Trans-species polymorphism of class II Mhc loci in danio fishes

Graser, Robert T.; Ó'hUigín, Colm; Vincek, Vladimir; Meyer, Axel; Klein, Jan

doi:10.1007/s002510050087

Cited by 26 publications

(33 citation statements)

References 15 publications

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“…High polymorphism is one of the most characteristic features of the MHC genes; allelic MHC molecules bind and present specificity peptides, and the response of an organism toward certain pathogens can be influenced by MHC alleles present in different haplotypes. Allelic polymorphisms of MHC genes have been investigated in many species (Graser et al, 1996;Axtner and Sommer, 2007). For example, 76 alleles in 60 flounder individuals (Xu et al, 2008), 26 alleles in 36 Clethrionomys glareolus individuals (Axtner and Sommer, 2007), 27 alleles in 146 Apodemus flavicollis individuals (Meyer-Lucht and Sommer, 2005), and 34 alleles in 40 Gerbillurus paeba individuals (Harf and Sommer, 2005) were found.…”

Section: Discussionmentioning

confidence: 99%

“…The MHC class II molecule, consisting of one alpha chain and one beta chain, presents foreign peptides derived from extracellular pathogens to helper CD4 + T cells. Surprisingly, unlike mammals and other vertebrates, the class I and class II genes were found to reside on different linkage groups in teleosts (Graser et al, 1996). Thus, some scientists believe that the major histocompatibility (MH) genes are more appropriate designation in teleosts (Stet et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Allelic polymorphism, gene duplication and balancing selection of the MHC class II DAB gene of Cynoglossus semilaevis (Cynoglossidae)

Tj¹,

Yn²,

Rx³

2011

Genet. Mol. Res.

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ABSTRACT. Major histocompatibility complex (MHC) genes play an important role in the immune response of vertebrates. Allelic polymorphism and evolutionary mechanism of MHC genes have been investigated in many mammals, but much less is known in teleosts. We examined the polymorphism, gene duplication and balancing selection of the MHC class II DAB gene of the half-smooth tongue sole (Cynoglossus semilaevis); 23 alleles were found in this species. Gene duplication manifested as three to six distinct sequences at each domain in the same individuals. Non-synonymous substitutions occurred at a significantly higher frequency than synonymous substitutions in the PBR domain, suggesting balancing selection for maintaining polymorphisms at the MHC II DAB locus. Many positive selection sites were found to act very intensely on antigen-binding sites of MHC class II DAB gene.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Allelic polymorphism, gene duplication and balancing selection of the MHC class II DAB gene of Cynoglossus semilaevis (Cynoglossidae)

Tj¹,

Yn²,

Rx³

2011

Genet. Mol. Res.

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“…Ever since the first isolated MHC genes were studied in carp (Hashimoto et al, 1990), researchers have found and analyzed MHC genes in many teleosts, such as Atlantic salmon (Grimholt et al, 2003), zebrafish (Ono et al, 1992), half-smooth tongue sole (Li et al, 2010), and miiuy croaker (Xu et al, 2011). Studies have focused on their genome structure (Sato et al, 2000;Roney et al, 2004), polymorphism (Graser et al, 1996;Du et al, 2011), evolution (Hughes and Nei, 1989;Eizaguirre et al, 2012), mate choice (Landry et al, 2001), and other aspects. Expression studies showed that MHC class II genes are ubiquitously expressed, with high expression in immune tissues, such as spleen, kidney, gill, and intestine Li et al, 2010;Xu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of major histocompatibility complex class II genes in the stone flounder Kareius bicoloratus (Pleuronectidae)

Jiang

Li²,

Zhang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Major histocompatibility complex (MHC) class II genes play important recognition roles in the immune system in vertebrates. We cloned the MHC class II genes A and B in the stone flounder (Kareius bicoloratus). The full-length cDNA and DNA sequences of both genes were obtained, and their characteristic motifs were analyzed. The DNA sequence of stone flounder MHC class II A consists of four exons, while gene B contains six exons. The extra intron in gene B might be a common feature in most of its Acanthopterygii orthologs. Several conserved motifs were identified by multiple deduced amino acid sequence alignments of the two genes and their orthologs. The peptide sequences of α chain and β chain shared identity of 86.0-30.1% and 69.8-31.3% with their orthologs, respectively. Bayes phylogenetic trees showed that the stone flounder is closely related to the spotted halibut (Verasper variegates), and the half-smooth tongue sole (Cynoglossus semilaevis). Real-time quantitative PCR showed that in the stone flounder, both genes A and B are highly or moderately MHC class II genes cloning in stone flounder expressed in several tissues, including the intestine, spleen and gills, and less expressed or undetectable in the liver, kidney, brain, heart, and gonads. These expression patterns differed slightly from those in other teleosts. This might be a unique phenomenon in the stone flounder. This first study of MHC genes in stone flounder could provide reference data for comparative studies.

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“…The Giant Danio is a tropical fish belonging to the family Cyprinidae and phylogenetically closely related to Danio rerio [14] and is one of the big sized forms among Danionins. Giant Danio has been used as a model in retinal cone electrophysiology [15], fine structure of retinal epithelium circuitry and cell electrical coupling [16][17][18], trans species polymorphism in MHC complex [19], neurotrophic factor [20], body flow dynamics in swimming [21], deafferentation and olfactory bulb morphology [22], for vision experiment [23]. More recently, the Giant Danio has been proposed as a model to study skeletal muscle growth [24,25], cardiac remodeling and regeneration [26] visual impairments of retinal layers [27].…”

Section: Introductionmentioning

confidence: 99%

The Effects Induced Hyperglycemia on Adrenal Cortex Function in the Giant Danio Devario Aequipinnatus Embryos

Perumal¹

2017

JDMDC

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Purpose: The purpose of this study was to induce hyperglycemic experiment in Giant Danio embryos in relation to effects of hyperglycemia on adrenal cortex function and steroidogenesis. Methods:The wild specimens of Devario aequipinnatus fishes collected from the Cauvery river at Stanley Reservoir were induced for breeding without the use of any hormones and the matured embryos were obtained. The embryos of Giant Danio were induced for hyperglycemic stress, by immersing them in 0.0%, 0.3%, 0.6%, 0.9%, 1.2%, 1.5%, and 1.8% glucose solution, at 24 h, 48 h and 72 h. Result:The Giant Danio exposed to glucose solution appear to experience acute stress as indicated by the significant elevation in serum glucose and serum cortisol. The ranges of average cortisol level for all embryo samples exposed for 24 h, 48 h and 72 h in stock aquaria were: 1.2-6.4 µg/dL, 0.8 to 7.6 µg/dL and 0.8-9.6µg/dL respectively. Conclusion:The results showed positive correlation between the %glucose solution and the cortisol level, as well as the incubation time and the cortisol level as reported previously in other model systems. Keywords: The Effects Induced Hyperglycemia on Adrenal Cortex Function in the Giant Danio Devario Aequipinnatus Embryos 2/6Copyright: ©2017 Raja et al.gender, blood pressure, lifestyle, stress, perception of stress, and psychiatric illnesses are all known to affect cortisol secretion and metabolism as well as the increased risk of developing DM [28,29]. Using an animal model of DM would allow for control or elimination of many if not all of these confounding factors. Teleost fish, including Giant Danio (Devario aequipinnatus), have previously been shown to be glucose intolerant [27]. The experimental studies on hyperglycemia in relation to adrenal cortex are still needed, using fish as experimental model. Therefore, the present study was on the induced hyperglycemic experiment in Giant Danio embryos in relation to effects of hyperglycemia on adrenal cortex function and steroidogenesis. Materials and Methods Collection, domestication and maintenance of fishesThe wild specimens of Devario aequipinnatus were collected the Cauvery river at Stanley Reservoir, (N 11° 54'18.3" E 77° 53' 15.8") Salem District, Tamil Nadu, India, during September to March 2016, using drag net (Figure 1). The live fish samples were transported to the Laboratory in aerated bags and were maintained in a 700 L FRB tank with artificial, continuous aeration at a room temperature of 27-30 °C. The collected fishes, were stocked in rearing tanks of size 1.5 m x 0.5m x 1.5 m and mature males and females were kept separately with the density of 6-7 in each tank. The rearing tanks were provided with dark substrate (gravel and pebbles) and mild circulating water current with the help of electric motors for their adaptation. After proper acclimatization and maintenance, the domesticated brood stock was selected for the breeding experiments. The glass tank (1.5 m x 0.5 m x 1.5 m) with dark substrate and natural day lighting provided with spawning m...

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Trans-species polymorphism of class II Mhc loci in danio fishes

Cited by 26 publications

References 15 publications

Allelic polymorphism, gene duplication and balancing selection of the MHC class II DAB gene of Cynoglossus semilaevis (Cynoglossidae)

Allelic polymorphism, gene duplication and balancing selection of the MHC class II DAB gene of Cynoglossus semilaevis (Cynoglossidae)

Cloning and characterization of major histocompatibility complex class II genes in the stone flounder Kareius bicoloratus (Pleuronectidae)

The Effects Induced Hyperglycemia on Adrenal Cortex Function in the Giant Danio Devario Aequipinnatus Embryos

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