Transcriptional activation of OCT4 by the ETS transcription factor PEA3 in NCCIT human embryonic carcinoma cells

Abstract

Edited by Ned ManteiKeywords: ETS family transcription factor Polyomavirus enhancer activator 3 OCT4 promoter Embryonic carcinoma NCCIT a b s t r a c tWe examined the molecular mechanism of OCT4 gene regulation by polyomavirus enhancer activator 3 (PEA3) in NCCIT cells. Endogenous PEA3 and OCT4 were significantly elevated in undifferentiated cells and reduced upon differentiation. PEA3 knockdown led to a reduction in OCT4 levels. OCT4 promoter activity was significantly up-regulated by dose-dependent PEA3 overexpression. Deletion and site-directed mutagenesis of the OCT4 promoter revealed a putative binding site within the conserved region 2 (CR2). PEA3 interacted with the binding element within CR2 in NCCIT cells. This study reveals the molecular details of the mechanism by which the oncogenic factor PEA3 regulates OCT4 gene expression as a transcriptional activator.

Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

“…Oct4 was also demonstrated to promote cell cycle progression in esophageal carcinoma (8), promote colony formation of glioma cells (9), and increase transmigration capacity in melanoma cells (10). These previous studies suggest that deregulation and dysfunction of Oct4 contribute to malignant establishment of a cancer stem cell phenotype (5).…”

“…For example, the expression of Oct4 was indicated to be significantly correlated with early hepatocellular carcinoma recurrence (4). Oct4 has been identified as overexpressed in human embryonic carcinoma (5), prostate cancer (6), and bladder cancer cells, and been associated with enhanced migration and invasion (7). Oct4 was also demonstrated to promote cell cycle progression in esophageal carcinoma (8), promote colony formation of glioma cells (9), and increase transmigration capacity in melanoma cells (10).…”

Growth and Differentiation Factor 3 Is Transcriptionally Regulated by OCT4 in Human Embryonic Carcinoma Cells

“…38) We also previously reported that OCT4 and GDF3 are highly expressed in pluripotent human EC NCCIT cells and that this expression is reduced upon RA treatment in a time-dependent manner. 30,36,37,39,40) However, there is no report concerning the interaction between OCT4 and GDF3 at the transcriptional level. Therefore, to investigate their transcriptional relationship in pluripotent germ cell-derived EC cells, we first examined the expression of GDF3 and OCT4 in NCCIT cells during RA-mediated differentiation.…”

“…34,35) Western Blot Analysis NCCIT cells were treated with RA for different amounts of time (0, 2, 4, 6, 8, 10 d) and harvested, or transfected with OCT4-targeting shRNA or the Flag-tagged OCT4 expression vector using the ViaFect transfection reagent (Promega) and harvested 48 h later. Western blotting was performed as previously described 36,37) with a minor modification. Membranes were blocked in 1% bovine serum albumin and incubated with anti-OCT4 polyclonal Each primary antibody-complexed membrane was further incubated with a horseradish peroxidase-conjugated secondary antibody (1 : 5000, Santa Cruz Biotechnology).…”

ETV transcriptional upregulation is more reliable than RNA sequencing algorithms and FISH in diagnosing round cell sarcomas with CIC gene rearrangements

“…Expression levels of CIC, ETV1, ETV4, ETV5, and WT1 were evaluated on both RNAseq platforms in comparison to other soft tissue tumors in each platform. The mRNA expression of known PEA3 family downstream target genes, including SPRY2, SPRY4, SPRED2, GPR20, DUSP6 GBX2, FGF8, POU5F1, MMP1/2/3/7/9, TWIST1, SNAI1/2, PTGS2, BAX, CCND2, ZEB1, PTK2, PLAU, ICAM1, VEGFA, NOTCH1, NOTCH4, and SPP1 was investigated from the whole transcriptome sequencing platform, while FRG1, PITX1, ZSCAN4, RFPL2, TRIM43, and LEUTX, were evaluated as DUX4 targets …”