Transcription ofc-mos protooncogene in the pig involves both tissue-specific promoters and alternative polyadenylation sites

Involvement of polyadenylation status on maternal gene expression during in vitro maturation of porcine oocytes

“…Interestingly, three major bands were observed for C-mos PAT by gel electrophoresis, suggesting that at least three 3 0 -UTR variants exist in pig oocyte. This was confirmed in a previous study in which the C-mos gene was observed to have three transcript variants with different 3 0 -UTR lengths (Newman and Dai, 1996). Further, C-mos was shown to be stored in a polyadenylated form in mature pig oocyte (Dai et al, 2005).…”

“…Our data suggested that C-mos and BMP15 share the same mRNA expression patterns during oocyte maturation. Previously, expression of C-mos was identified by RNase protection assay in growing pig oocytes, and remained stable during oocyte maturation (Newman and Dai, 1996). The findings reported here regarding the significant up-regulation observed at 28 hr may be a result of the more sensitive real-time PCR assay used in this work.…”

Mos 3′ UTR regulatory differences underlie species‐specific temporal patterns of Mos mRNA cytoplasmic polyadenylation and translational recruitment during oocyte maturation

“…The murine Mos 3′ UTR has two functional CPE sequences, either of which can direct maturation-dependent cytoplasmic polyadenylation (Gebauer et al, 1994). Similarly, rat and pig Mos 3′ UTR sequences have been reported (van der Hoorn and Firzlaff, 1984; Newman and Dai, 1996) and are predicted to contain two functional U 5 A CPEs. While mutational disruption of the first CPE sequence (U 5 A) completely ablated maturation-dependent polyadenylation of the human Mos mRNA (Fig.…”

Isorhamnetin improves in vitro maturation of oxidative stress-exposed porcine oocytes and subsequent embryo development

“…MAPK is activated at the germinal vesicle breakdown stage, localizes to the cytoplasm and around chromosomes from MI to MII, and is essential for resuming meiosis in MII and maintaining arrest (Villa-Diaz and Miyano, 2004). MAPK is activated by MOS protein, an active component of cytostatic factor, which is responsible for meiotic arrest at MII (Newman and Dai, 1996). GDF9 and BMP15 play an important role in the regulation of fertility (Juengel et al, 2004) and regulate oogenesis by interacting with each other (Hussein et al, 2006).…”