Transcription of biliary glycoprotein I gene in malignant and non‐malignant human liver tissues

Hinoda, Yuji; Imai, Kohzoh; Nakagawa, Naoaki; Ibayashi, Yukihiro; Nakano, Tatsumi; Paxton, Raymond J.; Shively, John E.; Yachi, Akira

doi:10.1002/ijc.2910450516

Cited by 26 publications

(18 citation statements)

References 15 publications

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“…CGM1, CGM6, BGP and possibly CGM7 [23][24][25]. BGP is also present in epithelial cells of bile canaliculi [26], It is also found in hepatocellu lar and other carcinomas [27], but is often down-regulated in tumours compared to the corresponding normal tissues [28]. Trans cripts for CGM2 have so far been found only in normal colonic mucosa, colonic and muci nous ovarian carcinomas, but not in leuko cytes (results to be published).…”

Section: Expression Of Cea Family Membersmentioning

confidence: 99%

Molecular Cloning and Expression of Carcinoembryonic Antigen Gene Family Members

Thompson

1995

Tumor Biol

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The carcinoembryonic antigen (CEA) gene family consists of approximately 30 genes, of which 17 are transcriptionally active. These genes are tightly clustered on the long arm of chromosome 19. Two main subgroups are distinguishable, one encoding CEA and its classical non-specific cross-reacting antigens (NCAs) and the second encoding the pregnancy-specific glycoproteins (PSGs). The CEA family belongs to the immunoglobulin superfamily. CEA subgroup members are either membrane bound through a glycosyl phosphatidylinositol anchor, or are integral membrane proteins, whereas PSG molecules are secreted from the cell. Despite high sequence similarity, CEA family members reveal variable expression patterns, indicating different functions. Apart from its high expression in various adenocarcinomas, CEA is found in epi-thelia lining the gastrointestinal tract, in eccrine sweat glands, lung epithelia and testes. The NCAs are often coexpressed with CEA, but are also found in granulocytes. PSGs are coexpressed in syncytiotrophoblast cells of the placenta, subman-dibular salivary glands and fetal liver, as well as in choriocarcinomas and breast tumours.

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Section: Expression Of Cea Family Membersmentioning

confidence: 99%

Molecular Cloning and Expression of Carcinoembryonic Antigen Gene Family Members

Thompson

1995

Tumor Biol

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“…Human CEACAM1 plays a central role in mediating apoptosis during lumen formation in a three-dimensional model of mammary morphogenesis (18,19) and participates in angiogenesis in endothelial cells (5). In cancer, CEACAM1 has been shown to be down-regulated in liver (20,21), prostate (22)(23)(24), colon (25), lung (26), and breast carcinoma (27,28). The down-regulation in colon cancer occurs at the early adenoma stage (29) and in Ͼ90% of colon cancers (25).…”

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confidence: 99%

CEACAM1, a Cell-Cell Adhesion Molecule, Directly Associates with Annexin II in a Three-dimensional Model of Mammary Morphogenesis

Kirshner

Schumann

Shively

2003

Journal of Biological Chemistry

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The epithelial cell adhesion molecule CEACAM1 (carcinoembryonic antigen cell adhesion molecule-1) is down-regulated in colon, prostate, breast, and liver cancer. Here we show that CEACAM1-4S, a splice form with four Ig-like ectodomains and a short cytoplasmic domain (14 amino acids), directly associates with annexin II, a lipid raft-associated molecule, which is also downregulated in many cancers. Annexin II was identified using a glutathione S-transferase pull-down assay in which the cytoplasmic domain of CEACAM-4S was fused to glutathione S-transferase, the fusion protein was incubated with cell lysates, and isolated proteins were sequenced by mass spectrometry. The interaction was confirmed first by reciprocal immunoprecipitations using anti-CEACAM1 and anti-annexin II antibodies and second by confocal laser microscopy showing co-localization of CEACAM1 with annexin II in mammary epithelial cells grown in Matrigel. In addition, CEACAM1 co-localized with p11, a component of the tetrameric AIIt complex at the plasma membrane, and with annexin II in secretory vesicles. Immobilized, oriented peptides from the cytoplasmic domain of CEACAM1-4S were shown to directly associate with bovine AIIt, which is 98% homologous to human AIIt, with average K D values of about 30 nM using surface plasmon resonance, demonstrating direct binding of functionally relevant AIIt to the cytoplasmic domain of CEACAM1-4S.

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“…BGP is now known to be specifically expressed in cells of epithelial and myeloid origins, in colon [3], liver [4], tumor tissues or cell lines (colon, pancreas, liver, bladder and acute myelogeneous leukemia) [5 -71 and in granulocytes and leu-liver-specific factor A1 .…”

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confidence: 99%

Transcriptional control of the human biliary glycoprotein gene, a CEA gene family member down‐regulated in colorectal carcinomas

Hauck

Nédellec

Turbide

et al. 1994

European Journal of Biochemistry

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Biliary glycoprotein (BGP) isoantigens are derived by alternative splicing from a single gene and are the human homologs of rat C-CAM and the mouse Bgp species. These glycoproteins represent a family of cell-adhesion molecules. The mouse Bgp isoforms also act as receptors for the hepatitis viral capsid-protein. BGP is a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin supergene family, yet it displays restricted expression patterns and unique functions. Since the loss or reduced expression of BGP is associated with human colorectal carcinomas, the elements in its upstream regulatory region were analyzed. A cluster of transcriptional initiation sites and the minimal promoter, located within 150 bp upstream of the major transcriptional start site, were active in human colon carcinoma and hepatoma cells. Unlike the CEA gene, BGP gene transcription was not modulated by a silencer region; repetitive elements in the BGP upstream region were not involved in activation or repression. Footprinting experiments identified two cis-acting elements and mobility-shift assays demonstrated that these elements bound several transcription factors, among them, USF, HNF-4 and an AP-2-like factor. In cotransfection experiments, both the USF and HNF-4 transcription factors transactivate the RGP gene promoter and compete for the same regulatory element. The Spl transcription factor, shown to be involved in CEA gene transcriptional regulation, does not bind to the BGP gene promoter. We, therefore, propose that the relative distributions and interactions of these transcription factors mediate distinct transcriptional regulation of the BGP gene in colon and liver; this regulation could be distorted during the oncogenic process.

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Transcription of biliary glycoprotein I gene in malignant and non‐malignant human liver tissues

Cited by 26 publications

References 15 publications

Molecular Cloning and Expression of Carcinoembryonic Antigen Gene Family Members

Molecular Cloning and Expression of Carcinoembryonic Antigen Gene Family Members

CEACAM1, a Cell-Cell Adhesion Molecule, Directly Associates with Annexin II in a Three-dimensional Model of Mammary Morphogenesis

Transcriptional control of the human biliary glycoprotein gene, a CEA gene family member down‐regulated in colorectal carcinomas

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