 publications

(41 citation statements)

References 61 publications

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“…At the farm level, animals in similar physiological states under common environmental conditions also exhibit variable susceptibility to development of clinical mastitis. Various cell models have been used to investigate potential sources for these interanimal differences, including neutrophils (Sohn et al, 2007;Revelo and Waldron, 2012), monocyte-derived macrophages (Taraktsoglou et al, 2011), and mammary epithelial cells (Lahouassa et al, 2007;Brand et al, 2011). As model cells, neutrophils and macrophages are difficult to cryopreserve for future studies and can become activated during isolation and culturing processes, potentially adding to experimental variation.…”

Section: Introductionmentioning

confidence: 99%

Cow-to-cow variation in fibroblast response to a toll-like receptor 2/6 agonist and its relation to mastitis caused by intramammary challenge with Staphylococcus aureus

Benjamin

Green

Hayden

et al. 2015

Journal of Dairy Science

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Staphylococcus aureus is a common cause of chronic mammary gland infections in dairy cattle. However, the inflammatory response and duration of infection following pathogen exposure is variable between individual animals. To investigate interanimal differences in immune response, dermal fibroblast cultures were established from skin biopsies collected from 50 early lactation Holstein cows. The fibroblasts ability to produce IL-8 in response to a 24-h treatment with a synthetic toll-like receptor 2/6 agonist (Pam2CSK4) was used to assign a response phenotype to the animals. Five high-responding and 5 low-responding animals were then selected for an intramammary challenge with S. aureus to evaluate differences in the inflammatory response, chronicity of infection, and development of antibodies to the pathogen. All animals exhibited clinical symptoms of mastitis at 24h postchallenge. Animals previously classified as high responders experienced a greater inflammatory response characterized by elevated levels of milk somatic cell count, IL-8, and BSA following the challenge compared with low responders. In addition, antibodies toward the challenge strain of S. aureus reached higher levels in whey from the challenged gland of high responders compared with low responders. Despite the antibody response, all 5 high responders were chronically infected for the 6-wk duration of the study, whereas 2 of the low responders cleared the infection, although 1 of these did become reinfected. The observed differences between animals classified as low and high responders based on their fibroblast responsiveness suggests that this cell type can be used to further examine the causes of interanimal variation in response to mammary infection.

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Section: Introductionmentioning

confidence: 99%

Cow-to-cow variation in fibroblast response to a toll-like receptor 2/6 agonist and its relation to mastitis caused by intramammary challenge with Staphylococcus aureus

Benjamin

Green

Hayden

et al. 2015

Journal of Dairy Science

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“…MDMs are generally induced with lipopolysaccharide (LPS) and concanavalin A (ConA) to validate the expression and function of different IR genes (4)(5)(6)(7)(8). However, the degree of immune response to these antigens varies due to their structural differences and mode of action.…”

Section: Introductionmentioning

confidence: 99%

“…In domestic ruminants such studies are mostly reported in bovine (5,8,27). Sufficient data are lacking to make certain generic predictions and to fully ascertain if similar immunological paradigms apply in goats as in other ruminants.…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide and Concanavalin A Differentially Induce the Expression of Immune Response Genes in Caprine Monocyte Derived Macrophages

Walia

Kumar

Mitra

2015

Animal Biotechnology

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Monocyte derived macrophages (MDMs), as an in vitro model in pathogen challenge studies, are generally induced with lipopolysaccharide (LPS) and concanavalin A (ConA) to assay cellular immunity. General immune responses to LPS and ConA have been studied in a wide range of species, but similar studies are limited to goats. In the present study, caprine MDMs were induced with LPS and ConA and the expression profile of immune response (IR) genes, namely, Tumor Necrosis Factor Alpha (TNFA), Interferon Gamma (IFNG), Interleukin 2 (IL2), Granulocyte Macrophage Colony Stimulating Factor (GMCSF), Interleukin 10 (IL10), Transforming Growth Factor Beta (TGFB), Natural Resistance-Associated Macrophage Protein-1 (NRAMP1), inducible nitric oxide synthase (NOS2), and caspase1 (CASP1) were studied to compare the potential of LPS and ConA in initiating immune responses in goat macrophages. Real Time quantitative PCR (RT-qPCR) analysis revealed that both LPS and ConA caused an upregulation (p < 0.05) of GMCSF, TGFB1, IL10, and IFNG and down-regulation of NRAMP1. TNFA and IL2, and NOS2 were upregulated (p < 0.05) by ConA and LPS, respectively. Whereas, the expression of CASP1 remain unaltered. Comparatively, the effect of ConA was more pronounced (p < 0.05) in regulating the expression of IR genes suggesting its suitability for studying the general immune responses in caprine MDM.

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“…Functional traits are even more important in buffalo, as this species of livestock is susceptible to endemic diseases in many parts of the world, leading to important economic losses. Because the most dangerous zoonosis is brucellosis, and because infected animals must be killed according to the Italian law (Ministerial Decree N. 84/1991), attempts have been made to detect potentially disease-resistant animals (Capparelli et al, 2007) without infecting them (Taraktsoglou et al, 2011). …”

mentioning

confidence: 99%

“…Several authors have suggested using in-vitro systems to reproduce many of the intracellular events that occur during in-vivo bacterial exposure (Kovářová et al, 2003; Taraktsoglou et al, 2011). …”

mentioning

confidence: 99%

In-Vitro Indicators of Natural Resistance and Milk-Producing Ability in Dairy Buffaloes (Bubalus bubalis)

Miarelli

Signorelli

2014

Journal of Applied Animal Welfare Science

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The aim of this study was to explore the possibility of detecting novel phenotypes of natural resistance at the molecular level through the in-vitro stimulation of monocyte-derived macrophages (MDMs). This study was conducted with 16 healthy buffaloes who were reared for milk production and for whom data on milk-producing ability were available for several lactations. MDMs from circulating monocytes were activated with interferon-gamma and lipopolysaccharide. The response was evaluated using Western blotting to detect the presence of 2 types of proteins separated by electrophoresis: tyrosine-phosphorylated proteins, which are indicators of the dynamic control of biochemical pathways, and IkB-alpha (Kappa light polipeptide gene enhancer in B-cells Inhibitor, alpha) protein, which controls the activity of nuclear factor kappa-light-chain-enhancer of activated B cells—a transcription factor that is responsible for the expression of proinflammatory cytokines. The results showed that the buffaloes who were positive for IkB-alpha proteins had a significantly higher milk-producing ability than the buffaloes who did not express IkB-alpha. On the contrary, no significant difference was detected between the high and low milk-producing buffaloes with regard to the presence of tyrosine-phosphorylated proteins. This preliminary study indicated that it may be possible to identify the more disease-resistant nonhuman animals on a molecular level. The results, therefore, indicate that an intense selection toward the increase of milk yield could impair natural disease resistance in future dairy buffalo generations.

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Transcriptional profiling of immune genes in bovine monocyte-derived macrophages exposed to bacterial antigens

Cited by 40 publications

References 61 publications

Cow-to-cow variation in fibroblast response to a toll-like receptor 2/6 agonist and its relation to mastitis caused by intramammary challenge with Staphylococcus aureus

Cow-to-cow variation in fibroblast response to a toll-like receptor 2/6 agonist and its relation to mastitis caused by intramammary challenge with Staphylococcus aureus

Lipopolysaccharide and Concanavalin A Differentially Induce the Expression of Immune Response Genes in Caprine Monocyte Derived Macrophages

In-Vitro Indicators of Natural Resistance and Milk-Producing Ability in Dairy Buffaloes (Bubalus bubalis)

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