Traditional herbal medicine: Therapeutic potential in acute gouty arthritis

Wang, Siwei; Liu, Wei; Wei, Bowen; Wang, Aihua; Wang, Yiwen; Wang, Wen; Gao, Jingyue; Jin, Yue; Lu, Hang; Ka, Yuxiu; Yue, Qingyun

doi:10.1016/j.jep.2024.118182

Cited by 2 publications

(1 citation statement)

References 232 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 10 Scrophularia ningpoensis Hemsl and Glycyrrhiza uralensis Fisch also has the effect of heat-clearing and detoxifying, it contains many kinds of flavonoids ingredients can influence the expression of inflammatory cytokines, which regulate the NF-κB signaling pathways, in order to realize the anti-inflammatory analgesic effect. 11 , 12 In addition, many clinical trials have shown that taking Yinhua Gout granules can significantly relieve the symptoms of gouty arthritis. For example, Bai et al treated 112 patients with gouty arthritis using YGG and diclofenac sodium extended-release capsules, and the results showed that Yinhua Gout granules had a significant therapeutic effect on gouty arthritis.…”

Section: Introductionmentioning

confidence: 99%

Study on the Underlying Mechanism of Yinhua Gout Granules in the Treatment of Gouty Arthritis by Integrating Transcriptomics and Network Pharmacology

Fan,

Zhai,

Zhang

et al. 2024

DDDT

View full text Add to dashboard Cite

Purpose Yinhua Gout Granules (YGG) is a traditional Chinese medicine preparation with a variety of pharmacological effects, and its clinical efficacy in the treatment of gouty arthritis (GA) has been fully confirmed. However, the pharmacodynamic basis of YGG and its anti-inflammatory mechanism of action in GA are unknown. The objective of this study was to identify the active components and molecular mechanisms of YGG in the treatment of GA. Methods Ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) and network pharmacology were used to identify and predict the potential active ingredients and related signaling pathways. Then, we revealed the anti-GA effects of YGG based on pharmacodynamic experiments in GA rats. Finally, we integrated transcriptomics and network pharmacology to elucidate the potential mechanism of action and verified the putative mechanism by molecular docking, immunohistochemical (IHC) and Western blot. Results We have identified 10 major active components of YGG that may have anti-GA effects, such as ferulic acid, rutin, luteolin, etc. Using molecular docking, we found that 10 major compounds could bind well to TNF, PTGS2, IL-6, IL1β, NOS2 and PTGS1, and the binding energies were all less than −5 kcal/mol. Animal studies have shown that YGG can improve joint inflammation and inflammatory cell infiltration, reduce serum UA, BUN and Cr levels ( p< 0.01), and decrease IL-1β, IL-6, TNF-α, COX-2 and PGE2 levels in synovial tissue ( p< 0.01), which are associated with the pathogenesis of GA. IHC and Western blot results showed that YGG could regulate TLR4/MYD88/NF-κB pathway to inhibit the inflammatory response induced by GA. Conclusion This study found that YGG could not only improve the disease of GA by inhibiting the production of UA in the body, but also target the regulation of TLR4/MYD88/NF-κB signaling pathway through a variety of active components to achieve effective therapeutic effects on GA.

show abstract

Section: Introductionmentioning

confidence: 99%

Study on the Underlying Mechanism of Yinhua Gout Granules in the Treatment of Gouty Arthritis by Integrating Transcriptomics and Network Pharmacology

Fan,

Zhai,

Zhang

et al. 2024

DDDT

View full text Add to dashboard Cite

show abstract

Syn-COM: A Multi-Level Predictive Synergy Framework for Innovative Drug Combinations

Shi,

Liu,

Guan

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

Drug prediction and treatment using bioinformatics and large-scale modeling have emerged as pivotal research areas. This study proposes a novel multi-level collaboration framework named Syn-COM for feature extraction and data integration of diseases and drugs. The framework aims to explore optimal drug combinations and interactions by integrating molecular virtuality, similarity clustering, overlap area, and network distance. It uniquely combines the characteristics of Chinese herbal medicine with clinical experience and innovatively assesses drug interaction and correlation through a synergy matrix. Gouty arthritis (GA) was used as a case study to validate the framework’s reliability, leading to the identification of an effective drug combination for GA treatment, comprising Tamaricis Cacumen (Si = 0.73), Cuscutae Semen (Si = 0.68), Artemisiae Annuae Herba (Si = 0.62), Schizonepetae Herba (Si = 0.73), Gleditsiae Spina (Si = 0.89), Prunellae Spica (Si = 0.75), and Achyranthis Bidentatae Radix (Si = 0.62). The efficacy of the identified drug combination was confirmed through animal experiments and traditional Chinese medicine (TCM) component analysis. Results demonstrated significant reductions in the blood inflammatory factors IL1A, IL6, and uric acid, as well as downregulation of TGFB1, PTGS2, and MMP3 expression (p < 0.05), along with improvements in ankle joint swelling in GA mice. This drug combination notably enhances therapeutic outcomes in GA by targeting key genes, underscoring the potential of integrating traditional medicine with modern bioinformatics for effective disease treatment.

show abstract

Traditional herbal medicine: Therapeutic potential in acute gouty arthritis

Cited by 2 publications

References 232 publications

Study on the Underlying Mechanism of Yinhua Gout Granules in the Treatment of Gouty Arthritis by Integrating Transcriptomics and Network Pharmacology

Study on the Underlying Mechanism of Yinhua Gout Granules in the Treatment of Gouty Arthritis by Integrating Transcriptomics and Network Pharmacology

Syn-COM: A Multi-Level Predictive Synergy Framework for Innovative Drug Combinations

Contact Info

Product

Resources

About