2009
DOI: 10.1128/aem.02829-08
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Hexachlorobenzene (HCB) has been widely used in chemical manufacturing processes and as a pesticide. Due to its resistance to biological degradation, HCB has mainly accumulated in freshwater bodies and agricultural soils. "Dehalococcoides" spp., anaerobic dechlorinating bacteria that are capable of degrading HCB, were previously isolated from river sediments. Yet there is limited knowledge about the abundance, diversity, and activity of this genus in the environment. This study focused on the molecular analysi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

2
34
0

Year Published

2009
2009
2019
2019

Publication Types

Select...
7
2
1

Relationship

2
8

Authors

Journals

citations
Cited by 42 publications
(36 citation statements)
references
References 58 publications
2
34
0
Order By: Relevance
“…According to SiREM, the primers used for DNA replication during PCR were 5 0 -ATTACCGCGGCTGCTGG-3 0 and 5 0 -CGCCCGC CGCGCGCGGCGGGCGGGGCGGGGGCACGGGG GGCCTACGGGAGGCAGCAG-3 0 , following Muyzer et al (1993). It should be noted that different strains of Dehalococcoides with the same 16S rRNA gene sequence can have different dehalogenating abilities, and therefore targeting strain-specific reductive dehalogenase functional genes (e.g., vcrA and/or bvcA) is necessary to provide information about actual dechlorination activity (Ritalahti et al 2006;Lookman et al 2007;Behrens et al 2008;Cupples 2008;Tas et al 2009). However, use of 16S rRNA for quantifying Dehalococcoides is an established technique (e.g., Cupples et al 2003;Lendvay et al 2003;Smits et al 2004;Buergmann et al 2008;Cupples 2008) and was deemed appropriate for the analysis presented here.…”
Section: Microbiological Analysismentioning
confidence: 99%
“…According to SiREM, the primers used for DNA replication during PCR were 5 0 -ATTACCGCGGCTGCTGG-3 0 and 5 0 -CGCCCGC CGCGCGCGGCGGGCGGGGCGGGGGCACGGGG GGCCTACGGGAGGCAGCAG-3 0 , following Muyzer et al (1993). It should be noted that different strains of Dehalococcoides with the same 16S rRNA gene sequence can have different dehalogenating abilities, and therefore targeting strain-specific reductive dehalogenase functional genes (e.g., vcrA and/or bvcA) is necessary to provide information about actual dechlorination activity (Ritalahti et al 2006;Lookman et al 2007;Behrens et al 2008;Cupples 2008;Tas et al 2009). However, use of 16S rRNA for quantifying Dehalococcoides is an established technique (e.g., Cupples et al 2003;Lendvay et al 2003;Smits et al 2004;Buergmann et al 2008;Cupples 2008) and was deemed appropriate for the analysis presented here.…”
Section: Microbiological Analysismentioning
confidence: 99%
“…For example, in a study of sediments contaminated with 1,2-dichloroethane (1,2-DCA), the abundance of Dehalococcoides did not correlate with the presence or absence of 1,2-DCA dechlorination (45). In another study of the halorespiration of chlorinated benzenes, the abundance of Dehalococcoides in contaminated river sediment did not correlate significantly with the amount of hexachlorobenzene in situ (42). Furthermore, in follow-on research by the same investigators, Dehalococcoides-like organisms from fresh sediment grew 2 orders of magnitude in batch cultures before dechlorination of amended chlorobenzenes was detected (43).…”
mentioning
confidence: 99%
“…Currently, this technology has become a powerful and high-throughput tool for analyzing microbial communities and monitoring environmental processes and ecosystem functions (c.f., Wu et al, 2001Wu et al, , 2006Wu et al, , 2008Loy et al, 2002;TaroncherOldenburg et al, 2003;Bodrossy and Sessitsch, 2004;Rhee et al, 2004;Steward et al, 2004;Tiquia et al, 2004;Dix et al, 2006;Rodriguez-Martinez et al, 2006;He et al, 2007;Leigh et al, 2007;Yergeau et al, 2007;Zhou et al, 2008;Liang et al, 2009;Mason et al, 2009;Tas et al, 2009;Van Nostrand et al, 2009;Waldron et al, 2009;Wang et al, 2009). Especially, one of our previous functional gene arrays (FGAs), GeoChip 2.0, containing more than 24 000 probes and covering more than 10 000 gene sequences from B150 gene categories involved in key microbially mediated biogeochemical processes has been widely used to analyze microbial communities from different resources, such as soils (Yergeau et al, 2007;Zhou et al, 2008), waters Leigh et al, 2007;Tas et al, 2009;Van Nostrand et al, 2009;Waldron et al, 2009), oil fields (Liang et al, 2009), marine sediments (Wu et al, 2008), extreme environments (Mason et al, 2009;Wang et al, 2009), bioreactor systems (Rodriguez-Martinez et al, 2006) and other habitats (Kimes et al, 2010), to address a variety of questions related to biogeochemical cycles, bioremediati...…”
Section: Introductionmentioning
confidence: 99%