Transcriptional Activation of Nuclear-Related Factor 2 by FK506 in Jurkat T Cells

Jeong, Young-Ran; Chung, Moon‐Soo; Chung, Young-Taek; Choi, Seong-Ho; Choi, Soo Jin Na; Chung, Sang Young

doi:10.1016/j.transproceed.2014.10.056

Cited by 3 publications

(3 citation statements)

References 23 publications

(1 reference statement)

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“…18 Of these, Nrf-2 might play a critical role in the upregulation of HO-1 gene expression. 18,19,24,25 In physiological state, Nrf-2 exists in the cytoplasm because Kelch-like epichlorohydrin-associated protein-1 prevents its nuclear translocation; activated MAPKs make Nrf-2 dissociate from Kelch-like epichlorohydrin-associated protein-1 and translocate to the nucleus. 18,19,24,25 The precise link between MAPK activity and Nrf-2 activation in response to TENS is at present not clear and needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…Activated MAPKs convert extracellular stimuli into intracellular responses by activating downstream transcriptional factors, such as the nuclear factor erythroid-derived 2 like 2 (Nrf-2), activator protein-1, and nuclear factor-kappa B, resulting in the de novo synthesis of mRNA 18 . Of these, Nrf-2 might play a critical role in the upregulation of HO-1 gene expression 18,19,24,25 . In physiological state, Nrf-2 exists in the cytoplasm because Kelch-like epichlorohydrin-associated protein-1 prevents its nuclear translocation; activated MAPKs make Nrf-2 dissociate from Kelch-like epichlorohydrin-associated protein-1 and translocate to the nucleus 18,19,24,25 .…”

Section: Discussionmentioning

confidence: 99%

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Effects of Preconditioning With Transcutaneous Electrical Nerve Stimulation Monitored by Infrared Thermography on the Survival of Pedicled Perforator Flaps in a Rat Model

2022

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Objective: Pedicled perforator partial or complete necrosis with a rate of 13.7%. This study was undertaken to test whether preconditioning with transcutaneous electrical nerve stimulation (TENS) monitored by infrared thermography protect against partial necrosis by converting the choke anastomoses to the true anastomoses via inducing heme oxygenase-1 (HO-1) in a rat pedicled perforator flap model. Methods: Seventy-two Sprague-Dawley rats were randomly assigned to the control, the TENS, the TENS + SnPP (tin protoporphyrin; HO-1 activity inhibitor; 50 μmol/kg) and the TENS +0.9% saline groups. On the unilateral dorsum of the rats, a rectangular flap donor site of 11 Â 3 cm was marked out, which contained three perforator angiosomes and two choke zones. On days 1, 3 and 4, 1 hour of TENS (biphasic pulses, 25 mA, 80 Hz, 200 μs) was applied to the flap donor sites, respectively. On day 5, after the flap donor sites were assessed by infrared thermography, the flaps were harvested based on the deep circumflex iliac artery perforator. Results: Infrared thermography showed that the choke zones in the flap donor sites presented white in the TENS and the TENS +0.9% saline groups, whereas they presented red in the control and the TENS + SnPP groups. Postmortem arteriography showed that the number of arterioles across each choke zone significantly increased in the TENS and the TENS +0.9% saline groups compared with the control and the TENS + SnPP groups. Immunohistochemistry and western blot showed a significant increase in HO-1 in the choke zones after TENS preconditioning. The necrotic area percentage of the flaps was significantly decreased in the TENS (4.3% ± 2.6%) and the TENS +0.9% saline groups (4.5% ± 2.3%) compared with the control (24.8% ± 5.0%) ( P < 0.001); there was no significant difference between the TENS and the TENS + SnPP (24.4% ± 7.3%) groups. Conclusions: These data show that TENS preconditioning monitored by infrared thermography might be a promising strategy to prevent pedicled perforator flaps from partial necrosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Preconditioning With Transcutaneous Electrical Nerve Stimulation Monitored by Infrared Thermography on the Survival of Pedicled Perforator Flaps in a Rat Model

2022

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“…Tacrolimus binds to the FK506-binding protein (FKBP) and it inhibits calcineurin (CaN), a calcium and calmodulin-dependent serine threonine protein phosphatase, resulting in the inhibition of NFAT signaling cascades and thus suppression of the T-lymphocytes mediated signal transduction pathway leading to transcription of interleukin 2 (IL2) [16]. The immunomodulatory effects of FK506 on MAPK, NF-kB, and NRF2 pathway has been reported previously in neurodegenerative disorders [3,4,17].…”

Section: Introductionmentioning

confidence: 99%

Combinatory FK506 and Minocycline Treatment Alleviates Prion-Induced Neurodegenerative Events via Caspase-Mediated MAPK-NRF2 Pathway

Shah

Zhao

Taglialatela

et al. 2019

IJMS

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Transcription factors play a significant role during the symptomatic onset and progression of prion diseases. We previously showed the immunomodulatory and nuclear factor of activated T cells’ (NFAT) suppressive effects of an immunosuppressant, FK506, in the symptomatic stage and an antibiotic, minocycline, in the pre-symptomatic stage of prion infection in hamsters. Here we used for the first time, a combinatory FK506+minocycline treatment to test its transcriptional modulating effects in the symptomatic stage of prion infection. Our results indicate that prolonged treatment with FK506+minocycline was effective in alleviating astrogliosis and neuronal death triggered by misfolded prions. Specifically, the combinatory therapy with FK506+minocycline lowered the expression of the astrocytes activation marker GFAP and of the microglial activation marker IBA-1, subsequently reducing the level of pro-inflammatory cytokines interleukin 1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α), and increasing the levels of anti-inflammatory cytokines IL-10 and IL-27. We further found that FK506+minocycline treatment inhibited mitogen-activated protein kinase (MAPK) p38 phosphorylation, NF-kB nuclear translocation, caspase expression, and enhanced phosphorylated cAMP response element-binding protein (pCREB) and phosphorylated Bcl2-associated death promoter (pBAD) levels to reduce cognitive impairment and apoptosis. Interestingly, FK506+minocycline reduced mitochondrial fragmentation and promoted nuclear factor–erythroid2-related factor-2 (NRF2)-heme oxygenase 1 (HO-1) pathway to enhance survival. Taken together, our results show that a therapeutic cocktail of FK506+minocycline is an attractive candidate for prolonged use in prion diseases and we encourage its further clinical development as a possible treatment for this disease.

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Protective effects of selenium in tacrolimus-induced lung toxicity: potential role of heme oxygenase 1

Ibrahim

El-Tahway

Abdalla

et al. 2021

Can. J. Physiol. Pharmacol.

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Abstract The present study aimed to evaluate the protective effects of selenium administration against tacrolimus (Tac) induced lung toxicity and to assess the relation between heme oxygenase 1 (HO-1) and these effects. The study was conducted on 36 Wistar male albino rats equally divided into 4 groups: (1) normal control, (2) Selenium (Sel 0.1mg/kg/day p.o for four weeks), (3) TAC 3mg/ml as single oral dose on 27th day (4) Tac + Sel. Lung tissues, lung homogenate, and bronchoalveolar lavage of the sacrificed animals were investigated biochemically, histopathologically, by immunohistochemistry or by PCR. Tac group showed significantly lower expression of HO-1. Administration of selenium was associated with increased HO-1 expression. Oxidative (malondialdehyde: MDA, reduced glutathione: GSH, superoxide dismutase: SOD, myeloperoxidase: MPO, glutathione peroxidase activity: GPx) and nitrosative stress (Nitric oxide: NO) markers and markers of inflammation (Interleukins: IL1β, 6 and 10) showed changes corresponding to HO-1 levels in rats groups. Tac group showed the highest expression of caspase-3. Selenium exerted protective role against tacrolimus induced lung toxicity.

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Transcriptional Activation of Nuclear-Related Factor 2 by FK506 in Jurkat T Cells

Cited by 3 publications

References 23 publications

Effects of Preconditioning With Transcutaneous Electrical Nerve Stimulation Monitored by Infrared Thermography on the Survival of Pedicled Perforator Flaps in a Rat Model

Effects of Preconditioning With Transcutaneous Electrical Nerve Stimulation Monitored by Infrared Thermography on the Survival of Pedicled Perforator Flaps in a Rat Model

Combinatory FK506 and Minocycline Treatment Alleviates Prion-Induced Neurodegenerative Events via Caspase-Mediated MAPK-NRF2 Pathway

Protective effects of selenium in tacrolimus-induced lung toxicity: potential role of heme oxygenase 1

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