Trans-acting small interfering RNA4: key to nutraceutical synthesis in grape development?

Rock, Christopher D.

doi:10.1016/j.tplants.2013.07.006

Cited by 51 publications

(53 citation statements)

References 155 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When the phased regions were examined using the NCBI BLAST database the majority of these aligned against disease-responsive genes, particularly genes belonging to protein families with nucleotide binding site leucine-rich repeats (NB-LRR) domains. The production of phasiRNAs was demonstrated earlier for NB-LRR protein families [20], [50], [53]–[55], as well as for pentatricopeptide repeat (PPR) [50], [52], [53], [56], MYB [5], [57], [58] and Auxin Signalling F-Box (AFB) protein families [5], [50]. All of these protein families also displayed phasiRNA generation in this study.…”

Section: Resultssupporting

confidence: 67%

Extending the sRNAome of Apple by Next-Generation Sequencing

et al. 2014

View full text Add to dashboard Cite

The global importance of apple as a fruit crop necessitates investigations into molecular aspects of the processes that influence fruit quality and yield, including plant development, fruit ripening and disease resistance. In order to study and understand biological processes it is essential to recognise the range of molecules, which influence these processes. Small non-coding RNAs are regulatory agents involved in diverse plant activities, ranging from development to stress response. The occurrence of these molecules in apple leaves was studied by means of next-generation sequencing. 85 novel microRNA (miRNA) gene loci were predicted and characterized along with known miRNA loci. Both cis- and trans-natural antisense transcript pairs were identified. Although the trans-overlapping regions were enriched in small RNA (sRNA) production, cis-overlaps did not seem to agree. More than 150 phased regions were also identified, and for a small subset of these, potential miRNAs that could initiate phasing, were revealed. Repeat-associated siRNAs, which are generated from repetitive genomic regions such as transposons, were also analysed. For this group almost all available repeat sequences, associated with the apple genome and present in Repbase, were found to produce siRNAs. Results from this study extend our current knowledge on apple sRNAs and their precursors significantly. A rich molecular resource has been created and is available to the research community to serve as a baseline for future studies.

show abstract

Section: Resultssupporting

confidence: 67%

Extending the sRNAome of Apple by Next-Generation Sequencing

et al. 2014

View full text Add to dashboard Cite

show abstract

“…These siRNAs, called trans -acting siRNAs or ta-siRNAs, are produced by DCL4 from dsDNA that is generated by the action of Suppressor of Gene Silencing 3 (SGS3) on the miRNA-cleaved TAS transcript following its transcription by RNA-dependent RNA polymerase 6 (RDR6) (Liu et al 2014; Rock 2013). The miRNA-mediated cleavage of the TAS gene transcript sets the registration point for phasing.…”

Section: Mirna and Stress Regulation Mechanismsmentioning

confidence: 99%

Abiotic stress miRNomes in the Triticeae

Alptekin

Langridge

Budak

2016

Funct Integr Genomics

View full text Add to dashboard Cite

The continued growth in world population necessitates increases in both the quantity and quality of agricultural production. Triticeae members, particularly wheat and barley, make an important contribution to world food reserves by providing rich sources of carbohydrate and protein. These crops are grown over diverse production environments that are characterized by a range of environmental or abiotic stresses. Abiotic stresses such as drought, heat, salinity, or nutrient deficiencies and toxicities cause large yield losses resulting in economic and environmental damage. The negative effects of abiotic stresses have increased at an alarming rate in recent years and are predicted to further deteriorate due to climate change, land degradation, and declining water supply. New technologies have provided an important tool with great potential for improving crop tolerance to the abiotic stresses: microRNAs (miRNAs). miRNAs are small regulators of gene expression that act on many different molecular and biochemical processes such as development, environmental adaptation, and stress tolerance. miRNAs can act at both the transcriptional and post-transcriptional levels, although post-transcriptional regulation is the most common in plants where miRNAs can inhibit the translation of their mRNA targets via complementary binding and cleavage. To date, expression of several miRNA families such as miR156, miR159, and miR398 has been detected as responsive to environmental conditions to regulate stress-associated molecular mechanisms individually and/or together with their various miRNA partners. Manipulation of these miRNAs and their targets may pave the way to improve crop performance under several abiotic stresses. Here, we summarize the current status of our knowledge on abiotic stress-associated miRNAs in members of the Triticeae tribe, specifically in wheat and barley, and the miRNA-based regulatory mechanisms triggered by stress conditions. Exploration of further miRNA families together with their functions under stress will improve our knowledge and provide opportunities to enhance plant performance to help us meet global food demand.

show abstract

“…The fundamental question arises about whether only cis-acting elements present in the promoters of these MYBs are responsible for differential expression or whether any other trans-factors also determine their regulatory mechanisms. Numerous past studies have suggested that miR858 can target R2R3-MYBs in apple, grape, and S. miltiorrhiza (Xia et al, 2012;Rock, 2013;Li et al, 2014). In a quest for such factors, we have shown that miR858a controls the expression of flavonol-specific MYBs, such as MYB11, MYB12, and MYB111 in addition to other members of the MYB family, and studied its detailed role further in Arabidopsis.…”

mentioning

confidence: 91%

“…(Lu et al, 2013;Wang et al, 2014). It has been reported that the miR159, miR319, and miR828 families deregulate MYB transcription factors in apple (Malus domestica), tomato (Solanum lycopersicum), grape (Vitis vinifera), and Salvia miltiorrhiza (Xia et al, 2012;Rock, 2013;Li et al, 2014;Jia et al, 2015). Similarly, a study demonstrated that miR828 regulates MYB2, which is involved in Arabidopsis trichome and cotton (Gossypium hirsutum) fiber development (Guan et al, 2014).…”

mentioning

confidence: 99%

MicroRNA858 Is a Potential Regulator of Phenylpropanoid Pathway and Plant Development

et al. 2016

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are endogenous, noncoding small RNAs that function as critical regulators of gene expression. In plants, miRNAs have shown their potential as regulators of growth, development, signal transduction, and stress tolerance. Although the miRNA-mediated regulation of several processes is known, the involvement of miRNAs in regulating secondary plant product biosynthesis is poorly understood. In this study, we functionally characterized Arabidopsis (Arabidopsis thaliana) miR858a, which putatively targets R2R3-MYB transcription factors involved in flavonoid biosynthesis. Overexpression of miR858a in Arabidopsis led to the down-regulation of several MYB transcription factors regulating flavonoid biosynthesis. In contrast to the robust growth and early flowering of miR858OX plants, reduction of plant growth and delayed flowering were observed in Arabidopsis transgenic lines expressing an artificial miRNA target mimic (MIM858). Genome-wide expression analysis using transgenic lines suggested that miR858a targets a number of regulatory factors that modulate the expression of downstream genes involved in plant development and hormonal and stress responses. Furthermore, higher expression of MYBs in MIM858 lines leads to redirection of the metabolic flux towards the synthesis of flavonoids at the cost of lignin synthesis. Altogether, our study has established the potential role of light-regulated miR858a in flavonoid biosynthesis and plant growth and development.

show abstract

Trans-acting small interfering RNA4: key to nutraceutical synthesis in grape development?

Cited by 51 publications

References 155 publications

Extending the sRNAome of Apple by Next-Generation Sequencing

Extending the sRNAome of Apple by Next-Generation Sequencing

Abiotic stress miRNomes in the Triticeae

MicroRNA858 Is a Potential Regulator of Phenylpropanoid Pathway and Plant Development

Contact Info

Product

Resources

About