1992
DOI: 10.1128/jvi.66.7.4228-4232.1992
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Abstract: In this report, we describe a mechanism by which human immunodeficiency virus type 1 (HIV-1)-infected cells can influence neighboring HIV-1-infected T lymphocytes and uninfected T cells as well. We have examined the interaction of T-cell and macrophage cell lines that are transfected with HIV-1 DNA by using cocultured lymphocytes. The HIV-1 constructs we used lack a functional pol gene and therefore do not produce infectious virus. Cocultivation results in the transcellular activation of the HIV long terminal … Show more

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Cited by 39 publications
(8 citation statements)
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References 23 publications
(19 reference statements)
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“…Our previous findings that Vpr inhibits cell growth and can activate cellular differentiation are also indications that Vpr may affect transcriptional events either directly or indirectly. The Vpr activity reported here might explain transcellular activation of the HIV long terminal repeat observed after coculture of HIV-infected cells with target cells containing reporter constructs (38). The experiments reported here do not indicate whether Vpr assists early or late HIV replication events in newly infected cells.…”
Section: Discussionmentioning
confidence: 57%
“…Our previous findings that Vpr inhibits cell growth and can activate cellular differentiation are also indications that Vpr may affect transcriptional events either directly or indirectly. The Vpr activity reported here might explain transcellular activation of the HIV long terminal repeat observed after coculture of HIV-infected cells with target cells containing reporter constructs (38). The experiments reported here do not indicate whether Vpr assists early or late HIV replication events in newly infected cells.…”
Section: Discussionmentioning
confidence: 57%
“…It was important to check the biological activity of secreted Tat in our experimental system. To this end, we first used a well‐established transcellular transactivation assay (Helland et al , 1991; Marcuzzi et al , 1992; Ensoli et al , 1993). Donor cells were transfected with Tat (WT or W11Y) or EGFP as a control, and recipient cells with luciferase reporter plasmids.…”
Section: Resultsmentioning
confidence: 99%
“…In addition to its role as a nuclear transactivator, Tat protein can be secreted and taken up by the neighboring uninfected cells [27][28][29][30][31][32]. Earlier studies suggested that Tat may function as a growth factor in the proliferation of Kaposi sarcomaderived cell lines [33].…”
Section: Role Of Tat and Cytokine Dysregulation By Hiv In Cnsmentioning
confidence: 99%