‘Traffic light’ immunochromatographic test based on multicolor quantum dots for the simultaneous detection of several antibiotics in milk

Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

“…This effort is testified also by the number of papers reporting new materials to be used as labels [10][11][12][39][40], sometimes in combination with innovative detection technologies [13,41], and of enhancement strategies aimed at increasing the sensitivity of the ICST compared to using traditional gold nanoparticles [42][43], as also summarized in two comprehensive reviews [44][45]. Within the number of novel materials for labeling antibodies in ICTS, the request for analytical devices with multiplexing capability is prompting towards using labels easily distinguishable from each other, such as nanoparticles with different colors [19][20]23]. Gold nanoparticles are widely employed as labels for ICST, thanks to well-recognized advantages, such as easy preparation, stability, easy conjugation to antibodies, brilliant and tunable color [6][7][8]45].…”

“…In this context, assigning different colors to different analytes would make easier and more intuitive the visual interpretation of the results provided by a multiplex dipstick ICST. Color-encoded ICSTs in the multiplex format have been described, which exploit the unique spectral characteristics of quantum dots (QDs) [19][20]. These inorganic nanocrystals are capable of emitting fluorescent light at different wavelengths depending on their size and composition, when excited by the same source.…”

“…These inorganic nanocrystals are capable of emitting fluorescent light at different wavelengths depending on their size and composition, when excited by the same source. Therefore, Taranova et al used QDs emitting at different wavelengths for labeling three antibodies and simultaneously determining three classes of antibiotics by a multiplex ICST [19]. Similarly, Foubert et al used three differently emitting QDs for labeling as many antibodies and to set an ICST for the multiple determinations of four mycotoxins [20].…”

Bioconjugation of quantum dots: Review & impact on future application

“…QDs, in particular, have emerged as one of the most promising labels for immunoassay detection because of their photostability and size tunable photoluminescence. They can be used to develop enzyme linked-immunosorbent assays (ELISAs) [4], lateral flow immunoassays (LFIAs) [99], Western blots [59] or in FRET studies [47,100], to name but a few. For biosensing, the greatest potential of QD-conjugates lies in multiplexing [51,99].…”

“…They can be used to develop enzyme linked-immunosorbent assays (ELISAs) [4], lateral flow immunoassays (LFIAs) [99], Western blots [59] or in FRET studies [47,100], to name but a few. For biosensing, the greatest potential of QD-conjugates lies in multiplexing [51,99]. QDs play also an important role in in vitro and in vivo cell imaging [34], in vivo drug delivery, are often used for cellular labeling (cell surface markers and intracellular markers) and have also made a lot of progress and attracted great interest in this area [48].…”